Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom

Bailon, Henri; Coronel, Verónica Olga Yaniro; Rey, Omar A.; Alave, Elizabeth Gaby Colque; Duran, Walter Jhon Leiva; Rojas, Carlos Padilla; Arevalo, Harrison Montejo; Neyra, David García; Pérez, Marco Galarza; Bonilla, César; Tintaya, B.; Ricciardi, Giulia; Smiejkowska, Natalia; Romão, Ema; Vincke, Cécile; Lévano, Juan; Celys, Mary; Lomonte, Bruno; Muyldermans, Serge

doi:10.3389/fimmu.2020.00655

Cited by 41 publications

(21 citation statements)

References 35 publications

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“…Anti-scorpions toxin nanobody was able to protect mice more quickly than PAS therapy from Androctonus australis hector (Aah) scorpions [ 88 ]. In another study, despite the nanobody mixture failed to stop the venom lethality, the results proved the efficacy and usefulness of VHH developed against Bothrops atrox snake venom [ 93 ], which highlights the potential of VHH as anti-toxin agents.…”

Section: Nanobody and Scfv In Applicationmentioning

confidence: 93%

A comprehensive comparison between camelid nanobodies and single chain variable fragments

et al. 2021

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By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications.

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Section: Nanobody and Scfv In Applicationmentioning

confidence: 93%

A comprehensive comparison between camelid nanobodies and single chain variable fragments

et al. 2021

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“…Nanobodies are proposed to be used as antivenoms, showing notable advantages such as rapid diffusion, high-biodistribution due to their small size and no Fc region (responsible for adverse reactions in antivenom therapy) in their structure ( 30 ). A recent study demonstrated their efficacy in neutralizing both local tissue hemorrhage and myonecrosis in mice after SBE, when applied a mixture of selected specific nanobodies ( 31 ), showing the potential of this therapy over Fab or IgG- based antivenoms in resolving local damage.…”

Section: Snakebite Envenoming (Sbe) Relevancementioning

confidence: 99%

Mesenchymal Stromal Cell-Based Therapies as Promising Treatments for Muscle Regeneration After Snakebite Envenoming

et al. 2021

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Snakebite envenoming is a global neglected disease with an incidence of up to 2.7 million new cases every year. Although antivenoms are so-far the most effective treatment to reverse the acute systemic effects induced by snakebite envenoming, they have a limited therapeutic potential, being unable to completely neutralize the local venom effects. Local damage, such as dermonecrosis and myonecrosis, can lead to permanent sequelae with physical, social, and psychological implications. The strong inflammatory process induced by snake venoms is associated with poor tissue regeneration, in particular the lack of or reduced skeletal muscle regeneration. Mesenchymal stromal cells (MSCs)-based therapies have shown both anti-inflammatory and pro-regenerative properties. We postulate that using allogeneic MSCs or their cell-free products can induce skeletal muscle regeneration in snakebite victims, improving all the three steps of the skeletal muscle regeneration process, mainly by anti-inflammatory activity, paracrine effects, neovascularization induction, and inhibition of tissue damage, instrumental for microenvironment remodeling and regeneration. Since snakebite envenoming occurs mainly in areas with poor healthcare, we enlist the principles and potential of MSCs-based therapies and discuss regulatory issues, good manufacturing practices, transportation, storage, and related-procedures that could allow the administration of these therapies, looking forward to a safe and cost-effective treatment for a so far unsolved and neglected health problem.

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“…Adicionalmente, los Nac también se han desarrollado como antivenenos, capaces de neutralizar o disminuir los efectos de las mordeduras de serpientes (Bailon et al, 2020) o de las picaduras de los arácnidos (Tabla II). En la Figura 4, se observa su potencial aplicación de forma esquemática.…”

Section: Nanoanticuerpos Para La Industriaunclassified

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

et al. 2021

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Los anticuerpos monoclonales son una de las herramientas más revolucionarias en el área de la biomedicina por tener aplicaciones inmunoterapéuticas e inmunodiagnósticas. Sin embargo, el rápido avance tecnológico que demandan estas áreas genera la exploración de nuevas biomoléculas. El descubrimiento de anticuerpos compuestos únicamente por cadenas pesadas, presentes de forma natural en el suero de los camélidos y en algunas especies de tiburón, son motivo de estudio desde las últimas décadas como una alternativa a los anticuerpos convencionales. Estos poseen una región de reconocimiento antigénico, que consiste en un dominio variable por cada cadena, conocidos como anticuerpos de un solo dominio o nanoanticuerpos. Estas biomoléculas se caracterizan por tener un tamaño reducido, alta especificidad, estabilidad y bajo costo en su producción; propiedades que las convierten en una herramienta altamente versátil. En la presente revisión se abordarán aspectos relevantes de los nanoanticuerpos, como su descubrimiento, características estructurales, desarrollo en el campo de la biotecnología y su potencial de aplicación en enfermedades como el cáncer y en la identificación de microorganismos.

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Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom

Cited by 41 publications

References 35 publications

A comprehensive comparison between camelid nanobodies and single chain variable fragments

A comprehensive comparison between camelid nanobodies and single chain variable fragments

Mesenchymal Stromal Cell-Based Therapies as Promising Treatments for Muscle Regeneration After Snakebite Envenoming

Nanoanticuerpos: desarrollo biotecnológico y aplicaciones

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