Development of Nanocellulose-Based Bioinks for 3D Bioprinting of Soft Tissue

Gatenholm, Paul; Ávila, Héctor Martínez; Amoroso, Matteo; Karabulut, Erdem; Kölby, Lars; Markstedt, Kajsa; Gatenholm, Erik; Henriksson, Ida

doi:10.1007/978-3-319-40498-1_14-1

Cited by 11 publications

(5 citation statements)

References 78 publications

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“…Indeed, small diameter nozzles combined with high extrusion pressures significantly compromised the cells. Currently, the NFC/ALG composite ink formulation has been commercialized with the brand named CELLINK ® and has been tested in vitro and in vivo with human chondrocytes and iPSC cells derived from chondrocytes [ 41 , 42 ]. Henriksson et al [ 41 ] successfully formulated a bioink composed of NC and HA (CELLINK-H) for 3D bioprinting in adipose tissue engineering, demonstrating its advantage above the conventional 2D culture system in terms of adipogenic differentiation potential and phenotype maintenance.…”

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

“…Henriksson et al [ 41 ] successfully formulated a bioink composed of NC and HA (CELLINK-H) for 3D bioprinting in adipose tissue engineering, demonstrating its advantage above the conventional 2D culture system in terms of adipogenic differentiation potential and phenotype maintenance. Moreover, recently, a clinical study on CELLINK ® bioink has also been performed in mice [ 42 ]. Results have shown that the 3D-bioprinted scaffolds have excellent structural integrity, shape fidelity, and suitable mechanical behavior after 60 days of implantation (with an increase in the compressive stress from 14.9 to 46 kPa).…”

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

“…The explanted constructs showed a good proliferation ability and ECM deposition after 60 days. The bioprinted cell-laden NFC/ALG scaffold was also studied for its in vivo chondrogenesis potential in a co-culture (hNCs/human bone marrow-derived MSCs (hBMSCs)) system, reporting satisfactory results [ 42 ]. An MSC-containing NC/ALG/HA formulation was demonstrated to be an excellent bioink for the development of 3D-bioprinted scaffolds for CTE by Lafuente-Merchan et al [ 38 ].…”

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

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Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

Szychlinska

Bucchieri

Fucarino

et al. 2022

JFB

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In regenerative medicine and tissue engineering, the possibility to: (I) customize the shape and size of scaffolds, (II) develop highly mimicked tissues with a precise digital control, (III) manufacture complex structures and (IV) reduce the wastes related to the production process, are the main advantages of additive manufacturing technologies such as three-dimensional (3D) bioprinting. Specifically, this technique, which uses suitable hydrogel-based bioinks, enriched with cells and/or growth factors, has received significant consideration, especially in cartilage tissue engineering (CTE). In this field of interest, it may allow mimicking the complex native zonal hyaline cartilage organization by further enhancing its biological cues. However, there are still some limitations that need to be overcome before 3D bioprinting may be globally used for scaffolds’ development and their clinical translation. One of them is represented by the poor availability of appropriate, biocompatible and eco-friendly biomaterials, which should present a series of specific requirements to be used and transformed into a proper bioink for CTE. In this scenario, considering that, nowadays, the environmental decline is of the highest concerns worldwide, exploring naturally-derived hydrogels has attracted outstanding attention throughout the scientific community. For this reason, a comprehensive review of the naturally-derived hydrogels, commonly employed as bioinks in CTE, was carried out. In particular, the current state of art regarding eco-friendly and natural bioinks’ development for CTE was explored. Overall, this paper gives an overview of 3D bioprinting for CTE to guide future research towards the development of more reliable, customized, eco-friendly and innovative strategies for CTE.

