Development of Novel Paclitaxel-Loaded ZIF-8 Metal-Organic Framework Nanoparticles Modified with Peptide Dimers and an Evaluation of Its Inhibitory Effect against Prostate Cancer Cells

Zhao, Heming; Gong, Liming; Wu, Hao; Liu, Chao; Liu, Yanhong; Xiao, Cheng; Liu, Chun‐Jen; Chen, Liqing; Jin, Mingji; Gao, Zhonggao; Guan, Youyan; Huang, Wei

doi:10.3390/pharmaceutics15071874

Cited by 11 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is because the lipid monolayer coating of nanoparticles increased the cell membrane interaction with time . Zhao et al reported courmarin-6-loaded Di-PEG-ZIF nanoparticles showed 1.8-fold cellular uptake in Lncap cells in 2 h of study . For our proposed system, it could be possible that nanocarriers were internalized through the endocytosis process, in which the nanoparticles initially stayed in the cytoplasm before lysozyme is released and traveled to the nuclear or perinuclear region .…”

Section: Resultsmentioning

confidence: 93%

“…107 Zhao et al reported courmarin-6loaded Di-PEG-ZIF nanoparticles showed 1.8-fold cellular uptake in Lncap cells in 2 h of study. 108 For our proposed system, it could be possible that nanocarriers were internalized through the endocytosis process, in which the nanoparticles initially stayed in the cytoplasm before lysozyme is released and traveled to the nuclear or perinuclear region. 109 we also observed colocalization of intracellular fluorescence after 24 h, which could indicate undissociated protein and polymer particles.…”

Section: Cytotoxicity Study Of Protein-encapsulated Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Khan,

Armstrong,

Lenertz

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Developing protein confinement platforms is an attractive research area that not only promotes protein delivery but also can result in artificial environment mimicking of the cellular one, impacting both the controlled release of proteins and the fundamental protein biophysics. Polymeric nanoparticles (PNPs) are attractive platforms to confine proteins due to their superior biocompatibility, low cytotoxicity, and controllable release under external stimuli. However, loading proteins into PNPs can be challenging due to the potential protein structural perturbation upon contacting the interior of PNPs. In this work, we developed a novel approach to encapsulate proteins in PNPs with the assistance of the zeolitic imidazolate framework (ZIF). Here, ZIF offers an additional protection layer to the target protein by forming the protein@ZIF composite via aqueous-phase cocrystallization. We demonstrated our platform using a model protein, lysozyme, and a widely studied PNP composed of poly(ethylene glycol)poly(lactic-co-glycolic acid) (PEG−PLGA). A comprehensive study via standard loading and release tests as well as various spectroscopic techniques was carried out on lysozyme loaded onto PEG−PLGA with and without ZIF protection. As compared with the direct protein encapsulation, an additional layer with ZIF prior to loading offered enhanced loading capacity, reduced leaching, especially in the initial stage, led to slower release kinetics, and reduced secondary structural perturbation. Meanwhile, the function, cytotoxicity, and cellular uptake of proteins encapsulated within the ZIF-bound systems are decent. Our results demonstrated the use of ZIF in assisting in protein encapsulation in PNPs and established the basis for developing more sophisticated protein encapsulation platforms using a combination of materials of diverse molecular architectures and disciplines. As such, we anticipate that the protein-encapsulated ZIF systems will serve as future polymer protein confinement and delivery platforms for both fundamental biophysics and biochemistry research and biomedical applications where protein delivery is needed to support therapeutics and/or nutrients.

show abstract

Section: Resultsmentioning

confidence: 93%

Section: Cytotoxicity Study Of Protein-encapsulated Nanoparticlesmentioning

confidence: 99%

Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Khan,

Armstrong,

Lenertz

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Zhao et al have developed a cancer-targeting system using MOF and di-peptides [63]. MOF, a nanocarrier, is a porous material synthesized using a coordination bond between a metal ion and an organic linker and has the advantages of adjustable porosity and structure, high surface area, and the ability to load various drugs [64][65][66][67].…”

