Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset

Kametani, Yoshie; Suzuki, Daisuke; Kohu, Kazuyoshi; Satake, Masanobu; Suemizu, Hiroshi; Sasaki, Erika; Ito, Toshio; Tamaoki, Norikazu; Mizushima, Tomoko; Ozawa, Manabu; Tani, Kenzaburo; Kito, Mitsuaki; Arai, Hideo; Koyanagi, Akemi; Yagita, Hideo; Habu, Sonoko

doi:10.1016/j.exphem.2009.08.003

Cited by 19 publications

(21 citation statements)

References 42 publications

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“…On the other hand, CM PBMCs expressed high levels of IFN-γ mRNA even after the treatment, suggesting the cytokine environment still maintains the Th1 immune cell environment. The result is in line with the previous report (8), and it is not surprising that the CM cytokine environment is Th1-dominant even after cholera toxin and Ascaris administration because most NHP species maintains Th1 environment (5). We also tried to detect CM IgE, by using ELISA method and mass spectrometry in CM plasma, but no detectable IgE was observed, while CM IgE gene has not been identified yet (23).…”

Section: Semi-quantitative Rt-pcrsupporting

confidence: 82%

“…We reported that CM possesses CD4T cells, CD8T cells and B cells in the similar ratio in PBMC and the T cells express CD25 and various cytokines after the activation, similarly to the cells of mouse and human (8). This evidence indicates that the CM is useful as a model to analyze human immune disease.…”

Section: Introductionmentioning

confidence: 72%

“…Callithrix jacchus (common marmoset; CM) is a new world monkey (7), for which genome sequence analysis and transgenic animals have been established (8,9). We reported that CM possesses CD4T cells, CD8T cells and B cells in the similar ratio in PBMC and the T cells express CD25 and various cytokines after the activation, similarly to the cells of mouse and human (8).…”

Section: Introductionmentioning

confidence: 97%

“…According to our results shown in Figure 2C and as Fujii et al reported, TGF-β mRNA level was high and those of IL-6 and IL-4 were low in CMs without treatment. IL-17 was expressed at a detectable level (8,15). The cytokine environment observed in CM PBMCs was predicted not to be adequate to Th2 development, but adequate to Treg and IL-27 enhanced IL-10 secreting cell differentiation.…”

Section: Semi-quantitative Rt-pcrmentioning

confidence: 99%

“…The mAbs used were as follows: anti-human CD3-Peridinin chlorophyll protein-cyanine5.5 (PerCPCy5.5) (SP34-2), streptavidin-PE and allophycocyanin (APC)-labeled streptavidin were purchased from BD Biosciences (NJ, USA). Anti-CM CD4 and anti-CM CD25 monoclonal antibodies were prepared previously (8). Cells were subjected to agarose gel electrophoresis.…”

Section: Flow Cytometrymentioning

confidence: 99%

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The response of common marmoset immunity against cedar pollen extract

Kametani

Yamada

Takabayashi

et al. 2018

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Section: Semi-quantitative Rt-pcrsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 97%

Section: Semi-quantitative Rt-pcrmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

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The response of common marmoset immunity against cedar pollen extract

Kametani

Yamada

Takabayashi

et al. 2018

BST

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Comparative genome analysis of the major histocompatibility complex (MHC) class I B/C segments in primates elucidated by genomic sequencing in common marmoset (Callithrix jacchus)

et al. 2011

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Common marmoset monkeys (Callithrix jacchus) have emerged as important animal models for biomedical research, necessitating a more extensive characterization of their major histocompatibility complex (MHC) region. However, the genomic information of the marmoset MHC (Caja) is still lacking. The MHC-B/C segment represents the most diverse MHC region among primates. Therefore, in this paper, to elucidate the detailed gene organization and evolutionary processes of the Caja class I B (Caja-B) segment, we determined two parts of the Caja-B sequences with 1,079 kb in total, ranging from H6orf15 to BAT1 and compared the structure and phylogeny with that of other primates. This segment contains 54 genes in total, nine Caja-B genes (Caja-B1 to Caja-B9), two MIC genes (MIC1 and MIC2), eight non-MHC genes, two non-coding genes, and 33 non-MHC pseudogenes that have not been observed in other primate MHC-B/C segments. Caja-B3, Caja-B4, and Caja-B7 encode proper MHC class I proteins according to amino acid structural characteristics. Phylogenetic relationships based on 48 MHC-I nucleotide sequences in primates suggested (1) species-specific divergence for Caja, Mamu, and HLA/Patr/Gogo lineages, (2) independent generation of the "seven coding exon" type MHC-B genes in Mamu and HLA/Patr/Gogo lineages from an ancestral "eight coding exon" type MHC-I gene, (3) parallel correlation with the long and short segmental duplication unit length in Caja and Mamu lineages. These findings indicate that the MHC-B/C segment has been under permanent selective pressure in the evolution of primates.

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Creating Genetically Modified Marmosets

Sasaki

2019

The Common Marmoset in Captivity and Biomedical Research

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Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset

Cited by 19 publications

References 42 publications

The response of common marmoset immunity against cedar pollen extract

The response of common marmoset immunity against cedar pollen extract

Comparative genome analysis of the major histocompatibility complex (MHC) class I B/C segments in primates elucidated by genomic sequencing in common marmoset (Callithrix jacchus)

Creating Genetically Modified Marmosets

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