Development of microfluidic droplet shooter and its application to interface for mass spectrometry

Accelerated protein digestion and separation with picoliter volume utilizing nanofluidics

“…The analysis including digestion and separation was completed within tens of minutes, which is much shorter than the time required for conventional bulk methods. In the future, this integrated device will be able to achieve single-cell proteomics by integrating with a MS source for ionization, 49 cell chambers and nanopillars for cell debris removal. 50…”

Microfluidic nanodevices for drug sensing and screening applications

“…Therefore, mass spectrometry can be used for the identification or confirmation of molecules ( Domon and Aebersold, 2006 ; Sansa et al, 2020 ). The integration of microfluidics with mass spectrometry provides a simple and sensitive analytical workflow process ( Kazoe et al, 2021 ; Liu and Lin, 2016 ; D. Zhang et al, 2021 ) with automation capability, reduced sample preparation ( Liu and Lin, 2016 ), shorter analysis time ( Korzhenko et al, 2021 ), good reusability, high throughput ( Looby et al, 2019 ), high characterization capability ( Shen et al, 2021 ), and easy integration with various isolated instruments ( Spencer et al, 2022 ), thus greatly enhancing the analytical capability. Herein, the integration of mass spectrometry and microfluidics is ideally suited for highly sensitive drug screening.…”

“…Herein, the integration of mass spectrometry and microfluidics is ideally suited for highly sensitive drug screening. Drug screening covers a wide range of topics, including the retention of antibiotics in food ( Wu et al, 2016 ; Zhao et al, 2019 ), monitoring of blood levels of therapeutic drugs for clinical diagnosis ( Kazoe et al, 2021b ; Korzhenko et al, 2021b ; Spencer et al, 2022b ; Tascon et al, 2018 ), and screening of anticancer drugs ( Lin et al, 2016b ; Sun et al, 2020 ; Wu et al, 2016b ).…”

Advances in Single-Cell Printing

“…As mentioned previously, single-cell analysis has become a powerful and indispensable tool in modern biological and medical research [2,3]. With the development of single-cell printing in the past decade, various single-cell printing-based single-cell analyses and applications have been performed, ranging from single-cell array-based screening [89,90,93,[107][108][109] and single-cell based mass spectroscopy [110][111][112][113][114][115] to live three-dimensional tissue formation [47,48,116].…”

“…Mass spectrometry (MS) is a powerful tool to qualitatively and quantitatively detect molecules at the femtomolar sensitivity without a labeling requirement [117]. Furthermore, the multiplex detection with high throughput and low sample consumption makes MS the ideal tool for single-cell analysis [110][111][112][113][114][115], especially for single-cell proteomics [8,118,119].…”