Development of retrotransposon-based SSAP molecular marker system for study of genetic diversity in sea holly (Eryngium maritimum L.)

Abstract

Eryngium maritimum L. is a wild plant species threatened or endangered in most of Northern Europe, where species is on the northern margin of its distribution range. Recent studies have found reduction of size and even extinction of many populations. Assessment of genetic diversity in natural populations of endangered wild plant species can reflect condition and fitness of particular population and inform decisions on appropriate conservation measures. Application of inter simple sequence repeat markers and chloroplast DNA sequencing could not resolve genetic relationship between E. maritimum populations in Northern Europe. Therefore, the more sensitive retrotransposon-sequence-specific amplification polymorphism (SSAP) molecular marker system was developed. Six Ty1-copia long terminal repeat retrotransposons were isolated from E. maritimum genome (Tem1 -Tem6) and assessed for their utility as molecular markers in this species. Two retrotransposons -Tem2 and Tem5 -were recognized as most informative based on the level of polymorphism and SSAP banding pattern quality. On average, 20.4% of SSAP bands were polymorphic for the five most informative primer combinations in a set of 150 Northern European E. maritimum plants from 13 locations, providing a useful tool for assessment of genetic diversity in this endangered species.

Application of sequence specific amplified polymorphism (SSAP) and simple sequence repeat (SSR) markers for variability and molecular assisted selection (MAS) studies of the Mexican guava

“…S-SAP showed lower similarity values than AFLP, which indicates that S-SAP allows for detecting more intraspecific variability in guava than AFLP. The results obtained indicate that S-SAP is more informative for studies of variability and polymorphism of P. guajava, as has been reported for other crops of economic relevance such as barley (Soleimani et al, 2005;Shan et al, 2012), grapevine (Stajner et al, 2015), holly (Levina et al, 2010), and citrus (Biswas et al, 2011), among others.…”

Biological Flora of the British Isles: Eryngium maritimum

“…Taking the Baltic Coast from Poland to Finland, including the Swedish Islands Gotland and Öland, on the margin of the distribution range in Northern Europe the genetic diversity between and within populations is very low (Ievina et al . ).…”

“…On the 150 km coastline of the Gulf of Gda nsk, genetic diversity is very low and genetic distance is related to the direction of sea currents influenced by the western winds (Minasiewicz et al 2011). Taking the Baltic Coast from Poland to Finland, including the Swedish Islands Gotland and € Oland, on the margin of the distribution range in Northern Europe the genetic diversity between and within populations is very low (Ievina et al 2010).…”

The use of nondestructive methods to assess a physiological status and conservation perspectives of Eryngium maritimum L.

“…Various aspects of E. maritimum biology have been investigated more recently along coasts of the Baltic and the North sea including population trends in Lithuania (Olšauskas 1996;Olšauskas and Urbonienė 2008) and Poland (Łabuz 2007; Żółkoś et al 2007) as well as demography and germination biology in Norway (Curle et al 2007). Biogeography and genetic diversity of E. maritimum was studied recently using molecular markers (Clausing et al 2000;Kadereit and Westberg 2007;Ievina et al 2009Ievina et al , 2010.…”