Development of sensitive direct and indirect enzyme-linked immunosorbent assays (ELISAs) for monitoring bisphenol-A in canned foods and beverages

Abstract

Enzyme-linked immunosorbent assays (ELISAs) are investigated in this work for the detection of bisphenol-A (BPA), a plastic monomer and a critical contaminant in food and environment. A series of polyclonal antibodies generated in vivo using BPA-butyrate-protein conjugate and BPA-valerate-protein conjugate were evaluated on direct and indirect competitive assay formats with five competing haptens (BPA-butyrate, BPA-valerate, BPA-crotonate, BPA-acetate, and BPA-2-valerate). Two indirect ELISAs and one direct ELISA exhibiting high sensitivity and specificity for BPA were developed. The 50 % inhibition of antibody binding (IC(50)) values were 0.78 ± 0.01-1.20 ± 0.26 μg L(-1), and the limits of detection as measured by the IC(20) values were 0.10 ± 0.03-0.20 ± 0.04 μg L(-1). The assays were highly specific to BPA, only displaying low cross-reactivity (3-8 % for the indirect assays and 26 % for the direct assay) for 4-cumylphenol (4-CP), at pH 7.2. The degree of cross-reaction of 4-CP was influenced by the antibody/hapten conjugate combination, assay conditions, and the assay format. The assays were optimized for the analysis of BPA in canned vegetables, bottled water and carbonated drinks. The limits of quantification for these three evaluated sample types, based on the spike and recovery data, were 0.5, 2.5, and 100 μg L(-1), respectively.

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

“…However, the intensive use of herbicides has resulted in serious pollution of agricultural products, soil resources, especially water resources, which pollute the whole ecological system, and possibly leads to a serious threat to the safety of the water resources (Brender & Weyer, 2016;Cruzeiro, Rocha, Pardal, & Rocha, 2016;Lei et al, 2011). In recent years, more and more attention has been paid to herbicide residue in water samples worldwide (Feng, Yu, Liu, Liu, & Sun, 2015;Lu, Peterson, Gooding, & Lee, 2012;Oberleitner et al, 2014;Wu et al, 2013;Zhang et al, 2011). These residual herbicides in water samples also presented a high risk to human health (Barbosa, Solano Mde, & Umbuzeiro Gde, 2015;Benito-Peña, MorenoBondi, Orellana, Maquieira, & van Amerongen, 2005;Kolosova et al, 2017;Sekhotha, Monyeki, & Sibuyi, 2016;Zhang et al, 2011).…”

A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4′-(aminomethyl)fluorescein

“…3,10,11 Recently, molecular imprinting of detection has become more popular. ELISA 19 and CLEIA 1 are heterogeneous methods which need 2-3 h and several times of washing, while FPIA is a homogeneous method which needs no separation or washing steps but just a short time of 10 min. Immunoassays have been widely studied and used for the detection of BPA, and many research reports are about antibody production and development of immunoassay methods and established ic-ELISA, 12 ELISA, 13,14 CLEIA, 15 FLISA, 16 and FPIA 17 methods which were used for determination based on a polyclonal antibody, monoclonal antibody 18 or single antibody.…”

Determination of metolcarb residues by a biotin–streptavidin-amplified enzyme-linked immunosorbent assay in vegetables and edible fungus

“…These effects must be removed for assay validation. Dilution is widely used to reduce this interference; however, this also reduces the sensitivity of the assay (Lu, Peterson, Gooding, & Lee, 2012;Wang, Zhang, Yang, Wang, & Zhang, 2005). 3.4.1.…”