Development of novel short-term heating angioplasty: diameter and elasticity change of vascular wall ex vivo

Shimazaki, Natsumi; Kaneko, Kenji; Nakatani, Eriko; Arai, Tsunenori

doi:10.1117/12.701797

Cited by 3 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have obtained sufficient arterial dilatation with short-duration heating and low dilatation pressure on both acute and chronic phase in our previous in vivo canine and rabbit studies. 2 , 14 , 24 The mechanism of our short-duration heating dilatation have been reported 21 ; (i) reversible collagen thermal denaturation and stretch-fixing of elastin occur during short-duration heating balloon dilatation, and (ii) collagen fiber orients circumferentially and reconstructs with the temperature decrease after short-duration heating. Therefore, the arterial dilatation with our short-duration heating dilatation was performed without any vascular injuries, which are the main causes of the restenosis after the balloon angioplasty.…”

Section: Introductionmentioning

confidence: 88%

“…We have proposed a novel short-duration thermal angioplasty with uniform temperature distribution, Photo-thermo Dynamic Balloon Angioplasty 20 , 21 to reduce thermal damages to the adventitia and surrounding tissue, which were the major cause of restenosis after other thermal angioplasties. Short-duration heating (<15 s, <70 °C) is realized by the combination of laser light irradiation and continuous constant fluid flow inside the balloon.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Deformation Rate of Smooth Muscle Cells in Vessel Walls After Short-Duration Heating Dilatation in a Porcine Model Ex Vivo and In Vivo

Kunio

Arai

2012

Cardiovasc Eng Tech

Self Cite

View full text Add to dashboard Cite

We have proposed a novel short-duration thermal angioplasty with uniform temperature distribution. Although the dilatation mechanism of our short-duration heating dilatation was reported in our previous study, the influences on smooth muscle cells (SMCs) are not sufficiently understood. We studied the influences on SMCs in terms of shape change and discussed the relationship between the SMCs’ shape change and dilatation mechanism ex vivo and in vivo. We found that the SMCs were fixed in the stretched condition after our short-duration heating dilatation both ex vivo and in vivo. The deformation rate of SMCs’ shape, measured by the cells’ nuclei, was increased with rising balloon maximum temperature (Tballoon), and the same tendency was observed for the arterial dilatation rate. We hypothesize that the SMCs were fixed in the stretched condition because the arterial dilatation with our short-duration heating dilatation was performed without any plastic deformations of the vessel wall, causing the vessel wall itself to be stretched. We also prospect that the reasons for the positive correlation between the deformation rate of SMCs’ shape and Tballoon are that (i) the area heated over 60 °C was expanded with rising Tballoon, and (ii) the arterial dilatation rate was also increased with rising Tballoon.

show abstract

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

The Deformation Rate of Smooth Muscle Cells in Vessel Walls After Short-Duration Heating Dilatation in a Porcine Model Ex Vivo and In Vivo

Kunio

Arai

2012

Cardiovasc Eng Tech

Self Cite

View full text Add to dashboard Cite

show abstract

“…The carotid arteries of domestic pigs (6 months of age; weighing 110-120 kg) removed within 12 h of culling were used. A CAW sample (length, approximately 2 cm) was immersed in a heated (50-70 C) uorophore solution for 15 s to observe delivery of the agent to the CAW sample under the condition of reversible thermal denaturation [11,14,15]. The concentration of rhodamine B was 3.5 µg/cm 3 , and that of OGLP was 1.0 µg/cm 3 , to maintain the signal-to-noise ratio.…”

Section: Delivery Of Agents To Caw Samples Upon Heatingmentioning

confidence: 99%

“…To obtain vascular patency without heat injury, we have developed a photo-thermo dynamic balloon (PTDB) that can dilate an artery wall by softening collagen [10]. Adventitia injury is prevented as a result of brief (< 15 s, 70 C) heating using a combination of laser light irradiation and continuous uid ow within the PTDB [11]. Under this heating condition, thermal denaturation of collagen brils below the surface of the artery wall is expected, since the temperature of the surface increases to approximately 65 C [12,13].…”

