Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs

Yamakawa, Nao; Sato, Chihiro; Miyata, Shinji; Maehashi, Eri; Toriyama, Masaru; Sato, Noriko; Furuhata, Kimio; Kitajima, Ken

doi:10.1016/j.biochi.2007.04.010

Cited by 25 publications

(17 citation statements)

References 41 publications

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“…A hybridoma cell 4F7 that produces monoclonal IgG specific for α2,9-linked polysialic acid (polySia) structure (Miyata et al, 2006) and a hybridoma cell 3G9 that produces monoclonal IgM specific for 8-O-sulfated sialic acid residue (Ohta et al, 1999;Yamakawa et al, 2007) were established and grown in serum-free medium (CosMedium001, Cosmobio, Tokyo, Japan). Monoclonal antibodies 4F7 and 3G9 were purified from culture supernatants by precipitation with 50% saturated ammonium sulfate, followed by gel filtration chromatography on Sephacryl S-300 (GE Healthcare).…”

Section: Antibodies Against Flagellasialinmentioning

confidence: 99%

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Kambara

Shiba

Yoshida

et al. 2011

Cell Struct. Funct.

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ABSTRACT. Flagellar movement of the sea urchin sperm is regulated by intracellular Ca 2+ . Flagellasialin, a polysialic acid-containing glycoprotein, as well as other membrane proteins seems responsible for the Ca 2+ control. To elucidate the mechanism of Ca 2+ dynamics underlying flagellar movement, we analysed the sperm's mechanosensory behavioural responses by using microtechniques. In sea water containing 10 mM Ca 2+ , the sperm swim in circular paths. When a mechanical stimulus was applied to the sperm head with a glass microstylus, the sperm showed a series of flagellar responses, consisting of a stoppage of beating (quiescence) and a recovery of swimming in a straight path, followed by swimming in a circular path again; as the result the sperm avoided the obstacle. Ca 2+ -imaging with Fluo-4 showed that the intracellular Ca 2+ was high in the quiescence and gradually decreased after that. The effects of blockers and antibodies against candidate components revealed that the Ca 2+ influx was induced by Ca 2+ channels and the Ca 2+ efflux was induced by a flagellasialin-related Ca 2+ -efflux system, plasma membrane Ca 2+ -ATPases and the K + -dependent Na + /Ca 2+ exchanger. The results show that the Ca 2+ -dependent mechanosensory behaviour of the sea urchin sperm is regulated by organized functioning of the membrane environment including the plasma membrane proteins and flagellasialin.

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Section: Antibodies Against Flagellasialinmentioning

confidence: 99%

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Kambara

Shiba

Yoshida

et al. 2011

Cell Struct. Funct.

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“…4MU-Neu5Gc and 4MU-Kdn were from Dr. Kimio Furuhata (70). 25 nmol of 4MU-Sia was digested in 90 l of 50 mM sodium acetate, pH 4.75 (NEU1), or PBS, pH 6.5 (AUS).…”

Section: Enzyme Digests Of 4-methylumbelliferyl Sialic Acidsmentioning

confidence: 99%

Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups

Davies

Pearce

Tessier

et al. 2012

Journal of Biological Chemistry

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Background:The sialic acid N-glycolylneuraminic acid (Neu5Gc) shows conserved suppression of expression in vertebrate brains, suggesting brain-specific toxicity. Results: ␣2-8-Linked Neu5Gc incorporated into the neural glycan polysialic acid (polySia) resists sialidase breakdown through conformational effects. Conclusion: Neu5Gc in brain would prevent rapid turnover of surface polySia. Significance: This mechanism potentially underlies the evolutionary suppression of Neu5Gc expression in vertebrate brains.

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“…No significant degradation of Neu5Ac, Neu5Gc, and Kdn has been reported. For SiaS, the glycosidic bonds of SiaS were completely hydrolyzed under the hydrolysis conditions, while their sulfate esters are hydrolyzed by, at most, 4% [64]. No apparent desulfation occurred under the labeling conditions.…”

Section: Fluorometric Hplc Analysismentioning

confidence: 96%

Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis

Kitajima

Varki

Sato

2013

Topics in Current Chemistry

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Although the structural diversity of sialic acid (Sia) is rapidly expanding, understanding of its biological significance has lagged behind. Advanced technologies to detect and probe diverse structures of Sia are absolutely necessary not only to understand further biological significance but also to pursue medicinal and industrial applications. Here we describe analytical methods for detection of Sia that have recently been developed or improved, with a special focus on 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac), N-glycolylneuraminic acid (Neu5Gc), deaminoneuraminic acid (Kdn), O-sulfated Sia (SiaS), and di-, oligo-, and polysialic acid (diSia/oligoSia/polySia) in glycoproteins and glycolipids. Much more attention has been paid to these Sia and sialoglycoconjugates during the last decade, in terms of regulation of the immune system, neural development and function, tumorigenesis, and aging.

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Development of sensitive chemical and immunochemical methods for detecting sulfated sialic acids and their application to glycoconjugates from sea urchin sperm and eggs

Cited by 25 publications

References 41 publications

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Mechanism Regulating Ca2+-dependent Mechanosensory Behaviour in Sea Urchin Spermatozoa

Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups

Advanced Technologies in Sialic Acid and Sialoglycoconjugate Analysis

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