Development of Poly(2-Methacryloyloxyethyl Phosphorylcholine)-Functionalized Hydrogels for Reducing Protein and Bacterial Adsorption

Vales, Temmy Pegarro; Jee, Jun‐Pil; Lee, Won Young; Cho, Sung June; Lee, Gye Myung; Kim, Ho‐Joong; Kim, Jung Suk

doi:10.3390/ma13040943

Cited by 26 publications

(26 citation statements)

References 67 publications

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“…Overall, MPC demonstrated a significant antifouling effect for both proteins: for the hydrogels with the highest value of MPC, lower thickness and Δm values were obtained. This is in agreement with previous studies that found that the existence of zwitterionic MPC moieties on the surfaces of the HEMA- and silicone-based hydrogels decreased the adsorption of proteins, such as lysozyme, fibrinogen and albumin [ 56 , 57 , 58 ]. This protein repulsion capacity was attributed to the high hydration of the phosphorylcholine groups present on MPC [ 56 , 58 ].…”

Section: Resultssupporting

confidence: 93%

“…This may be due to the small amount of MPC, whose effect is minor when compared to that induced by the other hydrophilic monomers present in the hydrogel matrix. Other authors found a reduction in the water contact angle with an increase in MPC amount, but for hydrogels with MPC grafted to the surface [ 56 , 57 ] or with much higher amounts of MPC [ 58 ]. It should be noted that the contact angles obtained for all compositions fell in the range of the values typical of commercial CLs and were similar to the contact angle reported for Proclear ® (47.4 ± 7.5°) [ 59 ] using the same technique.…”

Section: Resultsmentioning

confidence: 99%

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Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

et al. 2021

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Contact lenses (CLs) are prone to biofilm formation, which may cause severe ocular infections. Since the use of antibiotics is associated with resistance concerns, here, two alternative strategies were evaluated to endow CLs with antibiofilm features: copolymerization with the antifouling monomer 2-methacryloyloxyethyl phosphorylcholine (MPC) and loading of the antioxidant resveratrol with known antibacterial activity. MPC has, so far, been used to increase water retention on the CL surface (Proclear® 1 day CLs). Both poly(hydroxyethyl methacrylate) (HEMA) and silicone hydrogels were prepared with MPC covering a wide range of concentrations (from 0 to 101 mM). All hydrogels showed physical properties adequate for CLs and successfully passed the hen's egg-chorioallantoic membrane (HET-CAM) test. Silicone hydrogels had stronger affinity for resveratrol, with higher loading and a slower release rate. Ex vivo cornea and sclera permeability tests revealed that resveratrol released from the hydrogels readily accumulated in both tissues but did not cross through. The antibiofilm tests against Pseudomonas aeruginosa and Staphylococcus aureus evidenced that, in general, resveratrol decreased biofilm formation, which correlated with its concentration-dependent antibacterial capability. Preferential adsorption of lysozyme, compared to albumin, might also contribute to the antimicrobial activity. In addition, importantly, the loading of resveratrol in the hydrogels preserved the antioxidant activity, even against photodegradation. Overall, the designed hydrogels can host therapeutically relevant amounts of resveratrol to be sustainedly released on the eye, providing antibiofilm and antioxidant performance.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

et al. 2021

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“…Contact lenses, either poly-HEMA or SiHy, surface-functionalization with poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC) have enhanced surface wettability [468] and water content retention, whilst lowering the adsorption of proteins such as lysozyme and albumin and bacterial binding compared to unmodified control lenses [469,470]. The anti-biofouling properties of the pMPC-functionalised hydrogels were attributed to the dense hydration layer formed at the hydrogel surfaces by the zwitterionic coating [469,471]. The surface coating did not affect the oxygen permeability and modulus [470].…”

Section: Reducing Deposition Of Tear Film Components To Contact Lensesmentioning

confidence: 99%

BCLA CLEAR - Contact lens wettability, cleaning, disinfection and interactions with tears

