Development of PCR assays for detection of Escherichia coli O157:H7 in meat products

Application of multiplex PCR assay based on uidR and fliCH7 genes for detection of Escherichia coli O157:H7 in milk

“…Molecular approaches based on PCR assays have also been explored in order to develop sensitive, specific and rapid methods for confirming the presence/absence of E. coli O157: H7. In this context, although a lot of efforts have been made by different investigators to develop PCR-based assays targeted against different genes for detection of E. coli O157:H7 (Cadirci et al, 2010;Cocolin et al, 2000;Gooding and Choudary, 1997;Gordillo et al, 2011;Kawasaki et al, 2010;Park et al, 2011;Paton and Paton, 1998;Rice, 2009), the efficacy of these techniques has been tested with food samples artificially spiked with the target organism. Application of such PCR-based techniques has not yet been efficiently and extensively investigated for detection of E. coli O157:H7 in naturally contaminated dairy foods.…”

Prevalence and characterization of Escherichia coli O157:H7 from samples along the production line in Chinese beef-processing plants

“…All of these primers were commercially synthesized by TaKaRa Biotechnology Co., Ltd. (Dalian, China). The PCR reagent system was used as described by Gordillo, C ordoba, Andrade, Luque, and Rodríguez (2011), with a total volume of 50 mL containing 3 mL of template DNA, 0.2 mM of each primer, 6 U of Taq enzyme, 5 mL of PCR buffer, 3 mM of MgCl 2 , and 0.2 mM each of the dNTPs. A positive control was used during the detection procedure.…”

The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR

“…HUS is considered as the primary reason for acute renal insufficiency among children. The treatment of the disease includesf hemodialysis and supportive care only (7,8).…”