Development of sandwich chemiluminescent immunoassay based on an anti-staphylococcal enterotoxin B Nanobody–Alkaline phosphatase fusion protein for detection of staphylococcal enterotoxin B

Sun, Tieqiang; Zhao, Zunquan; Li, Wentao; Xu, Zehua; He, Hongwei; Ning, Baoan; Jiang, Yongqiang; Gao, Zhixian

doi:10.1016/j.aca.2020.01.032

Cited by 38 publications

(24 citation statements)

References 45 publications

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“…The assay showed a low cross-reactivity to staphylococcal enterotoxin C (SEC) but none to staphylococcal enterotoxin A (SEA) or to SpA, avoiding the false positive problems described previously. The substitution of the capture nanobody by a mAb and of the detection phage-nanobody by an anti-SEB nanobody-AP fusion and chemiluminescent detection did not improve either the LOD (1.44 ng/mL) or the dynamic range of the assay (3.12-50 ng/mL), although the cross-reactivity in the presence of SEA was also negligible [104]. The production of singledomain antibody fragments has also been reported for the analysis of the biomarker OmpU a major protein of the membrane of V. parahaemolyticus, a pathogenic bacterium that can be present in raw and undercooked seafood, although the assays were not applied to food analysis [114].…”

Section: Foodborne Pathogens and Other Biotoxinsmentioning

confidence: 91%

Recombinant antibodies and their use for food immunoanalysis

et al. 2021

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Section: Foodborne Pathogens and Other Biotoxinsmentioning

confidence: 91%

Recombinant antibodies and their use for food immunoanalysis

et al. 2021

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“… Nouri et al (2018) designed a new kit for the detection of SEA in milk, with a detection time of about 15 min and a sensitivity of 15.6 ng of toxin. For instance, Sun et al (2020) developed a sandwich chemiluminescent immunoassay (CLIA) to detect SEB, an anti-SEB monoclonal antibody was used as the capture antibody, and Nb37-ALP was used as the detector antibody; the detection limit was 1.44 ng/ml. In another sandwich ELISA assay, nano-antibodies acted as capturing antibodies, and the detection antibodies were acted by phage nanoantibodies with amplified signal properties.…”

Section: Available Methods For Detecting S Aureusmentioning

confidence: 99%

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang

Yang

et al. 2021

Front. Microbiol.

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Staphylococcus aureus (S. aureus) is the top ordinary pathogen causing epidemic and food poisoning. The authentication of S. aureus has great significance for pathologic diagnosis and food hygiene supervision. Various biosensor methods have been established for identification. This paper reviews the research progress of aptasensors for S. aureus detection, focusing on the classification of aptamer technologies, including optical aptasensors and electrochemical aptasensors. Furthermore, the feasibility and future challenges of S. aureus detection for aptamer assays are discussed. Combining aptasensors with nanomaterials appears to be the developing trend in aptasensors.

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“…In contrast to the therapeutic use, phage display was applied to generate nanobodies against SEB from camel immune and naive libraries useful as a diagnostic tool. These nanobodies were either directly coupled with alkaline phosphatase in a sandwich-ELISA ( Sun T. et al., 2020 ), or used for western-blot and ELISA in an indirect detection system ( Zanganeh et al., 2019 ).…”

Section: Antibodies Against Toxinsmentioning

confidence: 99%

Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy

Roth

Wenzel

Ruschig

et al. 2021

Front. Cell. Infect. Microbiol.

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Antibodies are essential molecules for diagnosis and treatment of diseases caused by pathogens and their toxins. Antibodies were integrated in our medical repertoire against infectious diseases more than hundred years ago by using animal sera to treat tetanus and diphtheria. In these days, most developed therapeutic antibodies target cancer or autoimmune diseases. The COVID-19 pandemic was a reminder about the importance of antibodies for therapy against infectious diseases. While monoclonal antibodies could be generated by hybridoma technology since the 70ies of the former century, nowadays antibody phage display, among other display technologies, is robustly established to discover new human monoclonal antibodies. Phage display is an in vitro technology which confers the potential for generating antibodies from universal libraries against any conceivable molecule of sufficient size and omits the limitations of the immune systems. If convalescent patients or immunized/infected animals are available, it is possible to construct immune phage display libraries to select in vivo affinity-matured antibodies. A further advantage is the availability of the DNA sequence encoding the phage displayed antibody fragment, which is packaged in the phage particles. Therefore, the selected antibody fragments can be rapidly further engineered in any needed antibody format according to the requirements of the final application. In this review, we present an overview of phage display derived recombinant antibodies against bacterial, viral and eukaryotic pathogens, as well as microbial toxins, intended for diagnostic and therapeutic applications.

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Development of sandwich chemiluminescent immunoassay based on an anti-staphylococcal enterotoxin B Nanobody–Alkaline phosphatase fusion protein for detection of staphylococcal enterotoxin B

Cited by 38 publications

References 45 publications

Recombinant antibodies and their use for food immunoanalysis

Recombinant antibodies and their use for food immunoanalysis

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy

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