Development of Monoclonal Antibodies that Recognize a Type-2 Specific and a Common Epitope on the Nucleoprotein of Infectious Hematopoietic Necrosis Virus

Ristow, Sandra S.; Arnzen, Jeanene M.

doi:10.1577/1548-8667(1989)001<0119:domatr>2.3.co;2

Cited by 40 publications

(35 citation statements)

References 15 publications

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“…These investigations, reporting the extent of heterogeneity among selected IHNV field isolates, relied upon using biological (Mulcahy et al 1984), serological (Winton et al 1988, Ristow & Arnzen 1989 and biochemical techniques such as electrophoretic mobility of structural proteins (Hsu et al 1986), RNase T1 mapping (Oshima et al 1995), and nucleotide sequencing . These studies have indicated that different strains of IHNV exist and that phenotypic and genetic relatedness generally correlates with geographic origin.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…In order to correlate the phylogenetic types found in this study with an established monoclonal antibody typing system as described in Ristow & Arnzen (1989), 45 (20 M clade and 25 U clade isolates) of the 120 Columbia River basin isolates were tested for reactivity with the the type 2-specific monoclonal antibody 2NH105B (data not shown). This antibody binds to an unidentified epitope in the IHNV N protein of electropherotype 2 IHNV isolates.…”

Section: Correlation With Previous Ihnv Typing Systemsmentioning

confidence: 99%

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Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin

Garver

Troyer²,

Kurath

2003

Dis. Aquat. Org.

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Infectious hematopoietic necrosis virus (IHNV), an aquatic rhabdovirus, causes a highly lethal disease of salmonid fish in North America. To evaluate the genetic diversity of IHNV from throughout the Columbia River basin, excluding the Hagerman Valley, Idaho, the sequences of a 303 nt region of the glycoprotein gene (mid-G) of 120 virus isolates were determined. Sequence comparisons revealed 30 different sequence types, with a maximum nucleotide diversity of 7.3% (22 mismatches) and an intrapopulational nucleotide diversity of 0.018. This indicates that the genetic diversity of IHNV within the Columbia River basin is 3-fold higher than in Alaska, but 2-fold lower than in the Hagerman Valley, Idaho. Phylogenetic analyses separated the Columbia River basin IHNV isolates into 2 major clades, designated U and M. The 2 clades geographically overlapped within the lower Columbia River basin and in the lower Snake River and tributaries, while the upper Columbia River basin had only U clade and the upper Snake River basin had only M clade virus types. These results suggest that there are co-circulating lineages of IHNV present within specific areas of the Columbia River basin. The epidemiological significance of these findings provided insight into viral traffic patterns exhibited by IHNV in the Columbia River basin, with specific relevance to how the Columbia River basin IHNV types were related to those in the Hagerman Valley. These analyses indicate that there have likely been 2 historical events in which Hagerman Valley IHNV types were introduced and became established in the lower Columbia River basin. However, the data also clearly indicates that the Hagerman Valley is not a continuous source of waterborne virus infecting salmonid stocks downstream. KEY WORDS: Columbia River basin · IHNV · Rhabdovirus · Epidemiology · Salmonids Resale or republication not permitted without written consent of the publisherDis Aquat Org 55: [187][188][189][190][191][192][193][194][195][196][197][198][199][200][201][202][203] 2003 ture with chinook salmon and steelhead culture, the occurrence of IHNV in the Columbia River basin became much less apparent. Prior to the late-1970s, IHNV was isolated only sporadically within the Columbia River basin. A survey conducted in 1970 by Amend and Wood for IHNV in Washington salmon indicated that IHNV was not present in stocks of chinook O. tshawytscha or coho O. kisutch, and only a portion of the sockeye salmon were found to be infected (Amend & Wood 1972). In Oregon, during the 1970s, IHN disease was observed in stocks of rainbow trout, steelhead trout, chinook salmon and kokanee salmon, but the virus was endemic to only 2 facilities (Mulcahy et al. 1980, Groberg & Fryer 1983, Groberg et al. 1983. In 1977, an area of the Columbia River basin located in Hagerman Valley, Idaho, began to experience high mortalities at rainbow trout aquaculture facilities (Busch 1983). The virus spread over the next 3 years to become endemic to the Hagerman Valley. During the subsequent years of 1980 ...

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Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Section: Correlation With Previous Ihnv Typing Systemsmentioning

confidence: 99%

Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin

Garver

Troyer²,

Kurath

2003

Dis. Aquat. Org.

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“…The IHNV genome contains six genes in the order 39-N-P-M-G-NV-L-59, representing the nucleocapsid, phosphoprotein, matrix protein, glycoprotein, non-virion protein and polymerase protein genes, respectively (Kurath et al, 1985;Morzunov et al, 1995). Phenotypic and genetic diversity among IHNV isolates from different geographical sources have been investigated since the 1980s using various methods, including protein electropherotyping (Hsu et al, 1986), monoclonal antibody reactivity (Ristow & Arnzen, 1989;Ristow & Arnzen de Avila, 1991;Winton et al, 1988;LaPatra et al, 1994) and RNase T1 fingerprinting (Oshima et al, 1995). Phylogenetic analyses of the complete glycoprotein and non-virion protein gene sequences of twelve IHNV isolates confirmed the earlier studies in concluding that IHNV genetic types correlate with geography .…”

