Development of radioimmunoassays on microplate: Application for CA‐GI and CA‐Br tumor markers

Wu, James T.; Erali, Maria

doi:10.1002/jcla.1860070608

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…CA-BR (for CA 15-3) in-house microplate assay was used for the determination of serum CA 15-3 (24). Serum PSA was determined using Hybritech Tandem-E PSA kit and the serum CA 125 level was determined using the Abbot CA 125 EIA kit.…”

Section: Determination Of Serum Tumor Markersmentioning

confidence: 99%

Measurement of c‐erbb‐2 proteins in sera from patients with carcinomas and in breast tumor tissue cytosols: Correlation with serum tumor markers and membrane‐bound oncoprotein

Astill

Gagon

et al. 1995

Clinical Laboratory Analysis

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Using a commercial kit with antibodies against the ectodomain of c-erbB-2 protein, we detected c-erbB-2 immunoreactivity in human serum. We found that the percentages of patients with elevated serum c-erbB-2 immunoreactivities were 35, 21, and 9% in breast, prostate, and ovarian carcinoma, respectively. The majority of the elevated immunoreactivities were associated with sera containing highly elevated tumor markers with the highest in breast carcinoma (35%) and lowest in ovarian cancer (9%). Excellent correlations were also observed between the serum levels of c-erbB-2 immunoreactivity and the dominant tumor markers in serial specimens from individual cancer patients. We could also detect the c-erbB-2 immunoreactivity in the cytosols prepared from the breast tumor tissue for estrogen and progesterone receptor (ER & PgR) measurements using the same commercial kit for serum studies, and the intact c-erbB-2 oncoprotein (p185) in the extracts of the tissue membrane fractions with a different kit designed for tissue extract. The level of c-erbB-2 immunoreactivity in the cytosol from 124 human breast tumor specimens had an excellent correlation with the cell membrane concentrations of p185 (gamma = 0.89). Most of the elevated cytosol c-erbB-2 immunoreactivities were also found to associate with breast tumor specimens containing low concentrations of ER & PgR. It appears that measuring the c-erbB-2 immunoreactivity potentially could be used as a prognostic marker without performing tissue biopsies and also as a serum tumor marker for managing cancer patients.

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Section: Determination Of Serum Tumor Markersmentioning

confidence: 99%