Development of MoAb HMSA-3 and HMSA-4 Against Human Melanoma Melanosomes and Their Reactivities on Formalin-Fixed Melanoma Tissues

Maeda, Kazuo; Yamana, K; Jimbow, Kowichi

doi:10.1111/1523-1747.ep12461297

Cited by 8 publications

(2 citation statements)

References 16 publications

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“…Therefore, a detailed comparison of four MoAbs was made in four major subtypes of malignant melanoma; acral lentiginous melanoma, superficial spreading melanoma, lentigo maligna melanoma, and nodular melanoma. In all cases of primary melanoma studied, one of the four MoAbs always showed a positive reactivity, even though the other three were negative or weakly positive [41]. Our immunohistopathologic study indicated that HMSA provides a unique marker to identify malignant melanoma cells and may be even useful to delineate the subtypes of malignant melanoma with the HMSA expression [40].…”

Section: Unique Expression Of Melanosomal Proteins In Normal Melanocymentioning

confidence: 63%

Melanin Pigments and Melanosomal Proteins as Differentiation Markers Unique to Normal and Neoplastic Melanocytes.

Jimbow¹,

Lee²,

King³

et al. 1993

J Invest Dermatol

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This report introduces some aspects of our current basic research focus on the unique metabolic pathways within the melanocyte. Using this approach, we hope to gain a better understanding of the pathophysiology of malignant melanoma and develop early laboratory diagnostic tests for this disease. Specifically, we will discuss that: 1) the synthesis of pheomelanin is markedly increased in malignant melanoma and dysplastic melanocytic nevi; 2) high levels of metabolites of pheomelanin and eumelanin can be detected in the urine and blood of patients with metastatic melanoma; 3) this release of melanin metabolites appears to correlate with tumor thickness and tumor load, including the extent of metastasis; 4) the synthesis of melanosomal proteins also becomes aberrant in malignant melanoma; and 5) this abnormal melanosome synthesis can be utilized in the identification of antigenic epitopes that are uniquely expressed in malignant melanoma. We believe that this synthesis and secretion of abnormal melanin pigment and melanosomal proteins (human melanosome-specific antigen) would be useful for the development of early laboratory diagnostic and monitoring tools for malignant melanoma. In addition, we also report the detection of pheomelanin component in "normal" unexposed skin; however, the relative amount of pheomelanin in the skin does not reflect hair color (e.g., red hair). The nature of this pheomelanin component in the skin needs to be further clarified.

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Section: Unique Expression Of Melanosomal Proteins In Normal Melanocymentioning

confidence: 63%

Melanin Pigments and Melanosomal Proteins as Differentiation Markers Unique to Normal and Neoplastic Melanocytes.

Jimbow¹,

Lee²,

King³

et al. 1993

J Invest Dermatol

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“…A series of monoclonal antibodies has been raised against human melanosomal proteins and tested for spec ificity of reaction [76][77][78][79], It has been known for some time that meianosomes in malignant melanocytes are aberrant in appearance, and that specific immune re sponses have been generated against them (see above). In general, those antibodies react positively with cytoplas mic antigens in malignant melanoma (and some with dysplastic nevus) cells, but do not react with other tissue, including normal melanocyte populations.…”

Section: Hmsa Antigensmentioning

confidence: 99%

Antigens of Murine Melanoma and Their Cross-Species Reactivity

Gersten

Hearing

1992

Pathobiology

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Many investigators have taken a two-stage approach to the study of tumor antigens. The first is the evaluation of antigens produced by animal tumors and the second is the determination of the extent to which animal antigens have relevant human homologs. Accordingly, we discuss the known protein antigens of murine melanoma with emphasis on those expressed by more than one species. These include six mouse-specific and five cross-species antigens, and 1 human antigen transfected into mouse cells. Of the five cross-species antigens, B700 and HMW have been demonstrated in four different species, making them candidate ‘pan-melanoma’ antigens.

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Complementary expression of melanosomal antigens and constant expression of pigment-independent antigen during the evolution of melanocytic tumours

Takahashi

Parsons

Favier

et al. 1990

Vichows Archiv A Pathol Anat

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We have generated monoclonal antibodies (MoAbs) against melanosomal proteins (MoAb 1C11 and MoAb HMSA-1) and a cytoplasmic protein strongly synthesized in neoplastic melanocytes but not associated with melanogenesis (MoAb 7H11). An immunohistochemical study of paraffin sections showed that nearly 90% of epidermal neoplastic melanocytes, including melanomas, expressed 1C11 antigen, whereas this antigen was poorly preserved in dermal melanocytic cells except melanomas. HMSA-1 antigen was expressed in a complementary manner to 1C11 antigen, being found in dermal naevus cells but not generally in the epidermal regions, except for dysplastic naevi and melanomas. In contrast, 7H11 antigen was distributed in nearly 90% of melanocytic tumours except solar lentigo and lentigo maligna lesions. The failure of MoAb 1C11 to react with dermal melanocytes may reflect a subtle alteration in melanogenesis during tumour evolution. Overall, the combined use of MoAbs serves as an accurate diagnosis of melanocytic tumours, the pigment-independent MoAb 7H11 being particularly useful for amelanotic and metastatic lesions.

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Development of MoAb HMSA-3 and HMSA-4 Against Human Melanoma Melanosomes and Their Reactivities on Formalin-Fixed Melanoma Tissues

Cited by 8 publications

References 16 publications

Melanin Pigments and Melanosomal Proteins as Differentiation Markers Unique to Normal and Neoplastic Melanocytes.

Melanin Pigments and Melanosomal Proteins as Differentiation Markers Unique to Normal and Neoplastic Melanocytes.

Antigens of Murine Melanoma and Their Cross-Species Reactivity

Complementary expression of melanosomal antigens and constant expression of pigment-independent antigen during the evolution of melanocytic tumours

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