Timber floors suffering from poor serviceability performance can be upgraded with a concrete topping to form a timber-concrete composite. The upgrade stiffens the floor, reducing the perception of a vibration response to dynamic excitation. Despite timber-concrete composites becoming an established research area in recent years, relatively little is known about the vibration response of these floor types. This paper explores how the vibration response of a timber floor changes when upgraded with a concrete topping, with particular attention given to the fundamental frequency of vibration. An analytical model, utilising the gamma method of Eurocode 5 (EN 1995-1-1), is used to predict how the fundamental frequency of vibration changes with the addition of a topping.The model is compared with experimental testing of timber-concrete panels before it is used to conduct a parametric study to establish the effect of common factors. It is found that high interaction between the topping and timber floor, identifying a suitable topping thickness and considering the change in transverse stiffness are key to a successful upgrade. It is suggested that topping upgrades which are thin (20 mm or less) are suitable for this application.