Development of solid phase radioimmunoassay system using new polymeric magnetic micro-spheres

Sallam, Kh. M.; Sheha, Reda R.; El‐Zahhar, Adel A.

doi:10.1007/s10967-011-1188-6

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Finally, microbeads can be manoeuvred with high levels of spatial-and temporal resolution under electrical [5,8], magnetic [7,11] and/or fluidic control [5,12]. Such flexibility facilitates highly sensitive detection with optimized parameters [15] and multiplexing [16].…”

Section: Introductionmentioning

confidence: 99%

“…However, the antibody of interest is biotinylated (biotin attached to the antibody), involving very time-consuming procedures such as multi-step reactions, washings and centrifugations. Alginate, a natural, biocompatible and highly stable carbohydrate-based hydrogel, has been widely used as a drug carrier and encapsulation material [15] because it can be polymerized by simple gelation with divalent cations such as calcium ion [16]. Alginate-based immobilization methods in both film and sphere configurations have been reported with encouraging results [14,18,19], but the immobilization procedure is still rather complicated, involving multiple polymerization or conjugation steps for both hydrogels and antibodies, and multiple rounds of incubation and separation steps.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic one-step synthesis of alginate microspheres immobilized with antibodies

Chen

Kim

Zhang

et al. 2013

J. R. Soc. Interface.

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Micrometre-and submicrometre-size functionalized beads are frequently used to capture targets of interest from a biological sample for biological characterizations and disease diagnosis. The main challenge of the microbead-based assay is in the immobilization of probe molecules onto the microbead surfaces. In this paper, we report a versatile droplet microfluidics method to fabricate alginate microspheres while simultaneously immobilizing anti-Mycobacterium tuberculosis complex IgY and anti-Escherichia coli IgG antibodies primarily on the porous alginate carriers for specific binding and binding affinity tests. The binding affinity of antibodies is directly measured by fluorescence intensity of stained target bacteria on the microspheres. We demonstrate that the functionalized alginate microspheres yield specificity comparable with an enzyme-linked immunosorbent assay. The high surface area-to-volume ratio of the functionalized porous alginate microspheres improves the detection limit. By using the droplet microfluidics, we can easily modify the size and shape of alginate microspheres, and increase the concentration of functionalized alginate microspheres to further enhance binding kinetics and enable multiplexing.

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