Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration

Ahn, Yun Gyong; Seo, Jungju; Shin, Jeoung Hwa; Khim, Jong Seong; Hong, Jongki

doi:10.1016/j.aca.2006.03.010

Cited by 21 publications

(9 citation statements)

References 22 publications

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“…The lipid removal (%) was calculated from the lipid residue after freezing-lipid filtration. The detailed freezing-lipid filtration procedure has been described previously [28][29][30]. Sulfuric acid treatment was performed following EPA method 1614 for comparison with the freezing-lipid filtration method.…”

Section: Sample Extraction and Delipidationmentioning

confidence: 99%

“…Recent reports have recommended using the freezing-lipid filtration method to eliminate large quantities of lipids from biological samples for an efficient cleanup. This method has been used for the successful determination of chlorinated pesticides, anabolic steroids, synthetic hormones and PCDD/Fs in biological matrices [27][28][29]. Most lipids can be extracted from biological samples through freezing-lipid filtration.…”

Section: Introductionmentioning

confidence: 99%

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Development of a cleanup method for polybrominated diphenyl ether (PBDE) in fish by freezing-lipid filtration

Shin

Boo

Bang

et al. 2012

Eur Food Res Technol

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A new cleanup method for the determination of brominated flame retardants with an emphasis on polybrominated diphenyl ethers (PBDEs) has been developed for fish tissue sample. This method effectively reduces the sample pretreatment time, labor and required less solvent quantities relative to conventional methods. Freezing-lipid filtration procedure removes approximately 90 % of the lipids in the extract without any significant loss of the PBDEs. A multilayered silica gel column was used for further cleanup of the extracts after freezing-lipid filtration. Multilayered silica gel column chromatography eliminated most of the co-extracted interferences, such as residual lipids and fatty acids. The extracts were analyzed after cleanup by high-resolution gas chromatography/highresolution mass spectrometry using the isotope dilution method. Tissue samples with 1.6-8.0 and 8.0-40 ppb of PBDE were analyzed using both the sulfuric acid treatment and freezing-lipid filtration cleanup methods in order to evaluate the method performance. Sulfuric acid treatment did not detect 2,4-DiBDE, whereas freezing-lipid filtration detected 2,4-DiBDE but at 50 % recovery. To compare the method, WMF-01 was analyzed via both the sulfuric acid treatment and freezing-lipid filtration cleanup.

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Section: Sample Extraction and Delipidationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a cleanup method for polybrominated diphenyl ether (PBDE) in fish by freezing-lipid filtration

Shin

Boo

Bang

et al. 2012

Eur Food Res Technol

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“…Haglund et al (2007), Spinnel, Danielsson, and Haglund (2008), Wiberg et al (2007), and Nording et al (2005), have developed procedures using a modified ASE cell to allow the sample matrix to sit above an in situ carbon column so that extraction and sample cleanup can be carried out in the same cell. Ahn et al (2006) have developed a freezing-lipid filtration procedure to cleanup fish extracts.…”

Section: Extract Cleanupmentioning

confidence: 99%

The analysis of dioxins and related compounds

Reiner

2009

Mass Spectrometry Reviews

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The analysis of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, polychlorinated biphenyls, and other related compounds requires complex sample preparation and analytical procedures using highly sensitive and selective state-of-the-art instrumentation to meet very stringent data quality objectives. The analytical procedures (extraction, sample preparation), instrumentation (chromatographic separation and detection by mass spectrometry) and screening techniques for the determination of dioxins, furans, dioxin-like polychlorinated biphenyls and related compounds with a focus on new approaches and alternate techniques to standard regulatory methods are reviewed.

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“…Recently, procedures for GC analysis of fatty foods have been used freezing to precipitate fat after solvent extraction . In freezing step, fat becomes frozen and can be easily removed by centrifugation or filtration . Protocols using freezing‐lipid filtration (FLF) have been applied for clean up samples of fish and soybean oil with the efficiency of the fat removal ranged around 90% .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of lipid removal from animal fats for the determination of organochlorine, pesticides, and polychlorinated biphenyl indicators by gas chromatography with electron capture detector

Andrade

Sacheto

Hoff

et al. 2012

J of Separation Science

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For cleanup of animal fat before GC analysis of organochlorine pesticides and polychlorinated biphenyls, freezing-lipid filtration with solid-phase extraction and matrix solid-phase dispersion were evaluated to replace the official Brazilian methodology that uses preparative alumina column chromatography. General drawbacks associated with this last technique, such as the use of large amounts of solvent, laborious and time-consuming procedure could be avoided by using these alternative approaches. Experiments were carried out to study the performance by using different combinations of sorbents and elution solvents. Efficiency of alternative extraction methods in terms of fat removal and recovery capability was monitored by gravimetry, TLC, and GC with electron capture detection. Freezing-lipid filtration with solid-phase extraction afforded better clean up efficiency with recoveries in a range of 54.5 to 103.6% with the relative standard deviation of less than 10% for all compounds under investigation.

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Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration

Cited by 21 publications

References 22 publications

Development of a cleanup method for polybrominated diphenyl ether (PBDE) in fish by freezing-lipid filtration

Development of a cleanup method for polybrominated diphenyl ether (PBDE) in fish by freezing-lipid filtration

The analysis of dioxins and related compounds

Evaluation of lipid removal from animal fats for the determination of organochlorine, pesticides, and polychlorinated biphenyl indicators by gas chromatography with electron capture detector

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