Development of novel SSR markers for evaluation of genetic diversity and population structure in Tribulus terrestris L. (Zygophyllaceae)

Abstract

Tribulus terrestris L., commonly called puncture vine and gokhru, is an important member of Zygophyllaceae. The species is highly important in context to therapeutic uses and provides important active principles responsible for treatment of various diseases and also used as tonic. It is widely distributed in tropical regions of India and the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In present study, genetic diversity and structure of different populations of T. terrestris from north India was examined at molecular level using newly developed Simple Sequence Repeat (SSR) markers. In total, 20 primers produced 48 alleles in a size range of 100–500 bp with maximum (4) fragments amplified by TTMS-1, TTMS-25 and TTMS-33. Mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.368 and 1.01, respectively. Dendrogram showed three groups, one of which was purely containing accessions from Rajasthan while other two groups corresponded to Punjab and Haryana regions with intermixing of few other accessions. Analysis of molecular variance partitioned 76 % genetic variance within populations and 24 % among populations. Bayesian model based STRUCTURE analysis detected two genetic stocks for analyzed germplasm and also detected some admixed individuals. Different geographical populations of this species showed high level of genetic diversity. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0469-8) contains supplementary material, which is available to authorized users.

Climate Change and Fading Genetic Resources of <em>Parkia biglobosa</em> (Jacq.) in Nigeria Based on SSR Markers

“…The mean PIC which represent allele diversity was moderate (0.35) and similar to the report of Kaur et al [35] for Tribulus terrestris but lower to the values reported by Popoola et al [36]. Similarly, the mean percent polymorphism (74.16%) was high and comparable to previous reports on forest trees including P. Biglobosa [35][36][37]. The SSR markers amplified at least two or more than three fragments and recorded a PIC value of 0.35 which is an indicator of high polymorphism.…”

“…The range of alleles (9 -14 alleles) among the markers are comparable to the reports of Lassen et al [26] and higher than previous values on some tree species [33,34]. The mean PIC which represent allele diversity was moderate (0.35) and similar to the report of Kaur et al [35] for Tribulus terrestris but lower to the values reported by Popoola et al [36]. Similarly, the mean percent polymorphism (74.16%) was high and comparable to previous reports on forest trees including P. Biglobosa [35][36][37].…”

Genetic diversity and population structure among 133 elite genotypes of sugarcane (Saccharum spp.) for use as parents in sugarcane varietal improvement

“…The final population structure was calculated with ΔK, based on the second-order rate of change of likelihood distribution mean L″(K) and with respect to K estimated using STRU CTU RE Harvester, it showed a clear peak at optimal K value (Evanno et al 2005;Earl and von-Holdt 2012). Analysis of molecular variance (AMOVA) was conducted to detect the genetic variance within and among subpopulation using GenAlEx Ver 6.5 (Peakall and Smouse 2012;Kaur et al 2016).…”

Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (Procambarus clarkii) from Lakes and Rice Fields by SSR Markers

“…Genetic diversity contributes to a species' ability to cope with environmental change and is often assessed by molecular markers. A variety of molecular markers are used to detect population genetic diversity and differentiation, including restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), and simple sequence repetition (SSR) [39][40][41]. Microsatellites are widely distributed in the genome and have high levels of polymorphism, codominance and repeatability.…”