Willcox

Keir

Maseedupally

et al. 2021

Contact Lens and Anterior Eye

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Contact lens materials have undergone significant changes over the past 20 years, particularly with respect to the introduction of silicone hydrogel materials. Whilst this development addressed hypoxic issues, other important areas relating to contact lens success, notably comfort, require further research.Contact lens wettability remains a crucially important part of biocompatibility. Contact lenses can be made more wettable by incorporation of surfactants into blister packs, internal wetting agents, surface treatments or care solutions. However, there remains no clear association between contact lens wettability and comfort, making it challenging to determine the potential for these approaches to be of significant clinical benefit. Most contact lenses are used on a daily wear, reusable basis, which requires them to be disinfected when not worn. The ideal disinfecting solution would also improve comfort during wear. However, balancing these requirements with other factors, including biocompatibility, remains a challenge. Soft lens materials invariably take up and subsequently release certain components of disinfecting solutions onto the ocular surface. This may affect tear film stability and the normal ocular microbiome, and further research is needed in this area to determine whether this has any affect on comfort. Finally, contact lens materials sorb components of the tear film, and these interactions are complex and may change the biochemistry of the tear film, which in turn may affect their comfort.In conclusion, the interaction between lens materials, tear film and disinfection solution plays an important role in the biocompatibility of lenses. However, the exact role and whether this can be altered to improve biocompatibility and comfort during wear remains debatable. This report summarises the best available evidence to examine this complex relationship and the opportunities for practitioners to enhance in-eye comfort of contemporary lenses, along with providing suggestions for areas of study that may provide further information on this topic.

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“…Zwitterionic materials are a class of materials that contain both cationic and anionic groups, with no overall charge (neutral). Zwitterionic materials can be divided into the following categories according to the monomer structural characteristics (as shown in Figure 3 a): (1) phosphobetaine with a phosphate group and a terminal quaternary ammonium group [ 21 , 22 , 23 , 24 ]; (2) sulfobetaine or carboxybetaine with a quaternary ammonium group and a terminal carboxyl or sulfonic group [ 25 , 26 , 27 , 28 ]; (3) zwitterionic polyampholytes [ 29 ]. For the first two kinds of materials, each side-chain unit in the polymer contains an equal amount of positive and negative charges, which is electrically neutral.…”

Section: Structure Of Zwitterionic Materialsmentioning

confidence: 99%

Recent Advances in Zwitterionic Hydrogels: Preparation, Property, and Biomedical Application

Liu

Tang

et al. 2022

Gels

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Nonspecific protein adsorption impedes the sustainability of materials in biologically related applications. Such adsorption activates the immune system by quick identification of allogeneic materials and triggers a rejection, resulting in the rapid failure of implant materials and drugs. Antifouling materials have been rapidly developed in the past 20 years, from natural polysaccharides (such as dextran) to synthetic polymers (such as polyethylene glycol, PEG). However, recent studies have shown that traditional antifouling materials, including PEG, still fail to overcome the challenges of a complex human environment. Zwitterionic materials are a class of materials that contain both cationic and anionic groups, with their overall charge being neutral. Compared with PEG materials, zwitterionic materials have much stronger hydration, which is considered the most important factor for antifouling. Among zwitterionic materials, zwitterionic hydrogels have excellent structural stability and controllable regulation capabilities for various biomedical scenarios. Here, we first describe the mechanism and structure of zwitterionic materials. Following the preparation and property of zwitterionic hydrogels, recent advances in zwitterionic hydrogels in various biomedical applications are reviewed.

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Development of Poly(2-Methacryloyloxyethyl Phosphorylcholine)-Functionalized Hydrogels for Reducing Protein and Bacterial Adsorption

Cited by 26 publications

References 67 publications

Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

BCLA CLEAR - Contact lens wettability, cleaning, disinfection and interactions with tears

Recent Advances in Zwitterionic Hydrogels: Preparation, Property, and Biomedical Application

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