Development of novel Meju starter culture using plant extracts with reduced Bacillus cereus counts and enhanced functional properties

Shukla̽, Shruti; Park, Juyeon; Park, Jung Hyun; Lee, Jong Suk; Kim, Myung‐Hee

doi:10.1038/s41598-017-09551-0

Cited by 14 publications

(11 citation statements)

References 59 publications

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“…The moderately acidic pH in the Doenjang samples attests it safe for consume, contrary to the excessively low pH which adversely affects health by causing acidosis. The results are consistent with previous findings, signifying the role of starter culture and alterations in processing conditions and ingredients on the pH of prepared Doenjang 6,15 .…”

Section: Resultssupporting

confidence: 92%

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Garlic augments the functional and nutritional behavior of Doenjang, a traditional Korean fermented soybean paste

Bahuguna

Shukla̽

Lee

et al. 2019

Sci Rep

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Three different forms of garlic, namely, fresh garlic (2%, 6%, 10%), heat-dried (1%, 2%, 3%) and freeze-dried (1%, 2%, 3%), were supplemented in soybean paste to prepare Doenjang and further evaluated for functional, nutritional and safety aspects. Results showed a considerable antioxidant and anti-proliferative activity of garlic-supplemented Doenjang . As a measure of nutritive value, a high amount of total free amino acids, 4,290.73 mg/100 g–5,492.94 mg/100 g, was observed in prepared Doenjang . Among all preparations, 3% freeze-dried garlic-supplemented Doenjang proved the most effective against gastric adenocarcinoma and lung adenocarcinoma with 50% inhibition concentration of 7.66 ± 0.53 mg/mL and 7.82 ± 0.34 mg/mL, respectively. However 10% fresh-garlicsupplemented Doenjang (GGD-10) showed better activity against colorectal adenocarcinoma (HT29) cell line. Furthermore, GGD-10 effectively reduced colony formation and altered mitochondrial membrane potential of HT29 cells. Absence of pathogenic bacteria ( Staphylococcus aureus, Salmonella species and Bacillus cereus ) and aflatoxin was observed in Doenjang samples. In addition, nontoxic amount of anti-nutritional biogenic amines was observed in all the samples. The results collectively suggest that the addition of garlic in Doenjang can improve its nutritional and functional value, resulting in the protection of consumers from protein deficiencies and various stress conditions.

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Section: Resultssupporting

confidence: 92%

“…Results are assisted with the number of reports presenting the diverse amount of phenolics and flavonoids in the soy fermented food 24,25 . In a recent study, variations in phenolic and flavonoid contents were reported in Doenjang formed by the addition of different plant materials 15 .…”

Section: Resultsmentioning

confidence: 99%

Garlic augments the functional and nutritional behavior of Doenjang, a traditional Korean fermented soybean paste

Bahuguna

Shukla̽

Lee

et al. 2019

Sci Rep

Self Cite

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“…Although the anti-bacterial, anti-oxidant, and anti-obesity effects of lotus and its extracts have been known for many years [24,52], little is known about the activity of LAE on cancer cell migration. We demonstrated that LAE had the potential inhibitory activity for cell migration and EMT in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Lotus Leaf Extract Inhibits the Cell Migration and Metastasis of ER- Breast Cancer

