2008
DOI: 10.1016/j.fgb.2008.04.011
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Development of new tools for studying gene function in fungi based on the Gateway system

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Cited by 67 publications
(55 citation statements)
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“…This approach has been widely used in many fungal species (45,57,58,65). To facilitate the plasmid construction, several vectors have been developed, including a pSilent-1 vector that allows high-throughput RNA silencing (50,80). The use of an inducible promoter to control the expression of the hairpin RNA offers significant advantages over constitutive promoters, especially when essential genes are the targets.…”
Section: Applications Of Rnai In Fungimentioning
confidence: 99%
“…This approach has been widely used in many fungal species (45,57,58,65). To facilitate the plasmid construction, several vectors have been developed, including a pSilent-1 vector that allows high-throughput RNA silencing (50,80). The use of an inducible promoter to control the expression of the hairpin RNA offers significant advantages over constitutive promoters, especially when essential genes are the targets.…”
Section: Applications Of Rnai In Fungimentioning
confidence: 99%
“…The ongoing optimization of existing RNAi systems demonstrates the importance of such tools in deciphering gene functions, particularly when gene deletions by homologous recombination occur only at very low frequencies (Caracuel-Rios & Talbot, 2008;Nguyen et al, 2008;Oliveira et al, 2008;Shafran et al, 2008). Several RNAi approaches have been successfully performed using vectors that contain intron or spacer sequences between two inversely oriented target gene fragments to express dsRNA with a hairpin structure (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…Gene silencing by RNA interference (RNAi) is a common method for down-regulating genes in a variety of model systems [130,131], and the recent demonstration of RNAi mechanisms in the model white-rot fungus Phanerochaete chrysosporium [132] suggests that RNAi could be used for targeted downregulation of specific genes in species that are useful for biological pretreatment. The availability of convenient gene silencing transformation vectors for ascomycetes such as pSilent [133] and pTroya [134] as well as pHg/pSILBAγ for basidiomycetes [135] will greatly facilitate the investigation of gene function and may also result in the development of modified strains featuring enhanced properties for biological pretreatment of lignocellulosic substrates for biofuels production.…”
Section: Exploiting Fungal Mutants For Biological Pretreatmentmentioning
confidence: 99%