Section: Introductionmentioning

confidence: 99%

Phylogeography of infectious haematopoietic necrosis virus in North America

Kurath

Garver

Troyer

et al. 2003

Journal of General Virology

195

209

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Infectious hematopoietic necrosis virus (IHNV) is a rhabdoviral pathogen that infects wild and cultured salmonid fish throughout the Pacific Northwest of North America. IHNV causes severe epidemics in young fish and can cause disease or occur asymptomatically in adults. In a broad survey of 323 IHNV field isolates, sequence analysis of a 303 nucleotide variable region within the glycoprotein gene revealed a maximum nucleotide diversity of 8?6 %, indicating low genetic diversity overall for this virus. Phylogenetic analysis revealed three major virus genogroups, designated U, M and L, which varied in topography and geographical range. Intragenogroup genetic diversity measures indicated that the M genogroup had three-to fourfold more diversity than the other genogroups and suggested relatively rapid evolution of the M genogroup and stasis within the U genogroup. We speculate that factors influencing IHNV evolution may have included ocean migration ranges of their salmonid host populations and anthropogenic effects associated with fish culture. INTRODUCTIONInfectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that causes acute, systemic disease in salmonid fish and also occurs in asymptomatic fish hosts. The virus is currently endemic throughout the Pacific Northwest of North America, with a contiguous range extending from Alaska to California and inland to Idaho. Within this geographical area the host range of IHNV includes five species of Pacific salmon, Atlantic salmon and several trout species (Wolf, 1988;Bootland & Leong, 1999). The first reported epidemics of IHNV occurred in sockeye salmon (Oncorhynchus nerka) fry at Washington and Oregon fish hatcheries during the 1950s (Rucker et al., 1953;Guenther et al., 1959;Wingfield et al., 1969). Surveys indicated that IHNV was endemic in sockeye throughout Alaska by 1974(Grischkowsky & Amend, 1976, but the virus was not widespread in Washington and Oregon through the 1970s (Amend & Wood, 1972; Mulcahy et al., 1980;Pilcher & Fryer, 1980). Subsequently, two virus emergence events occurred in which IHNV became endemic in rainbow trout (O. mykiss) throughout the Hagerman Valley trout farming industry in southern Idaho between 1977(Busch, 1983 and in salmonids of the middle and lower Columbia River basin in the early 1980s (Groberg, 1983;Groberg & Fryer, 1983). In addition to cultured fish, IHNV is endemic in many wild salmonid stocks in the Pacific Northwest (Bootland & Leong, 1999).Due to the extensive economic losses caused by IHNV in fish culture facilities, the virus has been well characterized in biological, immunological and molecular biological studies (for reviews, see Wolf, 1988;Bootland & Leong, 1999). IHNV is the type species of the genus Novirhabdovirus, within the family Rhabdoviridae. Similar to other rhabdoviruses, IHNV has a linear single-stranded, negative-sense RNA genome of approximately 11 000 nucleotides. The IHNV genome contains six genes in the order 39-N-P-M-G-NV-L-59, representing the nucleocapsid, phosphoprotein, matrix protein, glyco...

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“…Monoclonal antibodies: Monoclonal antibodies to the glycoproteins and nucleoproteins of a number of different isolates of IHN virus were produced as descri.bed previously (Ristow & Arnzen 1989, &stow & Arnzen de Avila 1991. In this study, 6 of these rnonoclonal antibodies were utilized in indirect fluorescence to screen the French isolates: lNDWl4D (produced by an immunization with the nucleoprotein of a Dworshak isolate 2 and found to react with all 28 North American isolates tested); 1NDWl9I (also produced from an immunization to Dworshak isolate nucleoprotein, but not reacting with all North American isolates); 1NC027G (an anti-nucleoprotein antibody produced from a fusion against a Coleman isolate from chinook salmon Oncorhynchus tshawytscha); 3GH127B and 1NH139P (anti-glycoprotein and anti-nucleoprotein antibodies produced in a fusion in which the glycoprotein and the nucleoprotein of Hagerman virus, isolate 039-82SR, were the respective immunogens); and 2NH105B (an anti-nucleoprotein antibody made by immunization with the nucleoprotein of Hagerman virus 039-82SR), which recognizes electrophoretic type 2 IHNV (Hsu et al 1986, Ristow & Arnzen 1989.…”

Section: Infectious Haematopoietic Necrosis Virus (Ihnv) Ismentioning

confidence: 99%

Typing of French isolates of infectious haematopoietic necrosis virus (IHNV) with monoclonal antibodies using indirect immunofluorescence

Danton¹,

Ristow²,

Hattenberger-Baudouy³

et al. 1994

Dis. Aquat. Org.

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Following the first isolation of infectious haematopoietic necrosis virus (IHNV) in France, many other isolates of the virus have been recovered from rainbow trout populations. Of the 27 isolates typed using an indirect irnrnunofluorescence (IIF) test with mouse monoclonal antibodies (MAb), only 5 of the French isolates were recognized by MAb 1NDW14D, previously considered to be a universal IF reagent for IHNV. In contrast, all 27 isolates shared the nucleocapsld epitope identified by MAb 2NH105B, currently thought to be speclfic for IHNV of electropherotype 2, although the French isolates did not appear to belong to this electropherotype. Considerable variation was noted among the isolates in other nucleocapsid epitopes, suggesting that some of the isolates have been present among rainbow trout, in Europe or elsewhere, for sufficient time to evolve separately. Practically speaking, a mult~valent IHNV reagent for IIF based upon MAbs should encompass both MAbs lNDW14D and 2NH105B, especially if intended for European use.

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Development of Monoclonal Antibodies that Recognize a Type-2 Specific and a Common Epitope on the Nucleoprotein of Infectious Hematopoietic Necrosis Virus

Cited by 40 publications

References 15 publications

Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin

Two distinct phylogenetic clades of infectious hematopoietic necrosis virus overlap within the Columbia River basin

Phylogeography of infectious haematopoietic necrosis virus in North America

Typing of French isolates of infectious haematopoietic necrosis virus (IHNV) with monoclonal antibodies using indirect immunofluorescence

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