Development of plasmid and oligonucleotide nanometric particles

Dauty, Emmanuel; Jp, Behr; Js, Remy

doi:10.1038/sj.gt.3301759

Cited by 27 publications

(23 citation statements)

References 17 publications

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“…[17][18][19] First, pDNA molecules were individually condensed with a cationic cysteine-based detergent and then stabilized by oxidative dimerization of the detergent. Condensation using tetradecane-based detergent produced particles that exhibited efficient cell transfection activity.…”

Section: Discussionmentioning

confidence: 99%

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Development of Small, Homogeneous pDNA Particles Condensed with Mono-cationic Detergents and Encapsulated in a Multifunctional Envelope-type Nano Device

Suzuki

Yamada

Harashima

2008

Biological & Pharmaceutical Bulletin

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Non-viral DNA vectors are promising gene delivery systems and a variety of non-viral DNA vectors have been developed to date. Recently, we developed a novel non-viral gene delivery system-multifunctional envelope-type nano device (MEND). The MEND system has high transfection activity, similar to that of adenovirus vector, which is a potent viral vector. However, conventional MEND is relatively large and heterogeneous (approximately 300 nm), probably because they contain relatively large-and heterogeneous-pDNA particles condensed with polycations, such as poly-L-lysine. Small particle size is important for in vivo delivery, because large particles are rapidly eliminated from systemic circulation. Moreover, heterogeneous size of drug carriers is difficult to apply to clinical applications. Here, we describe construction of small homogeneous MEND. First, we screened mono-cationic detergents (MCD(s)) to obtain optimal pDNA condensed particles. We determined that benzyldimethylhexadecylammonium chloride (BDHAC) and thonzonium bromide (TB) were optimal pDNA condensers. Next, we packaged the condensed pDNA particles into a lipid bi-layer. The resulting lipid-encapsulated pDNA particles were then equipped with octaarginine to facilitate cell-uptake (R8-MEND (MCD)). The carrier showed high transfection activity in cultured HeLa cells. Furthermore, the R8-MEND (MCD) were small and homogeneous compared with conventional MEND. These results indicate that R8-MEND (MCD) has potential as a novel non-viral delivery system for clinical application.

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Section: Discussionmentioning

confidence: 99%

“…15,16) Behr and coworkers developed stabilized monomolecular pDNA nano-particles using a two-step process. [17][18][19] First, pDNA molecules were individually condensed using a cationic cysteine-based detergent. The resulting particles were then stabilized by oxidative dimerization of the detergent into a Gemini lipid on the template pDNA.…”

mentioning

confidence: 99%

Development of Small, Homogeneous pDNA Particles Condensed with Mono-cationic Detergents and Encapsulated in a Multifunctional Envelope-type Nano Device

Suzuki

Yamada

Harashima

2008

Biological & Pharmaceutical Bulletin

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“…Oligonucleotide particles prepared in this manner have a size range of 50-500 nm. 10,12 Cationic amphiphiles are widely used as effective tools in delivery of DNA into mammalian cells. 3 Block copolymers containing polyethyleneglycol (PEG) have been used in various drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Dendrosomes as novel gene porters‐III

Majid¹,

Ranjbar²,

Damaghi³

et al. 2008

J of Chemical Tech & Biotech

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“…Recently, there have been substantial advances in the application of nanomaterials, especially bioceramics, for cancer diagnostics and as carriers for delivering small therapeutics molecules. [9][10][11][12] There are many critical features of these bioceramics nanoparticles that enable them to function as ideal DNA carriers. 13,14 One could easily modify the particle size, stability, and loading capacity of these particles by customizing the structures and physicochemical properties to ensure their effectiveness and safety for clinical applications.…”

Section: Introductionmentioning

confidence: 99%