Development of oxaliplatin encapsulated in magnetic nanocarriers of pectin as a potential targeted drug delivery for cancer therapy

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) and oxaliplatin (OHP) were in-situ encapsulated in pectin cross-linked with Ca(2+) forming 100-200 nm sized magnetically functionalized pectin nanocarriers, referred here as MP-OHP nanocarriers. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) studies revealed formation of spherical nanostructures. The magnetic measurements by vibration sample magnetometer (VSM) revealed high saturation magnetization (M s=45.65 emu/g). The superparamagnetic property of MP-OHP was confirmed from the blocking temperature (T B) determined from field cooled and zero field cooled magnetization, measured by superconducting quantum unit interference device (SQUID) magnetometry. The stability of the aqueous dispersion of MP-OHP nanocarriers was confirmed from its high zeta potential (-30.5 mV). The drug encapsulation efficiency (55.2±4.8% w/w) and the drug loading content (0.10±0.04 wt%) in MP-OHP nanocarriers were determined from corresponding platinum contents in OHP and MP-OHP batches measured by inductively coupled plasma mass spectrometry (ICPMS). These nanocarriers exhibited a sustained release of OHP in phosphate buffer solution maintained at pH 5.5 and 7.4, where the drug release profile satisfied a combination of diffusion and swelling controlled mechanism. The cytotoxicity effect of MP-OHP nanocarriers was studied on MIA-PaCa-2 (pancreas) cancer cell line, where the GI50 values were more than 5 mg/mL and it exhibited 10 folds higher cytoxicity than the equivalent concentration of free drug.

Study of Thermal Degradation of PLGA, PLGA Nanospheres and PLGA/Maghemite Superparamagnetic Nanospheres

“…Beyond T B , the ZFC magnetization curve nearly overlapped with the FC curve, indicating superparamagnetic behavior. The T B was determined to be 71 K. Notably, the blocking temperature was corroborated by the values found in the literature for superparamagnetic iron oxide nanoparticles 34,35 . Such superparamagnetic property has demonstrated to be critical to the successful application of magnetic nanospheres in areas such as T 2 -weighted magnetic resonance imaging, drug delivery and magneto-hyperthermia 36 .…”

Green synthesis of iron oxide (Fe3O4) nanoparticles using two selected brown seaweeds: Characterization and application for lead bioremediation

“…The peaks around 6.39 keV are related to the binding energies of Fe in the biosynthesized Fe 3 O 4 -NPs colloids as reported by Dutta and Sahu (2012). Therefore, the EDX spectra for the Fe 3 O 4 -NPs confirmed the presence of Fe 3 O 4 -NPs in the two biosynthesized nano colloid.…”

Superparamagnetic maghemite loaded poly (ε- caprolactone) nanocapsules : characterization and synthesis optimization

“…Beyond T B , the ZFC magnetization curve nearly overlapped with the FC curve, indicating superparamagnetic behavior. The T B was determined to be 65 K. Notably, the blocking temperature value corroborated well with the reported literature of superparamagnetic iron oxide nanoparticles [36,37]. Such superparamagnetic property has been shown critical to the success application of magnetic nanocapsules in areas such as T 2 -weighted magnetic ressonance imaging, drug delivery and magneto-hyperthermia [38].…”