Development of new-type rapid analysis technology of polychlorinated biphenyls by using liquid chromatographic clean-up material (polyvinyl alcohol gel)

Sawatsubashi, Tetsuya; Tsukahara, Chisato; Baba, Keigo; Ohi, Etsumasa; Shinoda, Akiko; Miura, Norio

doi:10.1016/j.chroma.2007.11.014

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…There is much interest in environmental contamination from persistent organic pollutants (POPs) due to their pervasiveness in the environment. Studies on the prevalence , and remediation − of molecules such as polybrominated biphenyls (PBBs) and polychlorinated biphenyls (PCBs) provide evidence concerning the scope of the POP problem. Several studies on PBBs and PCBs ,− document their environmental persistence and propagation in fish and river sediments.…”

Section: Introductionmentioning

confidence: 99%

PCB Association with Model Phospholipid Bilayers

Campbell

Granick

et al. 2008

Environ. Sci. Technol.

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We compare the association of an ortho-substituted and a planar PCB (polychlorinated biphenyls PCB-52 and PCB-77, respectively) with single-component phospholipid bilayers terminated with phosphocholine headgroups. First, fluorescence correlation spectroscopy (FCS) studies of diffusion on supported fluid-phase DLPC show that the ortho-substituted PCB diffuses more slowly, indicating either complex formation or obstructed diffusion. Differential scanning calorimetry (DSC) of vesicles formed from DMPC shows that the gel-to-fluid phase transition temperature is lower for vesicles containing this ortho-substituted PCB. Atomic force microscopy (AFM) shows that, whereas supported bilayers of DMPC containing this ortho-substituted PCB display two melting points, bilayers containing the coplanar PCB display just a single melting point. A model is proposed in which the ortho-substituted PCB resides within the lipid tails of these phospholipid bilayers but the coplanar PCB associates preferentially with the headgroups. This model is consistent with the known membrane disruptive ability of the ortho substituted isomer.

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Section: Introductionmentioning

confidence: 99%

PCB Association with Model Phospholipid Bilayers

Campbell

Granick

et al. 2008

Environ. Sci. Technol.

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“…Because of the concern of possible PCB contamination in a large number of transformers, PCB contamination in every transformer should be tested. However, typical analytical methods using gas chromatography (GC) coupled with either mass spectrometry (MS) or electron capture detection [4][5][6][7] are time consuming and expensive. In the field of medicine, screening with immunoassays is often used, because of its cost-and time-efficiency.…”

Section: Introductionmentioning

confidence: 99%

Screening of polychlorinated biphenyls in insulating oil using a microfluidic based pretreatment and immunoassay

Aota

Date

Terakado

et al. 2015

Analytical Chemistry Research

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a b s t r a c tPolychlorinated biphenyls (PCBs) are persistent organic pollutants in insulating oil of a large number of transformers. A rapid and economical analytical method to detect PCB contamination is still required. To address this issue, we propose here the first microfluidic screening method for PCB contamination in insulating oil. The insulating oil was pretreated using a multilayer capillary column and a microfluidic liquid-liquid partitioning. PCBs in the pretreated oil were measured using a microfluidic kinetic exclusion assay. In order to detect PCBs with high sensitivity, conditions of the microfluidic kinetic exclusion assay were optimized. Measurements were rapidly completed (within 43 min). The measurement range was estimated to be 0.26-3.3 mg/kg defined as the relative absorbance from 20% to 80%. The screening performance (false positive and false negative rates) was tested on fifty real oil samples; results about these tests were discussed in detail, especially suitable cutoff by comparing with the data analyzed using highresolution-gas-chromatography/high-resolution-mass-spectrometry. Finally, the screening performance was confirmed using our proposed stochastic screening model. A cutoff of 0.3 to judge as positive is suitable considering the risk of the PCB release into the environment.

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“…[4][5][6][7] Although their high sensitivity and high accuracy are attractive, these methods are time consuming and costly. Immunoassays for rapid and low-cost screening of PCBs in oil have been proposed.…”

Section: Introductionmentioning

confidence: 99%