Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

Takahashi, Akio; Ogasawara, Kunio; Matsuki, Naoto; Fujinaga, Koh; Nakaya, Takaaki; Ikuta, Kazuyoshi; Auwanit, Wattana; Honda, Mitsuo; Fukui, Yoshihiro; Sasazuki, Takehiko; Iwabuchi, Kazuya; Onoé, Kazunori

doi:10.1016/s0264-410x(98)00042-5

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Recent studies have provided experimental evidence that some synthetic peptides, when conjugated with proper carriers, can mimic the native or recombinant proteins to induce humoral and/or cellular immunity (11, 17). The V3 loop has also been used in these studies and has been successful for inducing neutralizing antibodies and lym-phoproliferative response (14,19). But the impediment to developing a successful V3 loop-based-vaccine lies in the variability of the amino acid sequence of the V3 loop.…”

mentioning

confidence: 99%

Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Bai

Dierich

et al. 2000

Microbiology and Immunology

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confidence: 99%

Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Bai

Dierich

et al. 2000

Microbiology and Immunology

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“…For instance, peptide vaccination has been successfully used to neutralize or protect against viruses (15,21,25,42), bacteria (10), and parasites (22). Based on data presented here, we propose peptide sequences of LF that are excellent candidates for a peptidebased vaccine for anthrax.…”

Section: Discussionmentioning

confidence: 90%

“…Although some B-cell epitopes are conformational in nature, others are comprised of a single continuous stretch of amino acids. The latter category of sequential epitopes is useful in rational design of peptide vaccines, and these epitopes have been demonstrated to elicit neutralizing or protective antibodies to viruses (15,21,25,42), bacteria (10), and parasites (22). Addition of key LF humoral epitopes to a recombinant PA vaccine is expected to increase the titers of LeTx-neutralizing antibodies in the sera of vaccinees and thus provide enhanced efficacy.…”

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confidence: 99%

Sequential B-Cell Epitopes ofBacillus anthracisLethal Factor Bind Lethal Toxin-Neutralizing Antibodies

et al. 2009

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The bipartite anthrax lethal toxin (LeTx) consisting of protective antigen (PA) and lethal factor (LF) is a major virulence factor contributing to death from systemic Bacillus anthracis infection. The current vaccine elicits antibodies directed primarily to PA; however, in experimental settings serologic responses to LF can neutralize LeTx and contribute to protection against infection. The goals of the present study were to identify sequential B-cell epitopes of LF and to determine the capacity of these determinants to bind neutralizing antibodies. Sera of recombinant LF-immunized A/J mice exhibited high titers of immunoglobulin G anti-LF reactivity that neutralized LeTx in vitro 78 days after the final booster immunization and protected the mice from in vivo challenge with 3 50% lethal doses of LeTx. These sera bound multiple discontinuous epitopes, and there were major clusters of reactivity on native LF. Strikingly, all three neutralizing, LF-specific monoclonal antibodies tested bound specific peptide sequences that coincided with sequential epitopes identified in polyclonal antisera from recombinant LF-immunized mice. This study confirms that LF induces high-titer protective antibodies in vitro and in vivo. Moreover, the binding of short LF peptides by LF-specific neutralizing monoclonal antibodies suggests that generation of protective antibodies by peptide vaccination may be feasible for this antigen. This study paves the way for a more effective anthrax vaccine by identifying discontinuous peptide epitopes of LF.

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Prevention of infection of influenza virus in DQ6 mice, a human model, by a peptide vaccine prepared according to the cassette theory

Matsuki

Ogasawara

Takami

et al. 1999

Vaccine

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Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

Cited by 6 publications

References 23 publications

Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Sequential B-Cell Epitopes ofBacillus anthracisLethal Factor Bind Lethal Toxin-Neutralizing Antibodies

Prevention of infection of influenza virus in DQ6 mice, a human model, by a peptide vaccine prepared according to the cassette theory

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