Development of optimal conditions for lymphokine production by chicken lymphocytes

Weiler, H.; Bülow, Vicco von

doi:10.1016/0165-2427(87)90094-8

Cited by 19 publications

(6 citation statements)

References 32 publications

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“…The Con Astimulated thymocytes from young chickens were able to elaborate this chemotactic lymphokine, whereas bursal (B) cells did not produce the factor. A similar restriction of A portion of a Ph.D. dissertation in Animal Physiology, Mississippi State University, Mississippi State, MS 39762. bursacytes to produce avian lymphokines was reported by Weiler and von Bulow (1987). Thymocytes, but not bursacytes, from chickens 8 to 12 wk old cultured in the presence of Con A for 72 h produced a macrophage-activating factor and interferon activity.…”

Section: Introductionmentioning

confidence: 80%

Lymphocyte Inhibitory and Chemotactic Factors Produced by Bursal and Thymic Lymphocytes

Joshi

Glick

1990

Poultry Science

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Bursal (B) and thymic (T) lymphocytes from chickens sensitized to Mycobacterium tuberculosis (PPD) or human gamma globulin (hGG) produced an avian lymphocyte-inhibitory factor designated as LyIF-PPD or LyIF-hGG, respectively. A chemotactic factor (LCF) for peripheral blood leukocytes was elaborated only by T-cells sensitized to PPD and hGG. These factors were partially purified by HPLC and were characterized physiochemically. Maximum inhibitory activity for LyIF-PPD and LyIF-hGG occurred in peak fractions corresponding to molecular weight ranges of 29,000 to 52,000 daltons and 15,000 to 29,000 daltons, respectively. The inhibitory activity of B- and T-LyIF-hGG was lost after chymotrypsin and neuraminidase treatment. Maximum chemotactic activity for LCF-PPD and LCF-hGG was in peak HPLC fractions corresponding to molecular weight ranges of 9,000 to 16,000 daltons and 8,000 to 16,500 daltons, respectively. Chemotactic activity of LCF-PPD and LCF-hGG was lost following chymotrypsin treatment while it was not reduced after neuraminidase treatment. Both inhibitory and chemotactic activities were stable at 56 C for 30 min and resistant to changes in pH from 5 to 9. The precursor molecule for the lymphokine is made after antigen immunization, but activated in the presence of the sensitizing agent.

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Section: Introductionmentioning

confidence: 80%

Lymphocyte Inhibitory and Chemotactic Factors Produced by Bursal and Thymic Lymphocytes

Joshi

Glick

1990

Poultry Science

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“…There is very little information concerning the events which occur between interferon interaction with its receptor and gene activation, but presumably this occurs by a second message gene which acts on the IFNresponsive sequences in the noncoding portion of the genes leading to their transcription LELLEHOJ (Friedman and Stark, 1985). A chicken lymphokine having IFN activity has been detected in culture supernatant of lymphocytes stimulated with phytohemagglutinin or concanavalin A (Weiler and von Bulow, 1987;Fredericksen and Sharma, 1987). As in case of human IFN, molecular weight heterogeneity of chicken IFN (25 and 18 kDa) was caused by differences in glycosylation.…”

Section: Quantification Of Lymphokine Secretionmentioning

confidence: 96%

Lymphocytes Involved in Cell-Mediated Immune Responses and Methods to Assess Cell-Mediated Immunity

Lillehoj

1991

Poultry Science

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With increasing need for the development of vaccines against many poultry diseases, it is important to understand host immune mechanisms involved in protection against diseases. Because of increasing evidence suggesting a role of cell-mediated immunity in disease resistance against many poultry disease, investigation of immune systems involved in cell-mediated immunity is crucial for the development of immunological control strategies. Recent advances in hybridoma technology and cellular immunology have facilitated the understanding of the ontogeny, structure, and function of the avian immune system. Isolation and flow cytometric analysis of single lymphoid cells now enables researchers to dissect various components of the avian immune system and to investigate the role of subpopulations of lymphocytes in disease processes. In vitro assays are used to asses the host cellular response based upon antigen-specific lymphoproliferation, specific cellular effector function, and lymphokine secretion. These various assays can be used to quantify and qualify host immune responses and to better understand host immunity.

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“…Lymphokines (SE-ILK) from the splenic T lymphocytes of SE-immune chickens were prepared by incubating the cell suspensions (1 X 10 7 cells/ml) in RPMI 1640 medium (Sigma Chemical Company, St Louis, MO, USA) containing 7.5 of concanavalin A (Con A) in 75-cm 2 tissue culture flasks for 48 h at 37°C in a 5% CO2 incubator (Weiler & von Bulow, 1987). Lymphokines from controls (NILK) were similarly prepared by incubating the purified splenic T cells from non-immune birds with 7.5 //g/ml of Con A as above (simultaneous isolation and culture).…”

Section: Preparation Of Lymphokinesmentioning

confidence: 99%

Evaluation ofSalmonella enteritidis‐immune lymphokines on host resistance toSalmonella entericaser.gallinaruminfection in broiler chicks

et al. 1996

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The prophylactic treatment of neonatal broiler chicks with lymphokines derived from S. enteritidis-immurazed chickens (SE-ILK) was evaluated for its effect on the birds' resistance to an experimental infection S. enterica ser. gallinarum (SG). On the day of hatch, chicks were injected intraperitoneally with either SE-ILK, control non-immune lymphokines (NILK), or were left untreated. Thirty minutes later, all chicks were orally gavaged with either 10(4) colony forming units (CFU) or 10(6) CFU SG. The chicks were observed twice daily for 10 days for morbidity and mortality. Chicks that died during the experiment had their livers cultured for SG. The survivors were killed and their livers, spleens and caecal tonsils cultured for SG. The prophylactic treatment of chickens with SE-ILK induced significant protection against extraintestinal SG infection when compared to the NILK-treated or non-treated controls as evidenced by: (1) a significant reduction (P< 0.005) in the mortality of chicks challenged with either 10(4) and 10(6) CFU SG; (2) an increased average weight gains of chicks challenged with either 10(4) and 10(6) CFU SG; and (3) a significant (P< 0.001) reduction in the number of chicks with organs culture-positive for SG. The results suggest that the prophylactic administration of SE-ILK can confer non-specific protection to chicks against a pathogenic species of Salmonella resulting in reduced morbidity, mortality, and organ infectivity caused by SG infections of broiler chicks, while enhancing performance during the first 10 days of Ufe.

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Development of optimal conditions for lymphokine production by chicken lymphocytes

Cited by 19 publications

References 32 publications

Lymphocyte Inhibitory and Chemotactic Factors Produced by Bursal and Thymic Lymphocytes

Lymphocyte Inhibitory and Chemotactic Factors Produced by Bursal and Thymic Lymphocytes

Lymphocytes Involved in Cell-Mediated Immune Responses and Methods to Assess Cell-Mediated Immunity

Evaluation ofSalmonella enteritidis‐immune lymphokines on host resistance toSalmonella entericaser.gallinaruminfection in broiler chicks

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