Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies

Maciel, Marilene Oliveira dos Santos; Soares, Matheus Fujimura; Costa, Sidnei Ferro; Bragato, Jaqueline Poleto; Freitas, Jéssica Henrique de; Venturin, Gabriela Lovizutto; Melo, Larissa Martins; Rebech, Gabriela Torres; Reed, Steve; Lima, Valéria Marçal Felix de

doi:10.1016/j.jim.2019.112664

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…Many antigens have been assessed for antibody detection, and recombinant antigens are preferred over natural antigens, as the latter often cause problems such as cross-reactivity and resultant false-positive results [ 61 , 96 ]. Some immunoassays, such as enzyme-linked immunosorbent assay (ELISA) and western blot, require relatively sophisticated and expensive equipment and materials, which renders them less useful in endemic regions in poorer countries, despite their good sensitivities and robustness [ 97 , 98 ]. However, ELISA is widely used as a serological method in countries where leishmaniasis is endemic or non-endemic, and it can provide detailed information on antibody responses [ 99 , 100 ].…”

Section: Methods For the Detection And Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions. Transmitted by the bite of a female sand fly, Leishmania infections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species of Leishmania that infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection of Leishmania have been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods. Graphical abstract

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Section: Methods For the Detection And Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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“…However, the high diagnostic performance of rK28 reported here corroborates the high levels of sensitivity (96%) and specificity (99%) of a rK28-based ELISA developed and evaluated by [ 36 ] using classical validation approaches. The higher diagnostic performance of an rK28 antigen-based plasmonic ELISA compared to DPP-LVC ® has also been reported recently [ 37 ]. The usefulness of this ELISA, whose results can be read with the naked eye, in the serodiagnosis of canine Leishmania infection, whether in epidemiological studies or clinical practice, deserves further validation.…”

Section: Discussionmentioning

confidence: 76%

“…An alternative to the Leishmania infantum IgG ELISA ® that is not available in the Brazilian market is the use of recombinant antigens in ELISA [ 5 ], such as the rK28-based ELISA mentioned above [ 36 , 37 ]. This alternative would ease the screening of large numbers of samples at a lower cost than IRTs and would minimize the undesirable culling of false-positive dogs.…”

Section: Discussionmentioning

confidence: 99%

Leishmania exposure in dogs from two endemic countries from New and Old Worlds (Brazil and Portugal): evaluation of three serological tests using Bayesian Latent Class Models

Maia¹,

Fraga

Cristóvão³

et al. 2022

Parasites Vectors

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Background Zoonotic leishmaniosis caused by Leishmania infantum is endemic in several countries of the Mediterranean Basin, Latin America, and Asia. Dogs are the main hosts and reservoirs of human infection. Thus, from a One Health perspective, early diagnosis of Leishmania infection in dogs is essential to control the dissemination of the parasite among other dogs and to humans. The aim of this study was to estimate the diagnosis accuracy of three serological tests to detect antibodies to Leishmania in dogs from two endemic settings using Bayesian latent class models (BLCM). Methods A total of 378 dogs from two Portuguese and Brazilian endemic areas of leishmaniosis (194 animals from Portugal and 184 from Brazil) were screened. Detection of anti-Leishmania antibodies was performed using two commercial ELISA (L. infantum IgG-ELISA® and EIE-LVC®) and a rapid immunochromatographic test (DPP-LVC®). Bayesian latent class models were used to estimate Leishmania infection prevalence, together with sensitivities and specificities of the three diagnostic tests, in the two dog populations simultaneously. Predictive values were also calculated. Credibility intervals (CI) were obtained, considering different types of prior information. Results A posterior median Leishmania seroprevalence of 13.4% (95% CI 9.0–18.7) and of 21.6% (15.0–28.3) was estimated to the Portuguese and Brazilian dog subpopulations, respectively. The Bayesian analysis indicated that all tests were highly specific (specificity above 90%), and that the DPP-LVC® was more sensitive (96.6%; 83.1–99.9) than both ELISAs in the Portuguese subpopulation, while in the Brazilian subpopulation, EIE-LVC® and L. infantum IgG-ELISA®, had the highest sensitivity (88.2%; 73.7–97.0) and specificity (98.7%; 95.1–99.9), respectively. Conclusions In general, the levels of diagnosis accuracy of the three serological tests to detect Leishmania antibodies assessed by BLCM indicate their utility in canine epidemiological studies. The same approach should be used to assess the performance of these techniques in the clinical management of infected and sick dogs using representative samples from the wide spectrum of clinical situations, namely from subclinical infection to manifest disease. The low positive predictive value of the serological tests used in the current protocol of the Brazilian Ministry of Health suggests that they should not be used individually and may not be sufficient to target reservoir-based control interventions. Graphical Abstract

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“…The canine serum samples were tested by pELISA with modifications to detect anti‐rK28 IgG antibodies with the naked eye . Briefly, the pELISA used a microtiter plate (1/2 area; Corning Inc., Cat.…”

Section: Methodsmentioning

confidence: 99%

“…Infection. We observed that the pELISA facilitated detection with the naked eye and may dispense with the use of sophisticated instruments to read the results …”

Section: Introductionmentioning

confidence: 99%

Plasmonic rK28 ELISA improves the diagnosis of canineLeishmaniainfection

Maciel

Soares

Costa

et al. 2019

Parasite Immunology

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In this study, we evaluated the performance of a new enzyme‐linked immunosorbent assay (ELISA) variant known as indirect “plasmonic ELISA” (pELISA) for the detection of Leishmania spp. infection. Serum samples from 170 dogs from an area where canine leishmaniosis (CanL) is endemic and from 26 healthy dogs from a nonendemic area were tested by indirect pELISA, and the results were compared to those of an indirect ELISA (both with recombinant antigen rK28) and those of an immunochromatographic test (dual‐path platform, TR‐DPP®) using real‐time PCR on blood samples or conjunctival swabs as the gold standard. The pELISA, indirect rK28 ELISA and the TR‐DPP® immunochromatographic test presented sensitivities of 94.7%, 89.5% and 79.0% and specificities of 100%, 92.7% and 91.5%, respectively. The analysis of the results revealed that the specificity of the indirect pELISA was greater than that of the method recommended by the Ministry of Health in Brazil and may increase the feasibility of diagnosis in resource‐constrained countries because it does not require sophisticated instruments to read. Thus, this method can be used as an additional tool for the detection of Leishmania spp. infection in these areas.

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Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies

Cited by 12 publications

References 18 publications

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Leishmania exposure in dogs from two endemic countries from New and Old Worlds (Brazil and Portugal): evaluation of three serological tests using Bayesian Latent Class Models

Plasmonic rK28 ELISA improves the diagnosis of canineLeishmaniainfection

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