Development of SCAR Marker for Discrimination of Artemisia princeps and A. argyi from Other Artemisia Herbs

Lee, Mi Young; Doh, Eui Jeong; Park, Chae Haeng; Kim, Young Hwa; Kim, Eung Soo; Ko, Byong Seob; Oh, Seung Eun

doi:10.1248/bpb.29.629

Cited by 59 publications

(37 citation statements)

References 9 publications

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“…Similarly, Wang et al 18) believed that trnL-F sequences are highly homogeneous in Artemisia L. and have limitations in phylogeny. Lee et al 19) amplified 254 bp sequence-characterized amplified regions using Fb and R7 primer sets to distinguish A. argyi from four other Korean Artemisia species; however, A. argyi could not be distinguished from A. princeps. Liu et al 20) reported that ITS2, unlike rbcL, matK, and psbA-trnH, could be used as a potential DNA barcode to correctly identify the medicinal plants in Artemisia L. Gao et al 21) evaluated the ability of five DNA regions (rbcL, matK, ITS, ITS2, and psbAtrnH) to identify 3490 Asteraceae sequences that represent 2315 species belonging to 494 genera.…”

Section: -11)mentioning

confidence: 99%

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

Quanxi

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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Folium Artemisiae Argyi is an important herb in traditional Chinese medicine. It is commonly used in moxibustion, medicine, etc. However, identifying Artemisia argyi is difficult because this herb exhibits similar morphological characteristics to closely related species and counterfeits. To verify the applicability of DNA barcoding, ITS2 and psbA-trnH were used to identify A. argyi from 15 closely related species and counterfeits. Results indicated that total DNA was easily extracted from all the samples and that both ITS2 and psbA-trnH fragments can be easily amplified. ITS2 was a more ideal barcode than psbA-trnH and ITS2 psbA-trnH to identify A. argyi from closely related species and counterfeits on the basis of sequence character, genetic distance, and tree methods. The sequence length was 225 bp for the 56 ITS2 sequences of A. argyi, and no variable site was detected. For the ITS2 sequences, A. capillaris, A. anomala, A. annua, A. igniaria, A. maximowicziana, A. princeps, Dendranthema vestitum, and D. indicum had single nucleotide polymorphisms (SNPs). The intraspecific Kimura 2-Parameter distance was zero, which is lower than the minimum interspecific distance (0.005). A. argyi, the closely related species, and counterfeits, except for Artemisia maximowicziana and Artemisia sieversiana, were separated into pairs of divergent clusters by using the neighbor joining, maximum parsimony, and maximum likelihood tree methods. Thus, the ITS2 sequence was an ideal barcode to identify A. argyi from closely related species and counterfeits to ensure the safe use of this plant. Key words Folium Artemisiae Argyi; DNA barcoding; ITS2; psbA-trnHFolium Artemisiae Argyi, the dried leaves of Artemisia argyi Levl. et Van, is an important herb in traditional Chinese medicine and is commonly used in moxibustion, medicine, food, or medicinal bath.1-4) A. argyi is widely distributed in Korea, Mongolia, Japan, and the Russia Far East.1,5,6) Folium Artemisiae Argyi was first recorded in "Shi Jing" near 1100 BC and in many clinical and medicine literature, such as "Wu Shi Er Bing Fang," "Ben Cao Gang Mu," and "Jin Gui Yao Lve." 3,7) In traditional Chinese medicine, Folium Artemisiae Argyi was considered to have bitter, pungent, and warm properties and associated with the liver, spleen, and kidney meridians.8) Folium Artemisiae Argyi prevents and cures gynecological, respiratory, and dermatological diseases, such as menstruation-related symptoms, infertility, dysmenorrhea, inflammation, hemostasis, tuberculosis, asthma, and eczema. 9-11)Folium Artemisiae Argyi contains complex ingredients, including essential oil, polysaccharide, flavonoid, tannin, sterols, and triterpenes. [12][13][14][15][16] Unfortunately, identifying A. argyi is difficult because this plant exhibits similar morphological characteristics to closely related species and counterfeits, including congeneric plants Artemisia lavandulifolia, Artemisia anomala, Artemisia princeps, Artemisia indica, Artemisia stolonifera, Dendranthema vestitum, and Dendranthema indicum.1-...

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Section: -11)mentioning

confidence: 99%

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

Quanxi

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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“…For the development of SCAR marker, we used five accessions of the plant as it is the high altitude plant and has very low availability. However, many published studies showed the development of RAPD based marker from the large number of accessions but these plants have good availability such as genus Artemisia [21], Panax species [22], Phyllanthus species [23], Ganoderma lucidum [24], Ipomoea mauritiana [25], Bemisiatabaci (Genn.) [26].…”

Section: Discussionmentioning

confidence: 99%

Scar Marker Development for the Correct Identification of Iris Ensata

Mughees

Jha

Ahmad

et al. 2017

Int J Pharm Pharm Sci

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Objective: The objective of this research was to develop the RAPD based SCAR marker for the correct identification of the Iris ensata Thunb. (I. ensata) plant from its adulterants.Methods: Five samples of I. ensata. from the different geographical area were used in this study. The plant genomic DNA was isolated with the CTAB method with some modification (as dried samples were also used). After that, polymorphism was checked with the help of the 10-mer random primers of OPAA and BG series. Then, the bands of interest were eluted and cloned into pGEMT easy vector for the sequencing. Finally, the sequence is used to develop the SCAR primers (Ir-f andIr-R) specific for I. ensata and the developed primers also validated with respect to the market samples.Results: A putative 580 bp sequence specific for Iris ensata was identified from the randomly amplified polymorphic DNA (RAPD) analysis. To overcome the main limitation of RAPD it has been converted into SCAR markers. So that, this specific band was then eluted, cloned and sequenced. After that, SCAR primers (Ir-F and Ir-R) were synthesized by using this sequence. For the validation of the synthesized SCAR primers, they were tested with respect to the market samples. The amplicon of 260 bp was produced by the SCAR primers in the authentic I. ensata but market samples did not produce any bands with the synthesized SCAR primers. Conclusion:The results of this study show a high level of polymorphism in the RAPD pattern of the different accessions of the plant. Furthermore, this study results in the successful development of the RAPD based SCAR marker for the identification of the I. ensata.

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“…유럽이 원산지인 큰느타리버섯은 주로 유럽 남부, 중앙 아시아, 북아프리카, 지중해 연안 등 아열대 지방의 건조 성 초원지대에 자생하고 있으며 (Zadrazil, 1974 (Panner et al, 1993). 이러한 RAPD의 단점을 보완하기 위한 방법으로 RAPD 밴드의 염기서열을 기반으로 하는 SCAR(Sequence characterized amplified region) 마커 개 발에 관한 연구가 활발히 이루어지고 있다 (Panner et al, 1993;Song et al, 1996;Bang et al, 2004;Koveza and Gostimsky, 2005;Lee et al, 2006;Qin et al, 2006 …”

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