Development of microsatellite markers in Robinsonia (Asteraceae) an endemic genus of the Juan Fernández Archipelago, Chile

Takayama, Kōji; Sepúlveda, Patricio López; Köhl, Gudrun; Novak, Johannes; Stuessy, Tod F.

doi:10.1007/s12686-012-9734-2

Cited by 9 publications

(12 citation statements)

References 16 publications

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“…Previous studies have used NGS technology to identify SSRs from oceanic island endemic plants. Takayama et al (2013Takayama et al ( , 2015 have developed and used 10 SSR markers for Robinsonia DC. (Asteraceae), endemic to the Juan Fernández Archipelago, to the small sample sizes of all the taxa it is hard to explain this; however, this could indicate these species are more closely related than previously discussed, or that some hybridization between these taxa has been uncovered.…”

Section: Methods and Resultsmentioning

confidence: 99%

Transcriptome sequencing and simple sequence repeat marker development for three Macaronesian endemic plant species

et al. 2016

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Premise of the study:Oceanic islands offer unparalleled opportunities to investigate evolutionary processes such as adaptation and speciation. However, few genomic resources are available for oceanic island endemics. In this study, we publish transcriptome sequences from three Macaronesian endemic plant species (Argyranthemum broussonetii [Asteraceae], Descurainia bourgaeana [Brassicaceae], and Echium wildpretii [Boraginaceae]) that are representative of lineages that have radiated in the region. In addition, the utility of transcriptome data for marker development is demonstrated.Methods and Results:Transcriptomes from the three plant species were sequenced, assembled, and annotated. Between 1972 and 2282 simple sequence repeats (SSRs) were identified for each taxon. Primers were designed and tested for 30 of the candidate SSRs identified in Argyranthemum, of which 12 amplified well across three species and eight were polymorphic.Conclusions:We demonstrate here that a single transcriptome sequence is sufficient to identify hundreds of polymorphic SSR markers. The SSRs are applicable to a wide range of questions relating to the evolution of island lineages.

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Section: Methods and Resultsmentioning

confidence: 99%

Transcriptome sequencing and simple sequence repeat marker development for three Macaronesian endemic plant species

et al. 2016

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“…) and a slightly modified protocol of the Qiagen Multiplex PCR Kit (Qiagen) following Takayama et al . (). A total of 280 individuals, mostly the same individuals used in the AFLP analyses, was analyzed from the two species (Table ).…”

Section: Methodsmentioning

confidence: 97%

“…Nine microsatellite markers isolated from R. venustus were selected based on their repeatability and scoring convenience (Takayama et al 2011). Three combinations of multiplex PCR amplification using different dyes (range of sizes given in parentheses), RV-BA8LD (112-126 bp), RV-ANODC (174-204 bp), RV-A5063 (145-239 bp) with 6-FAM, RV-AON43 (90-104 bp), RV-183A0 (201-211 bp), RV-BFTAL (271-276 bp) with NED, RV-ANEM7 (105-127 bp), RV-BDN33 (190-212 bp), RV-A63BG (268-298 bp), were performed using the 5 0 -tailed primer method (Boutin-Ganache et al 2001) and a slightly modified protocol of the Qiagen Multiplex PCR Kit (Qiagen) following Takayama et al (2013). A total of 280 individuals, mostly the same individuals used in the AFLP analyses, was analyzed from the two species (Table 2).…”

Section: Microsatellites (Ssrs)mentioning

confidence: 99%

Biogeography and genetic consequences of anagenetic speciation ofRhaphithamnus venustus(Verbenaceae) in theJuanFernández archipelago,Chile: insights fromAFLPandSSRmarkers

