Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering

Pattanashetti, Nandini A.; Viana, Tânia; Alves, Nuno; Mitchell, Geoffrey R.

doi:10.1007/s10965-020-02053-0

Cited by 26 publications

(13 citation statements)

References 32 publications

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“…Overall, PCL/HA scaffolds outperformed the PCL MB scaffolds in terms of cellular viability with higher HA content presenting increasing viability outcomes. Similar results have been previously reported by other groups [7,26,[41][42][43][44][45][46]. Regarding blending techniques, differences between the respective SC and MB groups are not statistically significant, with both MB and SC groups presenting positive hDPSCs viability outcomes.…”

Section: Discussionsupporting

confidence: 89%

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Biscaia¹,

Branquinho²,

Alvites³

et al. 2022

Preprint

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Bone tissue engineering has been developed in the past decades, with the engineering of bone substitutes on the vanguard of this regenerative approach. PCL based scaffolds are fairly applied for bone regeneration, and several composites have been incorporated, as to improve the devices’ mechanical properties and tissue ingrowth. In this study, HA was incorporated on PCL based scaffolds with two different proportions, 80:20 and 60:40. Devices were produced with two different techniques, SC and MB, and further investigated with regards to their mechanical characteristics and in vitro cytocompatibility. Results show the MB devices to present more promising mechanical properties, along with the incorporation of HA. The latter is also related to an increase in osteogenic activity and promotion. Overall, this study suggest PCL:HA scaffolds to be promising candidates for bone tissue engineering, particularly when produced by the MB method.

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Section: Discussionsupporting

confidence: 89%

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Biscaia¹,

Branquinho²,

Alvites³

et al. 2022

Preprint

Self Cite

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“…To determine the amount of β‐TCP loaded in the fibers, the thermogravimetric analysis (TGA, ASTM E‐1131‐14) was run. The percentage of the final weight remaining after the complete removal of the polymer in up to 600°C showed the amount of the loaded β‐TCP nanopowder 25,26 …”

Section: Methodsmentioning

confidence: 99%

“…The percentage of the final weight remaining after the complete removal of the polymer in up to 600 C showed the amount of the loaded β-TCP nanopowder. 25,26 2.3.3 | Crystallinity X-ray diffractometer (XRD, UNISANTIS, XMD-300, Swiss) at 45 kV and 0.8 mA under Cu Kα irradiation was employed to analyze the structural characterizations.…”

Section: Thermogravimetric Analysismentioning

confidence: 99%

Preparation and evaluation of bioactive bilayer composite membrane PHB/β‐TCP with ciprofloxacin and vitamin D3 delivery for regenerative damaged tissue in periodontal disease

Tayebi

Parham

Ahangar

et al. 2021

J of Applied Polymer Sci

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Periodontal tissue engineering has made a tremendous change in the treatment of periodontal disease. In this study, electrophoresed poly-hydroxy-butyrate (PHB) scaffold, beta-three calcium phosphate (β-TCP) nano powder in 3, 5 and 7% wt, ciprofloxacin antibiotics, and vitamin D3 as the major materials along with electrospinning method are selected for remaking the scaffold. The fibers are smooth and beads-free and have a good diameter (around 700 nm) and porosity (above 80%) indicates ideal conditions for the treatment of damaged tissue. The membrane with 5% ceramic has been selected as ideal membrane. The mention membrane has suitable mechanical properties. Studies have shown that adding ciprofloxacin to the membrane increases the rate of hydrophilicity. The addition of β-TCP nano powder increased the bioactivity of this membrane. In terms of the drug release of ciprofloxacin and vitamin D3, 45% and 60% of drugs released within the first 24 h which in turn it shows the ability of the drugs controlled deliveration by the membrane. Accordingly, it could be concluded that this bilayer composite membrane with high bioactivity, high hydrophilicity, suitable mechanical properties, suitable morphology, and appropriate porosity has the ideal conditions for increased bone growth as well as proliferation and treatment the periodontal disease.

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“…Synthetic or natural polymers have been used in different combinations with ceramics or essential oils of medical plants to mimic the targeted tissue in various studies [6][7][8][9]. By incorporation of these materials into the polymeric scaffolds, an ideal biomaterial can be obtained via mimicking the both physical and chemical properties of the natural extracellular matrix which is the important step of the production process [10,11]. In a study, natural biosilica was isolated and incorporated with Poly (DL-lactide -co -glycolide) (PDLGA) polymer to compare the degradation pro le of pure biosilica and biosilica with PDLGA [12].…”

Section: Introductionmentioning

confidence: 99%

“…These drawbacks are accomplished in different studies by incorporating silicon dioxide (SiO 2 ) and hydroxyapatite (HAp) [11], chondroitin sulphate and gelatin [14], aleo-vera and collagen [15] into the PCL matrix. Recently, incorporation of medicinal plants into bioactive scaffolds and their controlled release in the damaged tissue is a novel approach to prevent infections, control the in ammation, as well as accelerate the skin tissue regeneration such as ulcers and burns healing [9].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Composite Nanofibers Based on Poly (ε-Caprolactone) and Styrax Liquidus (Liquidambar orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results

et al. 2022

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In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mil. incorporated PCL brous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nano bers were examined for their antioxidant activity, anti-bio lm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nano bers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic broblast cells. The brous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.

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Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering

Cited by 26 publications

References 32 publications

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

3D Printed Poly(?-Caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on Composite Preparation by Melt Blending or Solvent Casting Techniques and Influence of Bioceramic Content on Scaffold Properties

Preparation and evaluation of bioactive bilayer composite membrane PHB/β‐TCP with ciprofloxacin and vitamin D3 delivery for regenerative damaged tissue in periodontal disease

Electrospun Composite Nanofibers Based on Poly (ε-Caprolactone) and Styrax Liquidus (Liquidambar orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results

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