Development of Sandwich Double-Competitive ELISA for Sulfonamides. Comparative Analytical Characteristics and Matrix Effect Resistance

Burkin, Maksim A.; Nuriev, R. I.; Wang, Zhanhui; Galvidis, Inna A.

doi:10.1007/s12161-017-1036-6

Cited by 18 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar matrix effect was also observed in the immunoassay for other hazards in vegetables and fruits, 12,13 but the exact source of the interference remained unclear. In fish, meat and other food stuffs of animal origin, some proteins in matrix have been verified the main source of the interference investigated.…”

Section: Introductionmentioning

confidence: 55%

“…Such false‐positive results indicated clearly the serious interference of the matrix to the immunoassay. Some previous studies also reported similar matrix effects in the immunoassay of vegetables or other herb‐origin samples, but few information was indicated about the exact source of the interference 10‐13,22 . Some proteins have been verified to induce significant matrix effect in the immunoassay of fish, meat and other animal‐origin food stuffs 23‐25 .…”

Section: Resultsmentioning

confidence: 96%

“…Some previous studies also reported similar matrix effects in the immunoassay of vegetables or other herb-origin samples, but few information was indicated about the exact source of the interference. [10][11][12][13]22 Some proteins have been verified to induce significant matrix effect in the immunoassay of fish, meat and other animal-origin food stuffs. [23][24][25] But the protein concentration here in the vegetables crude extract was calculated as 0.016-0.051 mg mL −1 (Fig.…”

Section: Matrix Effect Analysismentioning

confidence: 99%

See 2 more Smart Citations

The effect of chlorophyll on the enzyme‐linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference

et al. 2021

View full text Add to dashboard Cite

BACKGROUND There is now an increasing demand for the immunoassay of procymidone residue in foodstuffs. However, the matrix interference could significantly affect the analysis. Till now there is no detailed information on the source of the interference and the mechanism involved, which greatly limits the real application of these techniques. RESULTS Significant matrix effect was observed in the enzyme‐linked immunosorbent assay (ELISA) of procymidone in negative vegetable samples (leek, broccoli and cucumber). By the investigation with both vegetable extracts and standard solutions, the chlorophyll was confirmed as an important source of the matrix effect. Therefore, a new strategy was proposed for the pretreatment based on the exploitation of 5‐sulfosalicylic acid. It was demonstrated to effectively eliminate chlorophyll and exhibited little effect on procymidone and the competitive indirect ELISA (ci‐ELISA) performance. The established technique was validated with different vegetables. With the spiking concentration of procymidone investigated, the recovery rate of ci‐ELISA was 71.52–120.37%, and the relative standard deviation was 4.05–17.61%. CONCLUSION Chlorophyll was for the first time illuminated as an important source of matrix interference to the immunoassay of procymidone in vegetables. A new pretreatment based on 5‐sulfosalicylic acid was established to remove chlorophyll and therefore eliminate the matrix effect. Validated with different vegetable samples, the new technique was demonstrated much better efficiency in comparison to conventional methods, which indicated its promising application for the development of immunoassays of herb‐origin samples. © 2021 Society of Chemical Industry.

show abstract

Section: Introductionmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 96%

Section: Matrix Effect Analysismentioning

confidence: 99%

See 1 more Smart Citation

The effect of chlorophyll on the enzyme‐linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In order to adapt the ELISA system to the measurement of COL in foodstuff, dairy products and chicken eggs, it was necessary to evaluate the effect of sample matrix on the immunochemical interaction "antibody-antigen" and develop a procedure of sample preparation to eliminate possible interferences. As we have repeatedly seen, the matrix effect of milk consists in some suppression of the binding of antibodies to antigen (Burkin, Nuriev, Wang, & Galvidis, 2018;Galvidis & Burkin, 2010). It can be seen from Figure 4 that when diluting, the milk matrix effect weakened, and the optical signal of reaction was restored.…”

Section: Examination Of Matrix Effectmentioning

confidence: 64%

Development of indirect competitive enzyme-linked immunoassay of colistin for milk and egg analysis

Galvidis

Eremin

Burkin

2020

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

Polyclonal antibodies were obtained by immunizing rabbits with polymyxin B (PMB) conjugated to bovine serum albumin. Based on the heterologous coating conjugate of PMB with periodateoxidized gelatin and the resulting antibodies, an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed to detect residual colistin (COL), a structural analogue of PMB in livestock products. The developed analysis was characterized by close specificity towards PMB (100%) and COL (88%), sensitivity (IC 50 = 5.7 ng/mL), dynamic range (IC 20 -IC 80 = 1.0-80 ng/mL) and the detection limit of COL (IC 10 = 0.4 ng/mL). It was possible to quantify the antibiotic in milk and eggs without special and labour-intensive sample pretreatment using a simple 10-and 50-fold dilution of the corresponding samples. The developed analysis system made it possible to record the maximum residue limit of COL in dairy products (50 μg/kg) and eggs (300 μg/kg) with 89-104% recovery of analyte.

show abstract

“…In the literature there are studies on the development of ELISA methods for the determination of antibiotics (Burkin et al, 2018), as well as studies on their application on real samples (Shen and Jiang, 2005;Mahmoudi et al, 2014), but there are no data on similar evaluations. Certainly, the European requirements for screening methods are stricter (EC, 2002), and these methods should be examined in order to determine detection capability, as well as check them by using reference materials based on honey matrices and participation in proficiency tests.…”

Section: Rsd-relative Standard Deviation N-number Of Measurementsmentioning

confidence: 99%

Evaluation of ELISA tests as screening methods for determination of antibiotics and sulfonamides in honey

Jaksic¹,

Mihaljev²,

Kartalović³

et al. 2018

Food & Feed Res

View full text Add to dashboard Cite

The health safety of honey includes the correctness in terms of the presence of different contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in food. In this paper the application of ELISA tests for determination of selected antibiotics and sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was evaluated after application on honey samples spiked with standard solution of a particular analyte. Samples were prepared according to the instructions of the ELISA test manufacturer referring to honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory accuracy (73-111%) and precision (14-16%). Recovery for sulfametoxypyridazin was low (40%), and for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in relation to the requirements of the legislation (0.075-3 µg/kg). The test kits can be used to screen the presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the obtained validation parameters.

show abstract

Development of Sandwich Double-Competitive ELISA for Sulfonamides. Comparative Analytical Characteristics and Matrix Effect Resistance

Cited by 18 publications

References 26 publications

The effect of chlorophyll on the enzyme‐linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference

The effect of chlorophyll on the enzyme‐linked immunosorbent assay (ELISA) of procymidone in vegetables and the way to overcome the matrix interference

Development of indirect competitive enzyme-linked immunoassay of colistin for milk and egg analysis

Evaluation of ELISA tests as screening methods for determination of antibiotics and sulfonamides in honey

Contact Info

Product

Resources

About