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Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

Section: Naturally-derived Bioinks For 3d Bioprinting Of Cartilage Ti...mentioning

confidence: 99%

See 1 more Smart Citation

Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

Szychlinska

Bucchieri

Fucarino

et al. 2022

JFB

View full text Add to dashboard Cite

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“…[20,21] Numerous studies have successfully incorporated NFC into hydrogel systems as reinforced fiber to enhance mechanical strength and shape integrity. [22][23][24][25][26] Interestingly, alginate-NFC hydrogels were found to induce the expression of cartilage phenotype while having a compressive modulus in the range of 0.53 AE 0.59 MPa after 10 months of implantation in nude mice. [27] Another in vivo investigation using 3D printed alginate-NFC constructs preseeded with human primary nasal chondrocytes (hNCs) reported detection of sulfated glycosaminoglycans (GAGs) and collagen type II (Col II) after 90 days [28] Both studies confirmed that alginate-NFC composites had good structural integrity and can induce cartilage differentiation in vivo.…”

Section: Introductionmentioning

confidence: 99%

Reinforcing Tissue‐Engineered Cartilage: Nanofibrillated Cellulose Enhances Mechanical Properties of Alginate Dialdehyde–Gelatin Hydrogel

Chayanun,

Soufivand,

Faber

et al. 2023

Adv Eng Mater

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Cartilage tissue engineering offers a promising option for treating osteochondral defects. Alginate dialdehyde–gelatin (ADA–GEL) hydrogel has been explored as promising material for soft tissue scaffolds; however, its low stiffness has posed a constraint to load bearing applications. Herein, this limitation is addressed by introducing nanofibrillated cellulose (NFC) into the ADA–GEL matrix. The effect of NFC on the physicochemical properties of hydrogels is evaluated. Fourier transform infrared spectra demonstrate no chemical interaction between NFC and ADA–GEL, while scanning electron microscopy pictures reveal NFC fibers embedded in the hydrogel matrix, thus confirming the fiber‐reinforced composite hypothesis. NFC‐reinforced ADA–GEL (AG‐N) composite hydrogels exhibit increased stiffness, with a maximum compressive effective modulus of 19.6 ± 3.0 kPa at 25% w/w NFC content. ATDC5 cell viability and proliferation as well as chondrogenic differentiation are assessed using immunohistochemical staining for sulfated glycosaminoglycans and collagen type II. A possible application of AG‐N hydrogels as an osteochondral plug is also proposed, with polyetheretherketone as the subchondral bone anchor part. The mechanical properties of the resulting osteochondral device highlight its potential as a promising biomaterial for treatment of osteochondral defects. These findings provide valuable insights into the development of AG‐N hydrogels for load‐bearing tissue engineering applications.

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“…The idea of inkjet-printing NCs as well as nanofibers has been reported to be applied to 3D printers in recent years, − where desired moldings were made by careful viscosity control and combination with other ingredients. These researches are very attractive, but even with 2D inkjet printing, if we can fully exploit the inherent characteristics of NCs, we can make it meaningful by accomplishing fine molding.…”

Section: Introductionmentioning

confidence: 99%

Simple Inkjet Process To Fabricate Microstructures of Chitinous Nanocrystals for Cell Patterning

Suzuki

Teramoto

2017

Biomacromolecules

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Structural polysaccharide nanocrystals (NCs) including cellulose nanocrystal have attracted attention. In order to broaden the range of application of the NCs, we can take advantage of their original characteristics by establishing simple and reasonable processing methods. We here demonstrate a micropatterning of animal cellular adhesion by inkjet printing of aqueous dispersions of cytocompatible chitinous NCs onto cellophane films. We display how to regulate the deposition form and two-dimensional shape of the chitinous NC micromoldings using a research inkjet printer. Adhesive capability of mouse fibroblasts onto the chitinous substrates was greatly improved by alkali deacetylation. The deacetylated products remained rod-like nanostructures, but the original chitin crystal form changed to that of chitosan by an intensive deacetylation. The adhered cells could be recovered glycolytically. The chitinous micropatterning substrates can be utilized for biomedical applications such as controlling of cellular shapes, precise monitoring molecular events in biochemistry, and drug screening.

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Development of Nanocellulose-Based Bioinks for 3D Bioprinting of Soft Tissue

Cited by 11 publications

References 78 publications

Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

Three-Dimensional Bioprinting for Cartilage Tissue Engineering: Insights into Naturally-Derived Bioinks from Land and Marine Sources

Reinforcing Tissue‐Engineered Cartilage: Nanofibrillated Cellulose Enhances Mechanical Properties of Alginate Dialdehyde–Gelatin Hydrogel

Simple Inkjet Process To Fabricate Microstructures of Chitinous Nanocrystals for Cell Patterning

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