Development of radioimmunoassay system for determination of human chorionic gonadotropin in human sera

Abstract

The main objective of this study was the preparation and evaluation of the primary reagents for the liquid phase human chorionic gonadotropin-radioimmunoassay (HCG-RIA) kit. Polyclonal antibody is specific and valid; monoclonal antibodies are more specific. In HCG-RIA technique, polyclonal antibodies for βHCG subunits with high binding and displacement % were used. 125I-HCG radioactive tracer was prepared with high yield 71.58 ± 0.92%, purity 99.2 ± 0.05%, and specific activity 170.42 ± 1.65 µCi/µg using chloramine T method, βHCG polyclonal antisera and HCG standards in assay buffer matrix were locally prepared. In-house HCG-RIA was developed with high sensitivity 0.5 mIU/ml, specificity (99%), precision (CV% < 6.4 and < 8.3 for intra-and inter-assay, respectively), and accuracy (recovery range 96.4–104.3%) could be used for quantitative estimation of HCG in human sera for monitoring pregnancy and diagnosis of gestational trophoblastic diseases.

Advances and Challenges in Nanomaterial-Based Electrochemical Immunosensors for Small Cell Lung Cancer Biomarker Neuron-Specific Enolase

“…Upon addition of another Ab (specific to Ag*), this results in the formation of a complex that precipitates out in the solution and is then analyzed for the concentration of target Ag by the amount of the Ag*–Ab complex. RIA is a highly sensitive, specific, and easy to operate technique with a low detection limit of even a few picograms of analyte …”

“…RIA is a highly sensitive, specific, and easy to operate technique with a low detection limit of even a few picograms of analyte. 70 1.3. Electrochemical Techniques.…”