Section: Receptor-mediated Peptidementioning

confidence: 99%

Role of Functionalized Peptides in Nanomedicine for Effective Cancer Therapy

Kim,

Park

2024

Biomedicines

View full text Add to dashboard Cite

Peptide-functionalized nanomedicine, which addresses the challenges of specificity and efficacy in drug delivery, is emerging as a pivotal approach for cancer therapy. Globally, cancer remains a leading cause of mortality, and conventional treatments, such as chemotherapy, often lack precision and cause adverse effects. The integration of peptides into nanomedicine offers a promising solution for enhancing the targeting and delivery of therapeutic agents. This review focuses on the three primary applications of peptides: cancer cell-targeting ligands, building blocks for self-assembling nanostructures, and elements of stimuli-responsive systems. Nanoparticles modified with peptides improved targeting of cancer cells, minimized damage to healthy tissues, and optimized drug delivery. The versatility of self-assembled peptide structures makes them an innovative vehicle for drug delivery by leveraging their biocompatibility and diverse nanoarchitectures. In particular, the mechanism of cell death induced by self-assembled structures offers a novel approach to cancer therapy. In addition, peptides in stimuli-responsive systems enable precise drug release in response to specific conditions in the tumor microenvironment. The use of peptides in nanomedicine not only augments the efficacy and safety of cancer treatments but also suggests new research directions. In this review, we introduce systems and functionalization methods using peptides or peptide-modified nanoparticles to overcome challenges in the treatment of specific cancers, including breast cancer, lung cancer, colon cancer, prostate cancer, pancreatic cancer, liver cancer, skin cancer, glioma, osteosarcoma, and cervical cancer.

show abstract

“…Talking about imidazole acceptability in other chemical biology-oriented approaches, it has shown tremendous potential. These include serving as pH-responsive potent antimicrobials [ 164 ], improving the photophysical characteristics of azo dyes [ 165 , 166 , 167 ], decreasing side toxicities [ 13 , 168 , 169 , 170 ], and successful chemical integration in polymeric materials as theragnostic applications (such as in zeolitic imidazolate framework applications in drug delivery) [ 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 ].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Imidazoles as Serotonin Receptor Modulators for Treatment of Depression: Structural Insights and Structure–Activity Relationship Studies

Goel,

Thapliyal,

Kharb

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

Serotoninergic signaling is identified as a crucial player in psychiatric disorders (notably depression), presenting it as a significant therapeutic target for treating such conditions. Inhibitors of serotoninergic signaling (especially selective serotonin reuptake inhibitors (SSRI) or serotonin and norepinephrine reuptake inhibitors (SNRI)) are prominently selected as first-line therapy for the treatment of depression, which benefits via increasing low serotonin levels and norepinephrine by blocking serotonin/norepinephrine reuptake and thereby increasing activity. While developing newer heterocyclic scaffolds to target/modulate the serotonergic systems, imidazole-bearing pharmacophores have emerged. The imidazole-derived pharmacophore already demonstrated unique structural characteristics and an electron-rich environment, ultimately resulting in a diverse range of bioactivities. Therefore, the current manuscript discloses such a specific modification and structural activity relationship (SAR) of attempted derivatization in terms of the serotonergic efficacy of the resultant inhibitor. We also featured a landscape of imidazole-based development, focusing on SAR studies against the serotoninergic system to target depression. This study covers the recent advancements in synthetic methodologies for imidazole derivatives and the development of new molecules having antidepressant activity via modulating serotonergic systems, along with their SAR studies. The focus of the study is to provide structural insights into imidazole-based derivatives as serotonergic system modulators for the treatment of depression.

show abstract

Development of Novel Paclitaxel-Loaded ZIF-8 Metal-Organic Framework Nanoparticles Modified with Peptide Dimers and an Evaluation of Its Inhibitory Effect against Prostate Cancer Cells

Cited by 11 publications

References 31 publications

Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Metal–Organic Framework Induced Stabilization of Proteins in Polymeric Nanoparticles

Role of Functionalized Peptides in Nanomedicine for Effective Cancer Therapy

Imidazoles as Serotonin Receptor Modulators for Treatment of Depression: Structural Insights and Structure–Activity Relationship Studies

Contact Info

Product

Resources

About