Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Yu, Tao; Bai, Yun; Dierich, Manfred P.; Chen, Yinghua

doi:10.1111/j.1348-0421.2000.tb01253.x

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…The anti-SEAGepis antibody was not only highly specific to SEA and SEG but also had a very high titer (1:819,200), which was much greater than that induced by the complete SEA or SEG antigen (1:16,384, data not shown). The finding is similar to those previously reported for multiepitope peptides (25,26). This is not surprising because the epitope peptide, a special type of peptide comprising several predefined epitopes without other sequences, could enhance the immunogenicity and reactogenicity of these epitopes.…”

Section: Discussionsupporting

confidence: 91%

Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on the Multiepitope Peptide for the Synchronous Detection of Staphylococcal Enterotoxin A and G Proteins in Milk

Liang

Zhang

Liu

et al. 2015

Journal of Food Protection

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Staphylococcal food poisoning (SFP), one of the most common foodborne diseases, results from ingestion of staphylococcal enterotoxins (SEs) in foods. In our previous studies, we found that SEA and SEG were two predominant SE proteins produced by milkacquired S. aureus isolates. Here, a tandemly arranged multiepitope peptide (named SEAGepis) was designed with six linear B-cell epitopes derived from SEA or SEG and was heterologously expressed. The SEAGepis-specific antibody was prepared by immunizing rabbit with rSEAGepis. Then, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on rSEAGepis and the corresponding antibody was developed to simultaneously detect SEA and SEG. Under the optimized conditions, the ic-ELISA standard curve for rSEAGepis was constructed in the concentration range of 0.5 to 512 ng/ml, and the average coefficients of variation of intra- and interassay were 4.28 and 5.61% during six standard concentrations. The average half-maximal inhibitory concentration was 5.07 ng/ml, and the limit of detection at a signal-to-noise ratio of 3 was 0.52 ng/ml. The anti-rSEAGepis antibody displayed over 90% cross-reactivity with SEA and SEG but less than 0.5% cross-reactivity with other enterotoxins. Artificially contaminated milk with different concentrations of rSEAGepis, SEA, and SEG was detected by the established ic-ELISA; the recoveries of rSEAGepis, SEA, and SEG were 91.1 to 157.5%, 90.3 to 134.5%, and 89.1 to 117.5%, respectively, with a coefficient of variation below 12%. These results demonstrated that the newly established ic-ELISA possessed high sensitivity, specificity, stability, and accuracy and could potentially be a useful analytical method for synchronous detection of SEA and SEG in milk.

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Section: Discussionsupporting

confidence: 91%

Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on the Multiepitope Peptide for the Synchronous Detection of Staphylococcal Enterotoxin A and G Proteins in Milk

Liang

Zhang

Liu

et al. 2015

Journal of Food Protection

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“…This still did not completely exclude the substantial possibility that the carrier protein itself may result in a stronger immune reaction than the intended antigen, thereby resulting in little or no production of Ab against the specific peptide. Subsequently, an alternative idea was presented in which a macro-molecule was assembled by repetitive linkage of small specific peptides to generate a strong and efficient Ab response (Astori & Kraehenbuhl 1996;Yu et al 2000;Schroeder et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Production of an epitope-specific antibody using recombinant repetitive oligonucleotides

Park

Kim

Seo

et al. 2014

Animal Cells and Systems

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This study was performed to produce a strong antibody against repeated peptides for a specific region of an antigen. Nine amino acids at C-terminal from human brain β-tubulin III were selected as a target sequence since it is well known for a high conservancy of sequence and resistance to antibody production. The synthetic oligonucleotides had six bases (5′-CGAC-GT-3′, 5′-TC-CAGC-3′) appended at each end and were repeatedly linked and directly joined by ligase in the presence of restriction enzymes (AccI, AvaI) to allow for the construction of head-to-tail repeats. Ligated oligonucleotides containing more than 10-unit repeats were eluted from agarose gels and cloned into the AccI site of a modified pGEX expression vector. The cloned repeated target sequences were subsequently expressed in a bacterial system. A large amount of glutathione-Stransferase-fusion protein containing the unit repeats was purified by affinity chromatography on glutathioneagarose beads and injected into Balb/c mice to produce polyclonal and monoclonal antibodies (Abs). Total protein from several mammalian brains was extracted and utilised to test the quality of the generated Abs in immunoblot assays. The generated Abs specifically recognised the 55-kDa human brain β-tubulin protein but not any other mammalian brain β-tubulins. The potential uses of this system could be applied to an effective epitope generation for an antibody and to the development of a new type of subunit vaccine.

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Induction of multi-epitopespecific antibodies against HIV-1 by multi-epitopevaccines

Ding

et al. 2001

Chin.Sci.Bull.

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Induction of High Levels of Epitope‐Specific Antibodies by Epitope/Peptide Candidate Vaccines against Human Immunodeficiency Virus Type‐1 (HIV‐1)

Abstract: To test the immunogenicity of GPGRAFY-epitope-based candidate vaccines, a peptide with four repetitive GPGRAFY epitopes,

Cited by 9 publications

References 19 publications

Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on the Multiepitope Peptide for the Synchronous Detection of Staphylococcal Enterotoxin A and G Proteins in Milk

Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on the Multiepitope Peptide for the Synchronous Detection of Staphylococcal Enterotoxin A and G Proteins in Milk

Production of an epitope-specific antibody using recombinant repetitive oligonucleotides

Induction of multi-epitopespecific antibodies against HIV-1 by multi-epitopevaccines

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