Tong¹,

Li²,

Wu³

et al. 2020

Preprint

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Background: Patients with estrogen receptor negative (ER-) breast cancer have poor prognosis because of their high rates of metastasis. However, there is no effective treatment and drugs for ER− breast cancer metastasis. Our purpose of this study was to evaluate the effect and mechanism of lotus leaf alcohol extract (LAE) on the cell migration and metastasis of ER- breast cancer.Methods: The anti-migratory effect and mechanism of LAE were analysed in ER- breast cancer cells including SK-BR-3, MDA-MB-231 and HCC1806. Cell viability assay, wound-healing assay, RNA-sequence analysis and immunoblotting assay were applied in examining the cytotoxicity, anti-migratory effect and its possible pathways of LAE. To further investigate the inhibitory effect of LAE on metastasis in vivo, subcutaneous xenograft nude mice model and intravenous injection nude mice model were established. Lung and liver tissues were analysed by the Hematoxylin & eosin staining and immunoblotting assay.Results: We found that lotus leaf alcohol extract (LAE), not nuciferine, inhibited cell migration significantly in SK-BR-3, MDA-MB-231 and HCC1806 breast cancer cells, and did not change viability of breast cancer cells. The anti-migratory effect of LAE was dependent on TGF-β1 signaling, while independent of Wnt signaling and autophagy influx. Intracellular H2O2 participated in the TGF-β1-related inhibition of cell migration. LAE inhibited significantly the breast cancer cells metastasis in mice models. RNA-sequence analysis showed that extracellular matrix signaling pathways are associated with LAE-suppressed cell migration.Conclusions: Our findings demonstrated that lotus leaf alcohol extract inhibits the cell migration and metastasis of ER- breast cancer via TGF-β1/Erk1/2 and TGF-β1/SMAD3 signaling, which provides a potential therapeutic strategy for ER- breast cancer.

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“…All the collected ltrate was concentrated to dryness by evaporation on a rotary evaporator, and then dissolved in DMSO and ltered by a membrane lter with 0.22μm pore size (Millipore, USA). Finally, LAE was diluted into several concentrations (10,25,50, 100, 250µg/ml) for cell treatments and stored at 4 o C.…”

Section: Lae Treamentmentioning

confidence: 99%

Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis

Tong

et al. 2020

Preprint

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Background: Patients with estrogen receptor negative (ER-) breast cancer have poor prognosis due to high rates of metastasis. However, there is no effective treatment and drugs for ER- breast cancer metastasis. Our purpose of this study was to evaluate the effect of lotus leaf alcohol extract (LAE) on the cell migration and metastasis of ER- breast cancer.Methods: The anti-migratory effect of LAE were analyzed in ER- breast cancer cells including SK-BR-3, MDA-MB-231 and HCC1806 cell lines. Cell viability assay, wound-healing assay, RNA-sequence analysis and immunoblotting assay were used to evaluate the cytotoxicity and anti-migratory effect of LAE. To further investigate the inhibitory effect of LAE on metastasis in vivo, subcutaneous xenograft and intravenous injection nude mice models were established. Lung and liver tissues were analyzed by the Hematoxylin & eosin staining and immunoblotting assay. Results: We found that lotus leaf alcohol extract (LAE), not nuciferine, inhibited cell migration significantly in SK-BR-3, MDA-MB-231 and HCC1806 breast cancer cells, and did not affect viability of breast cancer cells. The anti-migratory effect of LAE was dependent on TGF-β1 signaling, while independent of Wnt signaling and autophagy influx. Intracellular H2O2 was involved in the TGF-β1-related inhibition of cell migration. LAE inhibited significantly the breast cancer cells metastasis in mice models. RNA-sequence analysis showed that extracellular matrix signaling pathways are associated with LAE-suppressed cell migration. Conclusions: Our findings demonstrated that lotus leaf alcohol extract inhibits the cell migration and metastasis of ER- breast cancer, at least in part, via TGF-β1/Erk1/2 and TGF-β1/SMAD3 signaling pathways, which provides a potential therapeutic strategy for ER- breast cancer.

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Development of novel Meju starter culture using plant extracts with reduced Bacillus cereus counts and enhanced functional properties

Cited by 14 publications

References 59 publications

Garlic augments the functional and nutritional behavior of Doenjang, a traditional Korean fermented soybean paste

Garlic augments the functional and nutritional behavior of Doenjang, a traditional Korean fermented soybean paste

Lotus Leaf Extract Inhibits the Cell Migration and Metastasis of ER- Breast Cancer

Lotus leaf extract inhibits ER- breast cancer cell migration and metastasis

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