Label-Free Optical Detection of Fibrinogen in Visible Region Using Nanoimprint Lithography-Based Two-Dimensional Photonic Crystal

Abstract

SUMMARYFor the future medical diagnostics, high-sensitive, rapid, and cost effective biosensors to detect the biomarkers have been desired. In this study, the polymer-based two-dimensional photonic crystal (2D-PC) was fabricated using nanoimprint lithography (NIL) for biosensing application. In addition, for biosensing application, label-free detection of fibrinogen which is a biomarker to diagnose the chronic obstructive pulmonary disease (COPD) could be achieved using antigen-antibody reaction high-sensitively (detection limit: pg/ml order) and rapidly. Using this polymer-based 2D-PC, optical biosensor can be developed cost effectively. Furthermore, by using polymer as a base material for fabrication of 2D-PC, label-free detection of antigen-antibody reaction can be performed in visible region.

Template Stripping Method-Based Au Nanoarray for Surface-Enhanced Raman Scattering Detection of Antiepileptic Drug

“…The polymer-based template—fabricated using NIL—was used to fabricate the PC. Using this design of polymer-based template, we developed a nanophotonic-based biosensor based on a photonic crystal (PhC) [ 26 , 27 , 28 , 29 , 30 , 31 ]. In addition, using this polymer-based template mold, the metallic layer deposited can be peeled off easily.…”

“…By using a polymer-based template for fabrication of SERS active substrate, SERS active substrate can be fabricated with high reproducibility by changing the polymer-based template. We have fabricated polymer-based nanostructures using NIL to develop nanophotonics-based biosensors that operate in the visible region [ 26 , 27 , 28 , 29 , 30 , 31 ]. The surface energy of a polymer is generally low.…”