Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus

Abstract

Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to plant quarantine procedures. For this reason, a two-step diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.

Development of PCR-base Diagnostic System for the Detection of Andean potato latent virus

“…및 종자전 염을 하고 Fribourg et al, 1977), 핵산은 3개의 단백질을 암호화하며 총 염기서열의 길이는 약 6.4 kb이다 (Koenig and Lesemann, 1981;Bernal et al, 2000). APLV는 파파리자, 비름과, 명아주과, 박과 및 가지과에서 감염 보고사례 (Gibbs and Harrison, 1969;Koenig and Bode, 1978 (Lee et al, 2013a;Lee et al, 2013c;Lee and Shin, 2014;Lee et al, 2014a;Lee et al, 2015)되어 바이러스 검역실적이 증가하였으나 (Shin, 2009;Lee et al, 2013b) 참 고 문 헌…”

A mini-review on the development and emerging perspectives of seed pathology

“…Unfortunately the method is laden with issues of false positive reaction and low sensitivity (McGee, 1995;Caruso et al, 2003;Priou et al, 2006). As a result, molecular biological methods which are simpler and highly sensitive are at present the preferred choice in seed health testing procedures (Fonseca et al, 2005;Lee et al, 2013;Majumder et al, 2013;Munkvold, 2009;Park and Kim, 2004;Peiró et al, 2011).…”

Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine

“…Second, when more than two kinds of viruses must be examined in the same seed, ELISA and PCR have to be applied separately, thereby wasting labor and manpower, and causing inconvenience in the quarantine site. Third, if the positive control sample group is not secured, PCR test errors and amplification or non-amplification are difficult to accurately assess [13]. Moreover, since the positive control group sample is infected with the virus, it may be difficult to distribute domestically and import [14].…”