Development of pyruvate oxidase-based amperometric biosensor for pyruvate determination

Knyzhnykova, D. V.; Topolnikova, Ya. V.; Кучеренко, І. С.; Солдаткін, О. О.

doi:10.7124/bc.00096c

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…However, this range was lower than the wide concentration range of pyruvate biosensor based on enzyme nanoparticles of POx (Malik et al, 2019). The LOD was calculated to be 0.72 µM, which is far better than most of the earlier described biosensors available till date Yorganci, 2008, Li et al, 2017;Berntsson, 1955;Gajovic et al, 2006;Maines et al, 2000;Knyzhnykova et al, 2018;Mizutani et al, 1980). This LOD was not lower than that of pyruvate biosensor based on POxNPs/AuE (Malik et al, 2019).…”

Section: Evaluation Of Biosensormentioning

confidence: 75%

Construction of an Amperometric Pyruvate Biosensor Based on Enzyme Bound to A Nanocomposite and Its Comparison with Enzyme Nanoparticles Bound to Electrode

Malik

Chaudhary

Pundir

2019

Int J Appl Sci Biotechnol

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An improved amperometric pyruvate biosensor was fabricated by immobilizing covalently commercial pyruvate oxidase (POx) from Aerococcus sps. onto nanocomposite of c-MWCNT (carboxylated multi- walled carbon nanotubes), copper nanoparticles (CuNPs) and polyaniline (PANI) electrodeposited onto gold (Au) electrode. The copper nanoparticles were prepared by chemical reduction method and characterized by transmission electron microscopy (TEM), UV- visible spectroscopy and X- ray diffraction (XRD). The working electrode (POx/c-MWCNT/CuNPs/PANI/AuE was studied via scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and electrochemical impedance spectroscopy (EIS) at different stages of its construction. The biosensor showed optimum activity at a pH of 5.0 and 35°C and a linearity for pyruvate in the concentration range, 0.1 µM to 2000 µM. The analytical recovery of added pyruvate was 99.6% and 99.2%. The within and between batch coefficients of variation (CV) were 0.052% and as 0.022% respectively. There was a commendable correlation between sera pyruvate values as measured by standard spectrophotometric method and the present method. The biosensor was applied to measure sera pyruvate level and compared with that biosensor based on pyruvate oxidase nanoparticles covalently bound to Au electrode. Malik et al. (2019) Int. J. Appl. Sci. Biotechnol. Vol 7(2): xxx-xxx Graphic Abstract Schematic representation of fabrication of POx/c-MWCNTs/CuNPs/PANI/AuE and electrochemical reactions involved in its response measurement (POx- Pyruvate oxidase; c-MWCNTs- Carboxylated multi-walled carbon nanotubes; CuNPs- Copper nanoparticles; PANI- Polyaniline; AuE- Gold electrode).

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Section: Evaluation Of Biosensormentioning

confidence: 75%

Construction of an Amperometric Pyruvate Biosensor Based on Enzyme Bound to A Nanocomposite and Its Comparison with Enzyme Nanoparticles Bound to Electrode

Malik

Chaudhary

Pundir

2019

Int J Appl Sci Biotechnol

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“…Notable, POx requires additional substances for the pyruvate oxidation (phosphates, Mg 2+ and TPP). Concentrations of these substances in the working buffer (20 mM, 125 μM, and 500 μM, respectively) were previously optimized .…”

Section: Resultsmentioning

confidence: 99%

Novel Multiplexed Biosensor System for the Determination of Lactate and Pyruvate in Blood Serum

et al. 2019

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Multiplexing is one of the main current trends in biosensors, especially important for clinical diagnosis. However, simultaneous determination of several substances in one sample is often difficult due to different performance and working conditions of separate biosensors. This work was aimed at the development of a multiplexed biosensor system for the determination of lactate and pyruvate concentrations in liquid samples (i. e., blood serum). The system consisted of two amperometric biosensors based on lactate oxidase and pyruvate oxidase, which worked simultaneously in a single measuring cell. Conditions for the biosensor system work were selected. Linear range of lactate determination was 5–1000 μM, pyruvate – 10–5000 μM. Steady‐state response time was 30 s and 50 s for the lactate and pyruvate biosensors, respectively. After 2 weeks of storage biosensor responses decreased to 95 % (lactate biosensor) or 82 % (pyruvate biosensor) of the initial value. A scheme of analysis of the concentrations of lactate and pyruvate in human blood serum was proposed. The lactate and pyruvate concentrations as well as their ratio in human blood serum samples were determined and compared with the control method. The proposed biosensor system is suitable for the rapid detection of lactate, pyruvate and their ratio and can be used for clinical diagnosis, e. g., evaluation of the reasons of lactic acidosis and prognosis of patient's recovery.

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“…Moreover, the easiness to miniaturize, and the low cost associated with detection methods made electrochemical sensing as most favorable modality for detection of biomolecules. Recently, an amperometric detection strategy has been developed for the rapid detection of pyruvate in body fluids, where pyruvate oxidase (POx) was used, which acted as a recognition element for biomolecule and platinum disc as a transducer element [9]. Also pyruvate sensing platform using covalent immobilization of pyruvate oxidase onto polytriamine modified glassy carbon disc electrode has been developed with a detection limit of 0.05 mM [10].…”

Section: Introductionmentioning

confidence: 99%

A Non‐enzymatic Disposable Electrochemical Sensor for Pyruvic Acid

Swetha

Prasad

2020

Electroanalysis

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Pyruvic acid (PA), plays an important role in metabolic pathway of living organism, and found use in health care, food, drug and agro-chemical industries. Herein, we report development of disposable silver nanoparticles decorated reduced graphene oxide (AgÀ rGO) nanocomposite electrodes for non-enzymatic detection of PA.The AgÀ rGO endowed enhanced electrochemical active area, and exhibited excellent electrochemical activity towards PA with well-defined oxidation peak at 400 mV. The sensor exhibited a linear range of 0.025-1.43 μM for PA with detection limit of 0.5 nM. The validation of developed PA sensor was performed with onion juice and, reason behind electrode fouling affect on electrochemical oxidation of PA also investigated.

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Development of pyruvate oxidase-based amperometric biosensor for pyruvate determination

Cited by 10 publications

References 9 publications

Construction of an Amperometric Pyruvate Biosensor Based on Enzyme Bound to A Nanocomposite and Its Comparison with Enzyme Nanoparticles Bound to Electrode

Construction of an Amperometric Pyruvate Biosensor Based on Enzyme Bound to A Nanocomposite and Its Comparison with Enzyme Nanoparticles Bound to Electrode

Novel Multiplexed Biosensor System for the Determination of Lactate and Pyruvate in Blood Serum

A Non‐enzymatic Disposable Electrochemical Sensor for Pyruvic Acid

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