Development of Oral Vaccines to Stimulate Mucosal and Systemic Immunity: Barriers and Novel Strategies

Shalaby, Waleed S.W.

doi:10.1006/clin.1995.1019

Cited by 88 publications

(56 citation statements)

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“…The oral mode of delivery has the potential to confer long-term mucosal immunity against pathogens such as HIV-1 if it can induce antigen specific cells to home to the gut as shown in previous studies [25][26][27][28], but efforts to stimulate immunity through this route may inadvertently induce oral tolerance instead [29]. Oral vaccine development using replication defective adenovirus, such as the vector we have tested, may be impeded by vector instability in the acidic environment of the stomach, through which it must pass to ensure antigen presentation and maximum exposure to and uptake by the antigen presenting cells of the intestine.…”

Section: Discussionmentioning

confidence: 99%

Intramuscular rather than oral administration of replication-defective adenoviral vaccine vector induces specific CD8+ T cell responses in the gut

Lin

Cun

Harris-McCoy

et al. 2007

Vaccine

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Gut-associated lymphoid tissue (GALT) is the primary replication site for HIV-1, resulting in a pronounced CD4 + T cell loss in this tissue during primary infection. A mucosal vaccine that generates HIV-specific CD8 + T cells in the gut could prevent the establishment of founder populations and broadcasting of virus. Here, we immunized mice orally and systemically with a chimpanzee derived adenoviral vector expressing HIV gag (AdC68gag) and measured frequencies of gag-specific interferon-gamma (IFN-γ) producing CD8 + T cells in the GALT. A single oral administration was inefficient at eliciting responses in the mesenteric lymph nodes and Peyer's Patches, while a single intramuscular administration elicited strong systemic and detectable mucosal responses. The gagspecific CD8 + T cell responses were present in both acute and memory phases following intramuscular administration.

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Section: Discussionmentioning

confidence: 99%

Intramuscular rather than oral administration of replication-defective adenoviral vaccine vector induces specific CD8+ T cell responses in the gut

Lin

Cun

Harris-McCoy

et al. 2007

Vaccine

View full text Add to dashboard Cite

show abstract

“…The mucosal immune system displays a unique ability to respond to an array of immunogens presented by the respiratory and oral routes. Oral vaccines are often more desirable than other routes because of easy administration to large populations and a reduced number of side effects (62). Since PLGA microspheres are readily absorbed by the Peyers patches, they have considerable potential as a vehicle for oral immunization.…”

Section: Mucosal Immunizationmentioning

confidence: 99%

Poly-DL-lactide-co-glycolide microspheres as a controlled release antigen delivery system

Lima

Júnior

1999

Braz J Med Biol Res

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Successful vaccine application means maximum protection with minimal number of administrations. A rational development of vaccines involves studies of the nature of the antigen as well as of the adjuvant to be used to improve the immune responses. This has provided the impetus for studies to design the degradable devices and for different approaches to antigen delivery by different routes of administration. The development of controlled release systems based on polymeric devices that permit a sustained or pulsed release of encapsulated antigens has attracted much interest. Polymeric delivery systems consist of polymers that release their content continuously in a controlled manner over a period of time. The development of a biocompatible delivery system for parenteral administration offers several advantages in terms of immunoadjuvanticity over other compounds. It was found that, in contrast to other carriers, microspheres are more stable, thus permitting administration by the oral or parenteral route. In the present study, we describe the main characteristics and potentialities of this new immunoadjuvant for oral and parenteral administration. Correspondence

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“…The M-cell-specific glycoproteins and glycolipids could be selectively targeted by microorganisms that adhere to M-cells and enter the host along this pathway. (JHistocbem Cytochem 44:1033-1042, tial clinical relevance for the prevention of infections, drug targeting, and M-cell-mediated vaccinations (Shalaby, 1995). Previous studies revealed specific carbohydrate compositions on the apical surface of intestinal M-cells in mice and rabbits, which are assumed to serve as binding sites for pathogenic microorganisms (Giannasca et al, 1994;Clark et al, 1993;Gebert and Hach, 1993;Jepson et al, 1993a).…”

Section: * Correspondence To: Abteilung Anatomie 2 Medizinische Hochmentioning

confidence: 99%

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

Gebert

1996

J Histochem Cytochem.

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The tonsil crypt epithelium contains membranous (M)-cells that transport antigens from the lumen to underlying lymphoid cells, thereby initiating specific immune responses. Mechanisms mediating the adhesion of antigens to the M-cell surface are important for effective and selective uptake of potential pathogens but are still poorly understood. Therefore, the carbohydrates present on crypt epithelial cells of the rabbit palatine tonsil were studied by lectin histochemistry. Ultrathin LR White sections were incubated with a panel of eight lectins conjugated to colloidal gold or biotin. The glycocalyx of the apical membrane of M-cells was selectively labeled by UEA-I, LTA, HPA, and VVA, whereas that of the remaining squamous epithelial cells preferentially bound RCA-I and PNA. WGA and ConA showed only little binding, with no discernible preference for any of the cell types. Double labeling of UEA-1 together with anti-vimentin antibodies revealed that UEA-I-positive epithelial cells also contained the rabbit M-cell marker vimentin, and vice versa. The results show that a specific composition of glycoconjugates, which differs from that on squamous epithelial cells, is found on M-cells of the rabbit tonsil. The M-cell-specific glycoproteins and glycolipids could be selectively targeted by microorganisms that adhere to M-cells and enter the host along this pathway.

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Development of Oral Vaccines to Stimulate Mucosal and Systemic Immunity: Barriers and Novel Strategies

Cited by 88 publications

References 0 publications

Intramuscular rather than oral administration of replication-defective adenoviral vaccine vector induces specific CD8+ T cell responses in the gut

Intramuscular rather than oral administration of replication-defective adenoviral vaccine vector induces specific CD8+ T cell responses in the gut

Poly-DL-lactide-co-glycolide microspheres as a controlled release antigen delivery system

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

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