Development of Novel Protease Assay Device Using a Nanoimprinted Two-dimensional Photonic Crystal

Hashimoto, Wakana; Endo, Tatsuro; Sueyoshi, Kenji; Hisamoto, Hideaki

doi:10.1246/cl.140674

Cited by 14 publications

(15 citation statements)

References 13 publications

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“…. Based on our previous reports using 2D‐PhC‐based optical biosensors , the reflection peak, which depends on the periodicity and size, can be observed based on Bragg's law. Furthermore, to perform the label‐free detection of antigen‐antibody reaction on the 2D‐PhC surfaces, surrounding refractive index (RI) change on the 2D‐PhC surface by an antibody‐antigen reaction can be monitored as a reflection peak intensity change based on the Fresnel reflection.…”

Section: Methodsmentioning

confidence: 98%

Label‐free optical detection of C‐reactive protein by nanoimprint lithography‐based 2D‐photonic crystal film

Endo

Kajita²,

Kawaguchi

et al. 2016

Biotechnology Journal

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The development of high-sensitive, and cost-effective novel biosensors have been strongly desired for future medical diagnostics. To develop novel biosensor, the authors focused on the specific optical characteristics of photonic crystal. In this study, a label-free optical biosensor, polymer-based two-dimensional photonic crystal (2D-PhC) film fabricated using nanoimprint lithography (NIL), was developed for detection of C-reactive protein (CRP) in human serum. The nano-hole array constructed NIL-based 2D-PhC (hole diameter: 230 nm, distance: 230, depth: 200 nm) was fabricated on a cyclo-olefin polymer (COP) film (100 µm) using thermal NIL and required surface modifications to reduce nonspecific adsorption of target proteins. Antigen-antibody reactions on the NIL-based 2D-PhC caused changes to the surrounding refractive index, which was monitored as reflection spectrum changes in the visible region. By using surface modified 2D-PhC, the calculated detection limit for CRP was 12.24 pg/mL at an extremely short reaction time (5 min) without the need for additional labeling procedures and secondary antibody. Furthermore, using the dual-functional random copolymer, CRP could be detected in a pooled blood serum diluted 100× with dramatic reduction of nonspecific adsorption. From these results, the NIL-based 2D-PhC film has great potential for development of an on-site, high-sensitivity, cost-effective, label-free biosensor for medical diagnostics applications.

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Section: Methodsmentioning

confidence: 98%

Label‐free optical detection of C‐reactive protein by nanoimprint lithography‐based 2D‐photonic crystal film

Endo

Kajita²,

Kawaguchi

et al. 2016

Biotechnology Journal

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“…In our previous study, we detected various target molecules, by using 2D-PhCs without a cavity that were modified with functional molecules such as antibodies, substrates, and ionophores. [1][2][3][4] Therefore, owing to the polymer-based 2D-PhC cavity and functional molecules, more sensitive and selective detection of target molecules using visible light is expected in the near future.…”

Section: Discussionmentioning

confidence: 99%

“…Two-dimensional photonic crystals (2D-PhCs) have attracted considerable attention in various fields, including optical sensors, [1][2][3][4][5][6] lasers, [7][8][9][10][11] and optical communication systems. [12][13][14] The main optical characteristic of 2D-PhCs is the light reflection of a specific wavelength region, called the photonic bandgap (PBG), which is based on the Bragg diffraction, determined by the wavelength-dimension periodic structure of the PhC.…”

Section: Introductionmentioning

confidence: 99%

Polymer-based Photonic Crystal Cavity Sensor for Optical Detection in the Visible Wavelength Region

et al. 2016

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In this study, a polymer-based two-dimensional photonic crystal (PhC) cavity for visible-light-based optical-sensing applications was designed and fabricated for the first time. The PhC cavity configuration was designed to operate at 650 nm, and fabricated with a polymer (resist) on a silicon substrate using electron-beam lithography. For investigating sensing applications based on shifting of condition exhibiting a photonic bandgap (PBG), the polymer monolayer deposition (layer-by-layer method) was monitored as the light-intensity change at the cavity position. Consequently, the monolayer-level detection of polyions was achieved.

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“…After the O 2 plasma treatment, anti-human fibrinogen antibody was immobilized (1 μg/ml, 1h at room temperature) via a surface modification using γ-APTES (0.1% (v/v)) (1 h, room temperature) and GA (0.5% (v/v)) (1 h, room temperature). And then, after the immobilization of antibody, ethanolamine solution (100 mM) (1 h, room temperature) was introduced for blocking [16]. From these anti-body immobilization procedure, detection of fibrinogen was carried out.…”

Section: Antibody Immobilizationmentioning

confidence: 99%

Label-Free Optical Detection of Fibrinogen in Visible Region Using Nanoimprint Lithography-Based Two-Dimensional Photonic Crystal

Endo

Kajita²

2017

IEICE Trans. Electron.

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SUMMARYFor the future medical diagnostics, high-sensitive, rapid, and cost effective biosensors to detect the biomarkers have been desired. In this study, the polymer-based two-dimensional photonic crystal (2D-PC) was fabricated using nanoimprint lithography (NIL) for biosensing application. In addition, for biosensing application, label-free detection of fibrinogen which is a biomarker to diagnose the chronic obstructive pulmonary disease (COPD) could be achieved using antigen-antibody reaction high-sensitively (detection limit: pg/ml order) and rapidly. Using this polymer-based 2D-PC, optical biosensor can be developed cost effectively. Furthermore, by using polymer as a base material for fabrication of 2D-PC, label-free detection of antigen-antibody reaction can be performed in visible region.

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Development of Novel Protease Assay Device Using a Nanoimprinted Two-dimensional Photonic Crystal

Cited by 14 publications

References 13 publications

Label‐free optical detection of C‐reactive protein by nanoimprint lithography‐based 2D‐photonic crystal film

Label‐free optical detection of C‐reactive protein by nanoimprint lithography‐based 2D‐photonic crystal film

Polymer-based Photonic Crystal Cavity Sensor for Optical Detection in the Visible Wavelength Region

Label-Free Optical Detection of Fibrinogen in Visible Region Using Nanoimprint Lithography-Based Two-Dimensional Photonic Crystal

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