López-Sepúlveda

Takayama

Crawford

et al. 2016

Plant Species Biology

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The genus R haphithamnus (Verbenaceae) consists of two species, one in South America and another endemic to the Juan Fernández archipelago, Chile. The genus represents an example of anagenetic speciation in which the island populations have diverged from their colonizing ancestors to the point where they are recognized as a distinct species. The island species R haphithamnus venustus differs from the continental R . spinosus primarily by floral traits associated with adaptation to hummingbird pollination. Two molecular markers, amplified fragment length polymorphisms (AFLPs) and microsatellites, were used to estimate divergence between the continental and insular species, and to compare diversity in the two species. The comparable or greater diversity in the insular species observed in some diversity indices of AFLPs would support the hypothesis that during the course of anagenetic speciation it has recovered from any reduction of genetic diversity associated with colonization of the archipelago. This pattern of comparable or higher diversity in insular species is seen with other instances of anagenetic speciation in the Juan Fernández archipelago. By contrast, the lower genetic diversity in the insular R . venustus found in microsatellites is likely to be the result of a founder effect from the original colonization of the archipelago; prior molecular studies suggest recent colonization of the Juan Fernández archipelago by R haphithamnus . The seeming non‐concordance between the present results and the widely accepted biogeography of R haphithamnus inferred from other data is discussed and an explanation is presented.

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“…Además, los autores ponen en duda la monofilia del grupo a partir de análisis de secuencias de ITS-ETS que con muy baja resolución incluyen a R. berteroi en un clado de Senecio continental, separado de las otras Robinsonia de las islas (Pelser et al 2010). Por otro lado, Takayama et al (2013Takayama et al ( , 2014 aún reconocen la validez del género argumentando que morfológica y reproductivamente difiere notablemente de Senecio. Sin embargo, sus estudios no tienen relación con la sistemática filogenética del grupo.…”

Section: Géneros Endémicos Del Archipiélago De Juan Fernández E Isunclassified

Consideraciones sobre la sistemática de las familias y los géneros de plantas vasculares endémicos de Chile

Urbina-Casanova

Saldivia²,

Scherson

2015

Gayana Bot.

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RESUMENEl endemismo es uno de los principales aspectos que trata la biogeografía histórica y es uno de los criterios más importantes para establecer las prioridades de conservación de las especies. En el mundo, más del 90% de las plantas que se encuentra en alguna categoría de amenaza son endémicas de un sólo país. En Chile, un 45% de las especies de plantas vasculares son endémicas. Actualmente este número incluye 83 géneros y 4 familias endémicas del país; éstos son valores elevados en comparación con el resto de Latinoamérica. Sin embargo, la alta tasa de cambios producidos por los estudios de sistemática molecular en la taxonomía ha generado modificaciones en estos números. Este trabajo pretende discutir dichas modificaciones y así contribuir a la correcta delimitación de estos géneros endémicos. Utilizando bases de datos y bibliografía actualizadas, se llevó a cabo una revisión exhaustiva sobre estos géneros. Se sustrajeron de la lista aquellos géneros con registros fuera del país y aquellos que cuentan con evidencia suficiente para cambiar su estatus taxonómico. Se entrega una revisión crítica de la bibliografía sistemática respecto de los géneros en cuestión, discutiendo principalmente aquellos que a la luz de la literatura presentaron ambigüedades en su clasificación. PALABRAS CLAVE:Endemismo, Sistemática molecular, Filogenia molecular, Conservación biológica. ABSTRACTEndemism is one of the main considerations in historical biogeography and one of the most important criteria when establishing conservation priorities. Worldwide, more than 90% of the plants listed in conservation categories are endemic to one country. In Chile, 45% of the flora is endemic, currently considering 83 endemic genera and 4 endemic families, elevated numbers compared to the rest of Latinamerica. However, the high rate of changes in taxonomy, based on studies of molecular systematics has produced modifications in this list. This study aims at discussing such modifications and in this way, contribute to the correct delimitation of our endemic genera. Using updated databases and bibliographic sources, an exhaustive revision of these genera was carried out. Genera with registries outside of the country, as well as those that had enough evidence to change their taxonomic status were subtracted from the list. A critical revision of the systematic bibliography available is provided, emphasizing the discussion on those genera with ambiguous classification status.

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Development of microsatellite markers in Robinsonia (Asteraceae) an endemic genus of the Juan Fernández Archipelago, Chile

Cited by 9 publications

References 16 publications

Transcriptome sequencing and simple sequence repeat marker development for three Macaronesian endemic plant species

Transcriptome sequencing and simple sequence repeat marker development for three Macaronesian endemic plant species

Biogeography and genetic consequences of anagenetic speciation ofRhaphithamnus venustus(Verbenaceae) in theJuanFernández archipelago,Chile: insights fromAFLPandSSRmarkers

Consideraciones sobre la sistemática de las familias y los géneros de plantas vasculares endémicos de Chile

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