Development of primer and probe sets for the detection of plant species in honey

Laube, Ines; Hird, Heather; Brodmann, Peter; Ullmann, Susanne; Schöne-Michling, M.; Chisholm, James; Broll, Hermann

doi:10.1016/j.foodchem.2008.09.063

Cited by 102 publications

(68 citation statements)

References 5 publications

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“…Una opción para identificar las especies vegetales presentes en la miel, son los métodos analíticos basados en el ADN; adecuados para la normalización como base para la armonización en la Unión Europea y otros países (18) . En este sentido, la definición del origen botánico de las mieles con fines de proporcionar un valor agregado al producto, establece la necesidad de contar con un sistema de detección de secuencias nucleotídicas homólogas, empleando oligonucleótidos genéricos derivados de genes metabólicos que, si bien son afines a todas las especies, también tienen patrones de amplificación muy particulares entre familias de plantas.…”

Section: Methodsunclassified

“…La extracción de ADN de miel se realizó empleando 50 g de miel por cada muestra distribuyéndose Primers. Seven previously published consensus sequence primers were used to establish plant species amplification patterns (18) , and then to identify these amplification patterns and their dissociation curves in the honey samples: 1) non-coding tRNA-Leu chloroplastic DNA sequence of trnL (UAA) intron, consensus of 19 species including algae, bryophytes, pteridophytes, gymnosperms and angiosperms (18,21) (Plant 1); 2) nested Plant 1 sequence (Plant nest) (18) ; 3) canola and sunflower actin (Act) (18) ; 4) sunflower profilin (Helli-all) (18) ; 5) canola lipase (Brass-lip) (18) ; 6) sorghum, rice and rye alcohol dehydrogenase (Adh1) (18) ; and 7) cotton, pepper, tomato, potato and tobacco 3-hydroxymethyl glutaryl CoA (Hmg2) (18) .…”

Section: Materials Y Métodosmentioning

confidence: 99%

“…An effective method for identifying the botanical source are those based on DNA, suitable for the honey quality regulations used for standardization within the European Union and other countries (18) . Defining honey botanical sources with the aim of adding value to the final product requires detection of homologous nucleotide sequences using generic oligonucleotides derived from metabolic genes.…”

Section: Introductionmentioning

confidence: 99%

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Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

Cázares¹,

Ordóñez

Cruz

et al. 2016

Rev. Mex. Cienc. Pecu.

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RESUMENEl método cuantitativo de reacción en cadena de la polimerasa (qPCR), seguido por análisis de curvas de disociación, fue desarrollado para la detección rápida y simultánea de la composición botánica en mieles de la Península de Yucatán, México. Cinco muestras de miel ciclo apícola 2013 y cinco del 2014, se caracterizaron para definir el contenido de cinco especies de plantas de importancia apícola; Viguiera dentata, Gymonopodium floribundum, Piscidia piscipula, Acacia angustissima y Mimosa bahamensis. These results correlate to melissopalynological analysis for most cases. P. piscipula was not detected in any honey sample; however, according to melissopalynological analysis A. angustissima was present in M-3 and M-4 even though it was unable to detect it, possibly due to a low or no similarity with generic genes sequence.

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Section: Methodsunclassified

Section: Materials Y Métodosmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

Cázares¹,

Ordóñez

Cruz

et al. 2016

Rev. Mex. Cienc. Pecu.

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“…3). To confirm the presence of amplifiable DNA, a PCR test can be used that amplifies a plantspecific DNA sequence, such as the β-actin gene (ACT) (Laube et al, 2010), chloroplast DNA, or nuclear ribosomal small subunit genes (such as 18S). To confirm the presence of maize, the following genes can be used: the high-mobility group gene (hmg), the alcohol dehydrogenase-1 (Adh1) gene, the maize starch synthase gene (zSSIIb), and the invertase gene (ivr1) (Broothaerts et al, 2008;Scholdberg et al, 2008).…”

Section: B) Screening and Quantification Of Gmosmentioning

confidence: 99%

Pollen from genetically modified plants in honey – problems with quantification and proper labelling

Żmijewska¹,

Teper²,

Linkiewicz³

et al. 2013

Journal of Apicultural Science

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a b s t r a c t maize can be a valuable source of pollen when plants attractive for bees are not available. honeybees can forage from conventional maize as well as from genetically modified (gm) maize. the court of Justice of the european union (eu) ruled that pollen in honey must be treated as a food ingredient and therefore falls within the scope of regulation 1829/2003/ec on gm food and feed and requires authorization. gm pollen unauthorized in the eu cannot be present in honey at any level, and honey must be labelled if it contains more than 0.9% of pollen from authorized gm plants in relation to total pollen content. however, currently available analytical methods allow only for estimation of gm pollen quantity in honey. therefore, directive 2001/110/ec related to honey needs to be amended so that pollen can be regarded as a natural constituent of honey. because the eu is a big honey importer, validated and harmonized detection methods are necessary for the control of gm pollen in honey.

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“…The present study aims to develop a simple and efficient method for the extraction of PCR amplifiable chloroplast DNA (cpDNA) with a reduced sample amount of 3 ml honey compared with previous studies (e.g., 10 ml by Cheng et al 6 and 10 g by Laube et al 7 ). As other honey DNA isolation methods are mostly described as kit-based approaches, we developed an easy and efficient method for honey DNA isolation by conventional phenol-chloroform methods.…”

Section: Introductionmentioning

confidence: 99%

Protocol for Optimal Quality and Quantity Pollen DNA Isolation from Honey Samples

Lalhmangaihi¹,

Ghatak²,

Laha³

et al. 2014

J Biomol Tech

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The present study illustrates an optimized sample preparation method for an efficient DNA isolation from low quantities of honey samples. A conventional PCR-based method was validated, which potentially enables characterization of plant species from as low as 3 ml bee-honey samples. In the present study, an anionic detergent was used to lyse the hard outer pollen shell, and DTT was used for isolation of thiolated DNA, as it might facilitate protein digestion and assists in releasing the DNA into solution, as well as reduce cross-links between DNA and other biomolecules. Optimization of both the quantity of honey sample and time duration for DNA isolation was done during development of this method. With the use of this method, chloroplast DNA was successfully PCR amplified and sequenced from honey DNA samples.

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Development of primer and probe sets for the detection of plant species in honey

Cited by 102 publications

References 5 publications

Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

Pollen from genetically modified plants in honey – problems with quantification and proper labelling

Protocol for Optimal Quality and Quantity Pollen DNA Isolation from Honey Samples

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