Development of ‘multiresistance rice’ by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system

Li, Chuanxu; Zhang, Jianguo; Ren, Zhiyong; Rong, Xie; Yin, Changxi; Ma, Weihua; Zhou, Fei; Chen, Hao

doi:10.1002/ps.6178

Cited by 26 publications

(19 citation statements)

References 90 publications

(152 reference statements)

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“…These wheat lines showed very high resistance to Pgt ( Luo et al., 2021b ), suggesting that a novel and superior wheat germplasm could be obtained using synthetic biology. In rice, a highly efficient transgene stacking system was established to assemble six genes (the synthetic glyphosate-tolerance gene I. variabili s -EPSPS∗ , the lepidopteran pest resistance gene Cry1C∗ , the brown planthopper resistance genes Bph14∗ and OsLecRK1∗ , the bacterial blight resistance gene Xa23∗ , and the rice blast resistance gene Pi9∗ ) onto a transformable artificial chromosome vector, “multi-resistance rice” ( Li et al., 2020g ). Rice plants with improved pest and disease resistance were successfully generated.…”

Section: Beyond Genome Editingmentioning

confidence: 99%

Present and future prospects for wheat improvement through genome editing and advanced technologies

Zhang

et al. 2021

Plant Communications

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Wheat ( Triticum aestivum , 2 n = 6 x = 42, AABBDD) is one of the most important staple food crops in the world. Despite the fact that wheat production has significantly increased over the past decades, future wheat production will face unprecedented challenges from global climate change, increasing world population, and water shortages in arid and semi-arid lands. Furthermore, excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration. To ensure global food and ecosystem security, it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies. However, the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement, thus impeding the breeding of elite wheat cultivars. In this review, we first introduce state-of-the-art genome-editing technologies in crop plants, especially wheat, for both functional genomics and genetic improvement. We then outline applications of other technologies, such as GWAS, high-throughput genotyping and phenotyping, speed breeding, and synthetic biology, in wheat. Finally, we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies. We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement of wheat for sustainable global production.

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Section: Beyond Genome Editingmentioning

confidence: 99%

Present and future prospects for wheat improvement through genome editing and advanced technologies

Zhang

et al. 2021

Plant Communications

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“…As a staple food for more than one-half of the world’s population, rice is frequently exposed to the threats of bacterial diseases. , Among these diseases, bacterial leaf streak (BLS) and bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzicola ( Xoc ) and Xanthomonas oryzae pv.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Antibacterial Activity, and Mechanisms of Novel 6-Sulfonyl-1,2,4-triazolo[3,4-b][1,3,4]thiadiazole Derivatives

Shi

Chen

et al. 2021

J. Agric. Food Chem.

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A series of novel 6-sulfonyl-1,2,4-triazolo [3,4-b][1,3,4]thiadiazole derivatives were designed and synthesized. CoMFA models were established to analyze the quantitative structure−activity relationships on the basis of the EC 50 values of the compounds. The models were used to design and synthesize compounds 32 and 33 with higher activities. The EC 50 values of compound 33 against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) were 0.59 and 1.63 mg/L, respectively, which were higher than those of thiodiazole copper (90.43 and 97.93 mg/L) and bismerthiazol (68.37 and 75.59 mg/ L). Moreover, protective activities of compound 33 against bacterial leaf streak (BLS) and bacterial leaf blight (BLB) were 49.65% and 49.42%, respectively, which were superior to those of thiodiazole copper (44.28% and 41.51%) and bismerthiazol (38.89% and 40.09%). Protective activity of compound 33 against BLS was closely related to the improvement of defense-related enzyme activities, chlorophyll content, and photosynthesis activation. This is consistent with the upregulated expression of defense responses and photosynthesis-related proteins.

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“…Furthermore, stacking Pi54 and Pi54rh showed a higher level of resistance to a set of highly virulent M. oryzae isolates collected from different rice-growing regions (Kumari et al 2017 ). Pyramiding of Pi9 together with multiple genes resistant to brown planthopper, bacterial blight and lepidopteran pest in rice showed significantly higher yield with imparted multiple resistances, including the blast (Li et al 2021 ). Here, we report pyramiding of three novel Pi genes Pib , Pi25 and Pi54 in rice via an Agrobacterium -mediated transgenic approach, providing more information for effectively breeding rice cultivars with durable blast resistance.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Evaluation of Transgenic Rice Pyramided with the Pi Genes Pib, Pi25 and Pi54

Xiang

et al. 2021

Rice

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Background Emergence of new pathogen strains of Magnaporthe oryzae is a major reason for recurrent failure of the resistance mediated by a single resistance gene (Pi) in rice. Stacking various Pi genes in the genome through marker-assisted selection is thus an effective strategy in rice breeding for achieving durable resistance against the pathogen. However, the effect of pyramiding of multiple Pi genes using transgenesis still remains largely unknown. Results Three Pi genes Pib, Pi25 and Pi54 were transferred together into two rice varieties, the indica variety Kasalath and the japonica variety Zhenghan 10. Transgenic plants of both Kasalath and Zhenghan 10 expressing the Pi transgenes showed imparted pathogen resistance. All the transgenic lines of both cultivars also exhibited shorter growth periods with flowering 2–4 days early, and shorter plant heights with smaller panicle. Thus, pyramiding of the Pi genes resulted in reduced grain yields in both rice cultivars. However, tiller numbers and grain weight were generally similar between the pyramided lines and corresponding parents. A global analysis of gene expression by RNA-Seq suggested that both enhancement and, to a lesser extent, inhibition of gene transcription occurred in the pyramided plants. A total of 264 and 544 differentially expressed genes (DEGs) were identified in Kasalath and Zhenghan 10, respectively. Analysis of the DEGs suggested that presence of the Pi transgenes did not alter gene expression only related to disease resistance, but also impacted many gene transcriptions in the pathways for plant growth and development, in which several were common for both Kasalath and Zhenghan 10. Conclusion Pyramiding of the Pi genes Pib, Pi25 and Pi54 via transgenesis is a potentially promising approach for improving rice resistance to the pathogen Magnaporthe oryzae. However, pleiotropic effects of the Pi genes could potentially result in yield loss. These findings support the idea that immunity is often associated with yield penalties. Rational combination of the Pi genes based on the genetic background may be important to balance yield and disease resistance.

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Development of ‘multiresistance rice’ by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system

Cited by 26 publications

References 90 publications

Present and future prospects for wheat improvement through genome editing and advanced technologies

Present and future prospects for wheat improvement through genome editing and advanced technologies

Synthesis, Antibacterial Activity, and Mechanisms of Novel 6-Sulfonyl-1,2,4-triazolo[3,4-b][1,3,4]thiadiazole Derivatives

Characterization and Evaluation of Transgenic Rice Pyramided with the Pi Genes Pib, Pi25 and Pi54

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