Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress

Gupta, Debjyoti Sen; McPhee, Kevin; Kumar, Shiv

doi:10.3389/fpls.2017.00579

Cited by 15 publications

(7 citation statements)

References 28 publications

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“…In wild and cultivated wheat, variability in grain for Fe and Zn contents have been found related to allelic variation at a chromosomal locus involved in remobilization of protein, Fe, Zn and Mn from senescing leaves to seeds [ 21 ]. Recently, Gupta et al [ 22 ] developed markers for some iron metabolism related genes ( Ferritin-1 , BHLH-1 , IRT-1 ) in lentil. Thus, the intra- as well as inter-specific variability observed for seed elemental composition in the present study also highlights the hidden role of tight genetic control besides influence of plant morphology.…”

Section: Discussionmentioning

confidence: 99%

Bio-fortification potential of global wild annual lentil core collection

et al. 2018

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Lentil, generally known as poor man’s’ meat due to its high protein value is also a good source of dietary fiber, antioxidants and vitamins along with fast cooking characteristics. It could be used globally as a staple food crop to eradicate hidden hunger, if this nutritionally rich crop is further enriched with essential minerals. This requires identification of essential mineral rich germplasm. So, in the present study, a core set of 96 wild accessions extracted from 405 global wild annual collections comprising different species was analyzed to determine its bio-fortification potential. Impressive variation (mg/100 g) was observed for different minerals including Na (30–318), K (138.29–1578), P (37.50–593.75), Ca (4.74–188.75), Mg (15–159), Fe (2.82–14.12), Zn (1.29–12.62), Cu (0.5–7.12), Mn (1.22–9.99), Mo (1.02–11.89), Ni (0.16–3.49), Pb (0.01–0.58), Cd (0–0.03), Co (0–0.63) and As (0–0.02). Hierarchical clustering revealed high intra- and inter-specific variability. Further, correlation study showed positive significant association among minerals and between minerals including agro-morphological traits. Accessions representation from Turkey and Syria had maximum variability for different minerals. Diversity analysis exhibited wide geographical variations across gene-pool in core set. Potential use of the identified trait-specific genetic resources could be initial genetic material, for genetic base broadening and biofortification of cultivated lentil.

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Section: Discussionmentioning

confidence: 99%

Bio-fortification potential of global wild annual lentil core collection

et al. 2018

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“…As a legume species, the roots of lentils can induce the reduction of Fe and Mn by the ferric reductase enzyme expressed on the epidermal cells of roots, which can greatly help the root system to absorb cation micronutrients [23][24][25]. Furthermore, because lentils can fix N 2 in symbiosis with rhizobia, there is an unbalanced uptake of cations that results in pumping H + to the rhizosphere to maintain the membrane potential in normal metabolic ranges, triggering the acidification of the rhizosphere [26,27].…”

Section: Introductionmentioning

confidence: 99%

Management of Iron and Manganese Toxicities of Lentil Crops Grown in Central Chile

et al. 2021

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Iron (Fe) and manganese (Mn) toxicity is a widespread problem in lentil production in the coastal dryland of Chile. Increasing the soil pH by liming with CaCO3 or incrementing grain yields through nitrogen fertilization can help the plants to reduce metal concentration. Thus, the main objective of this work was to evaluate two different fertilization strategies (lime (CaCO3) and nitrogen (N) additions) to reduce Fe and Mn toxicities in lentils. Lentils grown under field conditions with the highest Fe and Mn concentrations showed toxicity symptoms, but without grain yield reductions. In a pot experiment using the same soil as in the field with toxicity symptoms, the dry matter (DM) produced at the end of the trial was higher in the plants that received N while the lowest DM production was recorded in those plants treated with lime. In particular, higher root DM sustained the growth of the N-fertilized shoots, which also positively affected the grain yields being 33% higher than the control treatment (no fertilization addition). In the plants fertilized with N, the Fe and Mn levels in the shoots were lower than the control plants and those grown in soils treated with lime, but showed higher concentrations of Fe and Mn in roots. In parallel, roots exhibited high concentrations of Fe and Mn that were 13- and 9-fold higher than in the shoots. Additionally, a significant decrease of 29% in Mn concentration in the grains of plants treated with N was reported. Overall, our results suggest that an increase in DM of lentils by the addition of N can reduce the Mn concentration on leaves to a level that is likely under the threshold that causes toxicity in plant tissues. Finally, we conclude that the increase of Fe and Mn in the roots may be connected to the reduction of these metals on leaves.

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Section: Resultsmentioning

confidence: 99%

“…Ferritins are carriers that regulate iron homeostasis where bound iron is readily available (Gupta et al, 2017). Ferritin‐1 increased in lentil roots ( Lens culinaris ) under excess iron, but iron treatments had no impact on shoot ferritins (Gupta et al, 2017). GRXs are thiol‐based oxidoreductases that reduce protein disulfide bridges using GSH and may partake in direct ROS scavenging (Wu et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Differential protein abundance of vetiver grass in response to acid mine drainage

Kiiskila

Sarkar

Datta

2021

Physiologia Plantarum

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Acid mine drainage (AMD) is an acidic and metalliferous discharge that imposes oxidative stress on living things through bioaccumulation and physical exposure. The abandoned Tab-Simco mining site of Southern Illinois generates highly acidic AMD with elevated sulfate (SO 4 2À ) and various metals. Vetiver grass (Chrysopogon zizanioides) is effective for the remediation of Tab-Simco AMD at both mesocosm and microcosm levels over extended periods. In this study, we conducted a proteomic investigation of vetiver shoots under short and long-term exposure to AMD. Our objective was to decipher the physiological responses of vetiver to the combined abiotic stresses of AMD (metal and low pH). Differential regulation was observed for longer-term (56 days) exposure to AMD, which resulted in 17 upregulated and nine downregulated proteins, whereas shorter-term (7 days) exposure led to 14 upregulated and 14 downregulated proteins. There were significant changes to

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Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress

Cited by 15 publications

References 28 publications

Bio-fortification potential of global wild annual lentil core collection

Bio-fortification potential of global wild annual lentil core collection

Management of Iron and Manganese Toxicities of Lentil Crops Grown in Central Chile

Differential protein abundance of vetiver grass in response to acid mine drainage

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