Section: Introductionmentioning

confidence: 99%

Heat Enhances <i>Ex Vivo</i> Paclitaxel Delivery to Porcine Carotid Artery Wall

Homma

Shinozuka

Shimazaki³

et al. 2019

ABE

Self Cite

View full text Add to dashboard Cite

We investigated the delivery of rhodamine B and Oregon Green ®-labeled paclitaxel (OGLP) in ex vivo porcine carotid artery wall (CAW) samples after heating the reagents to 50-70 C for 15 s. When the isolated CAW samples were placed in the heated uorophore solutions, the penetration depth of the hydrophobic rhodamine B increased signi cantly compared with reference solution at 37 C. The penetration depth of OGLP also tended to increase upon heating to 70 C for 15 s. We also studied the mechanism of this agent delivery enhancement by observing the inner surface structure and hydrophobicity of the CAW samples after heating. An expanded mesh structure at the inner surface of the heated CAW samples was observed upon heating above 70 C, and the mean hydrophobicity of the media layer also increased signi cantly. We hypothesize that heating at 60-70 C for 15 s enhances the delivery of uorophores to CAW samples as a result of an expanded mesh structure at the inner surface of the CAW, along with a simultaneous increase in hydrophobicity.

show abstract

“…We had reported the feasibility of the PTDB angioplasty by in vivo animal experiments [6 8]. We had also investigated on the PTDB dilatation mechanism by ex vivo basic study [9,10]. In these reports, we suggested that the principle of the PTDB angioplasty should be suspension of the dilated artery shape by collagen restructuring with thermal denaturation.…”

Section: Introductionmentioning

confidence: 97%

Development of novel short-term heating angioplasty: thermal denaturation dynamics of collagen in artery wall

et al. 2009

View full text Add to dashboard Cite

We have studied to develop the new thermal angioplasty methodology, photo-thermo dynamic balloon angioplasty (PTDBA), which provides artery dilatation with short-term (<15s) and uniform heating through the balloon by the combination of the efficient laser driven heat generation and fluid perfusion. Thermal denaturation degree of the collagen in artery media may be the important factor to attain sufficient artery dilatation for the PTDBA. In order to predict the optimum heating condition i.e. the balloon temperature and heating duration, we investigated the thermal denaturation dynamics of artery collagen in ex vivo. The extracted fresh porcine carotid artery was used. The temperature-dependent light scattering property and mechanical property of the artery specimen were simultaneously measured during artery temperature rising by specially made setup to assess the denaturation of arterial collagen. The change rate of the backscattered light intensity from the artery specimen; I(T)/I 0 with 633nm was measured to evaluate the artery scattering property change with the thermal denaturation. The artery specimen was heated from 25°C to 80°C with constant temperature rising rate of 3°C/min. The measured I(T)/I 0 was suddenly increased over 48°C. This boundary temperature might be the initiation temperature of the arterial collagen denaturation. We defined the variation of the I(T)/I 0 as the collagen denaturation ratio, and calculated the reactive enthalpy by the chemical equilibrium theory. Since the calculated enthalpy was similar to the enthalpy in literature report, the variety of I(T)/I 0 during the temperature rising might be attributed to the collagen conformational change due to the denaturation. In terms of the artery internal force measurement, the artery force was decreased with increasing of the artery temperature up to 65°C (i.e. softening), and increased over 65°C (i.e. shrinkage). We confirmed that the changes of the backscattered light (at 633nm in wavelength) from the artery might represent the artery collagen thermal denaturation degree.

show abstract

Development of novel short-term heating angioplasty: diameter and elasticity change of vascular wall ex vivo

Cited by 3 publications

References 0 publications

The Deformation Rate of Smooth Muscle Cells in Vessel Walls After Short-Duration Heating Dilatation in a Porcine Model Ex Vivo and In Vivo

The Deformation Rate of Smooth Muscle Cells in Vessel Walls After Short-Duration Heating Dilatation in a Porcine Model Ex Vivo and In Vivo

Heat Enhances <i>Ex Vivo</i> Paclitaxel Delivery to Porcine Carotid Artery Wall

Development of novel short-term heating angioplasty: thermal denaturation dynamics of collagen in artery wall

Contact Info

Product

Resources

About