Development of microstructured fish scale collagen scaffolds to manufacture a tissue-engineered oral mucosa equivalent

Suzuki, Ayako; Kato, Hiroko; Kawakami, Takahiro; Kodama, Yoshihiro; Shiozawa, Mayuko; Kuwae, Hiroyuki; Miwa, Keito; Hoshikawa, Emi; Haga, Ken; Shiomi, Aki; Uenoyama, Atsushi; Saitoh, Issei; Hayasaki, Haruaki; Mizuno, Jun; Izumi, Kenji

doi:10.1080/09205063.2019.1706147

Cited by 28 publications

(27 citation statements)

References 41 publications

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“…Similar to the previous report, the microstructure configuration of the grid micropattern in this study appeared to be well-preserved, however, the pillar micropattern appeared to be collapsed and poorly preserved, ( Fig. 3B; 1-4) 15 . In contrast, the four types of micropatterns that were fabricated with 100 μm in height, thickness and channel width respectively, were all well-transferred onto the collagen scaffold ( Fig.…”

Section: Fabrication Of Negative Molds For Micropatterns With Varioussupporting

confidence: 90%

“…The diameters of fibrils of the 1% tilapia scale type I collagen matrices with EDC cross-linking ranged from 40 to 120 nm, which were approximately 1.2 times thicker than those without EDC crosslinking shown in the Fig. 3a in our previous report 15 . SEM showed that micropatterns were formed on the collagen scaffolds crosslinked with 1% EDC, with several dimensions and aspect ratio.…”

Section: Fabrication Of Negative Molds For Micropatterns With Variousmentioning

confidence: 47%

“…SEM showed that micropatterns were formed on the collagen scaffolds crosslinked with 1% EDC, with several dimensions and aspect ratio. The surface textures of collagen scaffolds with or without 1% EDC crosslinking showed little differences between all the micropatterns 15 . Similar to the previous report, the microstructure configuration of the grid micropattern in this study appeared to be well-preserved, however, the pillar micropattern appeared to be collapsed and poorly preserved, ( Fig.…”

Section: Fabrication Of Negative Molds For Micropatterns With Variousmentioning

confidence: 94%

“…6A,B). The dimension and aspect ratio of micropatterning were identical to the previous study design 15 . The original microstructures fabricated on the scaffold surface crosslinked with 1% EDC were relatively well-preserved, in spite of a slight decrease in the vertical dimension .…”

Section: Macroscopic Findings Of Evpomes During Manufacturing Contramentioning

confidence: 99%

“…the connective tissue papilla of oral mucosa, we previously designed two grid-and pillar-micropattern prototypes with rectangular or truncated configurations, respectively, resulting in four negative molds as micropatterns, and their dimensions on the scaffold designed were approximately 200 μm in height, 200 μm in thickness, and 100 μm in channel width 15 . To test the conformability of our manufacturing system, 15 different dimensions and aspect ratios smaller than those of previous study, including the same four micropatterns (i.e., ratio of topography height to topography thickness), were manufactured.…”

Section: Design Of Micropatterns and Fabrication Of Microstructured Nmentioning

confidence: 99%

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Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

Suzuki

Kodama²,

Miwa

et al. 2020

Sci Rep

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The junction between the epithelium and the underlying connective tissue undulates, constituting of rete ridges, which lack currently available soft tissue constructs. In this study, using a micro electro mechanical systems process and soft lithography, fifteen negative molds, with different dimensions and aspect ratios in grid- and pillar-type configurations, were designed and fabricated to create three-dimensional micropatterns and replicated onto fish-scale type I collagen scaffolds treated with chemical crosslinking. Image analyses showed the micropatterns were well-transferred onto the scaffold surfaces, showing the versatility of our manufacturing system. With the help of rheological test, the collagen scaffold manufactured in this study was confirmed to be an ideal gel and have visco-elastic features. As compared with our previous study, its mechanical and handling properties were improved by chemical cross-linking, which is beneficial for grafting and suturing into the complex structures of oral cavity. Histologic evaluation of a tissue-engineered oral mucosa showed the topographical microstructures of grid-type were well-preserved, rather than pillar-type, a well-stratified epithelial layer was regenerated on all scaffolds and the epithelial rete ridge-like structure was developed. As this three-dimensional microstructure is valuable for maintaining epithelial integrity, our micropatterned collagen scaffolds can be used not only intraorally but extraorally as a graft material for human use.

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Section: Fabrication Of Negative Molds For Micropatterns With Varioussupporting

confidence: 90%

Section: Fabrication Of Negative Molds For Micropatterns With Variousmentioning

confidence: 47%

Section: Fabrication Of Negative Molds For Micropatterns With Variousmentioning

confidence: 94%

Section: Macroscopic Findings Of Evpomes During Manufacturing Contramentioning

confidence: 99%

Section: Design Of Micropatterns and Fabrication Of Microstructured Nmentioning

confidence: 99%

See 3 more Smart Citations

Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

Suzuki

Kodama²,

Miwa

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

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Vascularization strategies in tissue engineering approaches for soft tissue repair

Masson-Meyers

Tayebi

2021

J Tissue Eng Regen Med

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Insufficient vascularization during tissue repair is often associated with poor clinical outcomes. This is a concern especially when patients have critical-sized injuries, where the size of the defect restricts vascularity, or even in small defects that have to be treated under special conditions, such as after radiation therapy (relevant to tumor resection) that hinders vascularity. In fact, poor vascularization is one of the major obstacles for clinical application of tissue engineering methods in soft tissue repair. As a key issue, lack of graft integration, caused by inadequate vascularization after implantation, can lead to graft failure. Moreover, poor vascularization compromises the viability of cells seeded in deep portions of scaffolds/graft materials, due to hypoxia and insufficient nutrient supply. In this article we aim to review vascularization strategies employed in tissue engineering techniques to repair soft tissues. For this purpose, we start by providing a brief overview of the main events during the physiological wound healing process in soft tissues. Then, we discuss how tissue repair can be achieved through tissue engineering, and considerations with regards to the choice of scaffold materials, culture conditions, and vascularization techniques. Next, we highlight the importance of vascularization, along with strategies and methods of prevascularization of soft tissue equivalents, particularly cellbased prevascularization. Lastly, we present a summary of commonly used in vitro methods during the vascularization of tissue-engineered soft tissue constructs.

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Sustainable Materials for Biomedical Engineering Application in Dentistry

Law

Yazid

2023

Sustainable Material for Biomedical Engineering Application

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Development of microstructured fish scale collagen scaffolds to manufacture a tissue-engineered oral mucosa equivalent

Cited by 28 publications

References 41 publications

Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

Vascularization strategies in tissue engineering approaches for soft tissue repair

Sustainable Materials for Biomedical Engineering Application in Dentistry

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