Development of novel electrochemical sensor for the detection of biological warfare agents: enzyme, antibody, and DNA free

Abstract

There is an urgent need to develop a novel sensor that can quickly and accurately detect biological warfare agents (BWAs). A novel electrochemical sensor was specially designed and developed for Staphylococcus aureus as a potential BWAs without using any enzyme, antibody, or genetic material. Cyclic voltammetry (CV), linear resistance polarization (LRP), chronoamperometry, impedance spectroscopy, and Differential Pulse Voltammetry (DPV) techniques were used for fast and accurate detection of BWAs. Co-precipitation technique was used for the synthesis of CuO nanoparticles (NPs) for their use in the electrochemical sensor. The effect of temperature, stirring and addition of CuO NPs was thoroughly investigated. The storage, stability, and performance of electrochemical sensor was tested for 45 days. The current response of the electrochemical sensor was found linear with the correlation coefficient, R 2 = 0.991. The major advantage of this novel electrochemical sensor is that immobilization of enzyme, antibody, or DNA is not required in sensing technology as the immobilization process makes biosensors a highly specific. The novel electrochemical sensor was very quick, accurate, non-specific, and provide reliable results in the qualitative and quantitative estimation of pathogenic bacteria as a potential BWAs.

Hetero-Single-Atom F and P-Doped Layered Graphitic Carbon Nitride Nanosheets for Electrochemical Quantification of Vanillin in Food Samples

“…In contrast, electrochemical-based techniques stand out as a more reliable approach, offering accuracy and sustainable results. To date, various electrochemical sensors have been developed using numerous materials for a variety of applications including the detection of biological molecules, biowarfare agents, , dyes, pesticides, , environmental pollutants, and chemicals, as well as for food safety applications. , Herein, we report the synthesis and construction of fluorine (F) and phosphorus (P) codoped layered graphitic carbon nitride (FP-gCN) and a FP-gCN-modified electrode. The resultant FP-gCN formation is characterized and confirmed by using various physicochemical techniques.…”

A novel sensor for antiviral drug Favipiravir in the treatment of COVID-19 designed by G/In 2 O 3 nanomaterial

“…In the literature, several analytical techniques have been stated for the detection of FVP in pharmaceutical and biological samples, in addition to high-performance liquid chromatography (HPLC) and ultraviolet spectra (UV) [15], colorimetric analysis method (CAM) [16], thin layer chromatography (TLC) [17], and liquid chromatography-tandem mass spectrometry (LC-MS) [18]. Among the most extensively used instrumental methods, the electrochemical technique offers a suitable method with good physicochemical properties such as being inexpensive, having high catalytic activity, high sensitivity, and selectivity [19] [20].Thus, electrochemical sensors are broadly used to determine trace amounts of drugs and other analytes in pharmaceutical, biological, and environmental samples [21]. Electrochemical sensors have to be enhanced with good materials to improve their analytical performance.…”