Development of real-time PCR assay for genotyping SNP rs41255693 in cattle &lt;i&gt;SCD&lt;/i&gt; gene

Kovalchuk, Svetlana N.; Климов, Е. А.

doi:10.3934/agrfood.2019.5.14

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…The SCD gene in cattle have 17088 bp sequence length and consists of 6 exons and 5 introns on chromosome 26 (Ohsaki et al 2009). Several studies have reported that there are an association between stearoyl-CoA desaturase (SCD) gene diversity and fatty acid contexture in Wagyu cattle (Taniguchi et al 2004), Chinese Simmental cattle (Wu et al 2012), Holstein cross cattle (Kovalchuk et al 2019), pigs (Estany et al 2014), sheep (Esteves et al 2019) and chickens (Furqon et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

Karimah

Noor

Jakaria

2021

J. Indonesian Trop. Anim. Agric.

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The objectives of this research was to analyze the polymorphisms of SNP g.10428C>T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C>T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C>T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C>T have potential as marker-assisted selection candidates for Bali cattle.

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Section: Introductionmentioning

confidence: 99%

Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

Karimah

Noor

Jakaria

2021

J. Indonesian Trop. Anim. Agric.

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Genetic Polymorphisms of SCD1 gene and its relationship with some reproductive traits in Holstein cows in Iraq

Khaleel,

Abdullah,

Omear

2023

TJAS

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The research was conducted at the Khalis cows station in Al-Khalis district - Diyala Governorate and the central laboratory of the Faculty of Agriculture - University of Tikrit for the period from 13/12/2020 to 1/2/2022, a sample of 63 Holstein cows, with the aim of extracting the genetic material and determining the genetic polymorphism of the SCD1 gene and its relationship to reproductive traits in Holstein cows, studying the percentage of distribution of genetic structures in the cows that were studied, the allele frequency and calculating the value of chi-square (χ2) were determined. The genotypes frequencies for the SCD1 gene in the studied cow samples were 47.62, 46.03 and 6.35% for AA, AV and VV genotypes respectively. The value of the chi-square (χ2) was highly significant (P < 0.01). The frequency of allele A and V were 0.71 and 0.29 respectively. Some reproductive traits were significantly affected by the different genotypes of the SCD1 gene. Cows with the genotype AA achieved the highest Days Open (106.70 ± 5.84 days), while the genotypes VV and AV were 105.93 ± 14.25 and 92.82 ± 5.89 days respectively. The cows with AA genotype was the highest calving interval (389.77 ± 5.90 days), while the cows of the VV and AV genotypes reached 386.75 ± 14.39 and 374.27 ± 5.96 days respectively. It can be concluded by studying the genetic polymorphism of the SCD1 gene that it is possible to improve the reproductive traits in cows. We also recommend applying the study to a larger sample for several seasons and different locations of the gene, while studying more economic characteristics to give more accurate results for the application of the selection and exclusion strategy.

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Development of real-time PCR assay for genotyping SNP rs41255693 in cattle <i>SCD</i> gene

Cited by 2 publications

References 17 publications

Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

Identification of SNP g.10428C>T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

Genetic Polymorphisms of SCD1 gene and its relationship with some reproductive traits in Holstein cows in Iraq

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