Development of Molecular Imprinting Polymer Nanofiber for Aflatoxin B1 Detection Based on Quartz Crystal Microbalance

Abstract

Aflatoxin B1 (AFB1) is one of the mycotoxins with the most dangerous poisons and poses a threat to living things. Several detection methods for Aflatoxin B1 (AFB1) with high sensitivity (LC-MS technique, HPLC, ELISA, etc.) still require lengthy preparation time and are not real-time and portable. Aflatoxin B1 (AFB1) detection is one of the major challenges in the field of food safety because Aflatoxin B1 (AFB1) attacks the food and agricultural products sector. One of the potential sensors that can be used as a base for Aflatoxin B1 (AFB1) detection is the Quartz Crystal Microbalance (QCM) sensor. This study examines the performance of the Quartz Crystal Microbalance (QCM) sensor as one of the Aflatoxin B1 detection techniques through the physical deposition method. The Quartz Crystal Microbalance (QCM) sensor modified uses polyvinyl acetate (PVAc) material as a container to embed a molecular model that will be detected through a molecular imprinting polymer (MIP) process coated on QCM using the electrospinning method. The response results show that the value of the sensor response using the MIP process is more significant than without the MIP process. The sensor characteristics demonstrated by the PVAc/AFB 50 sample have a limit of detection (LOD) value is 0.63 ppb, and a limit of quantitation (LOQ) is 1.91 ppb with a coefficient correlation is 0.97 for testing with a concentration range of 5.0 – 40.0 ppb. Therefore, the MIP process in QCM provides a favorable response for the detection of AFB1 in the future.

Ensuring food safety with molecularly imprinted polymers: innovative methods for the detection of aflatoxins in food and feed samples

“…A mass-sensitive transducer such as a quartz crystal microbalance (QCM) has been combined with MIPs to monitor aflatoxins (Susilo et al, 2021). The gadget measures surface-analyte interactions by monitoring the changes in resonance frequency (Mujahid et al, 2019), and has several advantages including high sensitivity and real-time output.…”

Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review

“…The number of commercial biosensors that can detect the interaction of receptors with their targets in a preferably label-free manner and on time is steadily growing (Table 7). Important detection techniques are surface plasmon resonance (SPR) [403,404], quartz crystal microbalance (QCM) [405,406], microcantilever arrays [407], biolayer interferometry (BLI) [408], and electroswitchable biosurfaces (ESB) [409,410].…”