Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation

Rahman, Mohammed Ataur; Ali, Md. Rostom; Sarder, Md. Rafiqul Islam; Mollah, M.F.A.; Khan, Najmus Sakib

doi:10.1016/j.cryobiol.2016.10.004

Cited by 20 publications

(4 citation statements)

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“…Additionally, the overall genetic diversity of M. armatus populations in this study was over 0.5, showing a relative high diversity (Grant and Bowen, 1998). In general, combing our data and literature data, the genetic diversity of M. armatus maintains at a relatively high level, although the size of natural population is declining as reported (Hossain et al, 2012;Rahman et al, 2016;Xue, 2018). Furthermore, genetic differentiation between most populations is pronounced, regardless of spatial scale.…”

Section: Discussionsupporting

confidence: 76%

“…However, due to overfishing and habitat loss, the wild population size of M. armatus gradually declines year by year (Hossain et al, 2012;Rahman et al, 2016;Xue, 2018). M. armatus is designated as an endangered species in Bangladesh (IUCN Bangladesh, 2000) and has been classified as least concern by the International Union for Conservation of Nature (IUCN) (IUCN, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Yang

Wang²,

Wu³

et al. 2023

Front. Mar. Sci.

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IntroductionMastacembelus armatus is a commercially valuable fish, normally distributed in southern China and Southeast Asia. The natural population size of M. armatus is shrinking in recent years because of overfishing and habitat loss. In order to clarify the genetic diversity and differentiation of M. armatus populations, we collected 114 samples from eight populations in southern China and Vietnam and analyzed their population structure using nuclear ribosomal DNA sequences, the concatenated 18S and ITS2 regions.MethodsGenomic DNA from the fin clip was extracted and sequenced on an Illumina novaseq 6000 (Illumina, USA) high-throughput sequencing platform in accordance with the manufacturer’s instructions. After assembly and annotation, haplotype diversity, TCS network analysis, AMOVA analysis, population pairwise genetic distances, and UPGMA tree construction were conducted based on the concatenated sequences of 18S and ITS2.Results and discussionIn total, eleven nrDNA haplotypes were detected based on the concatenated sequences of 18S and ITS2. Amongst, three haplotypes were the main haplotypes, as representatives of three corresponding Clusters. There were two major Clusters in China, however, the Cluster in Vietnam was significantly divergent from the other two in China, likely due to the lack of river connection between China and Vietnam. Interestingly, based on low FST value, we found that gene flow occurred between the isolated island, Hainan Province, and the mainland China of Guangxi Province, probably as a result of exposed continental shelf connected them during glacial periods. In general, combing our data and literature data, genetic diversity and differentiation of M. armatus populations are relatively high regardless of spatial scale, although their natural population size is declining. This suggests that it is not too late to adopt measures to protect M. armatus, which benefits not only species itself but also the whole ecosystem.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Yang

Wang²,

Wu³

et al. 2023

Front. Mar. Sci.

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“…The consequences of cryopreservation on larval survival was not also significant for a variety of fishes. 50,87,94,95 In conclusion, although cryopreservation reduced gamete and progeny quality, negative effects can be minimized by choosing METH-5% cryoprotectant for Levantine scraper germplasm cryobanking. Results presented here validate the use of blastomere cleavage patterns or embryonic viability during organogenesis as a quick and easy tool for predicting the quality of embryo batches using frozen-thawed sperm.…”

Section: Discussionmentioning

confidence: 94%

“…In addition, growth rate of larvae (measured as TL) from the CPA treatments were similar to the control from hatch until 15 dph. The consequences of cryopreservation on larval survival was not also significant for a variety of fishes …”

Section: Discussionmentioning

confidence: 99%

Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Zadmajid

Falahipour

Ghaderi

et al. 2019

Developmental Dynamics

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Background Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are sired with fresh and frozen‐thawed sperm. Results Cryopreserved sperm did not affect embryogenesis at two‐ and four‐cell stages, but impaired embryonic development at eight‐cell stage. Embryonic viability decreased at organogenesis, where only 34‐49% of embryos showed viability with frozen‐thawed sperm. Hatching success and percentage of normal hatched embryos declined when fertilized with frozen‐thawed sperm. Considering only frozen‐thawed cells the dimethyl sulfoxide (DMSO)‐5%, methanol (METH)‐5%, and METH‐10% treatments yielded highest hatch, while METH‐5% and propylene glycol‐5% yielded the most normal hatched embryos. Larval spinal torsion was higher for fresh than frozen‐thawed sperm, where larvae with spinal torsion showed vertebral fusion and shape alterations during exogenous feeding. Both fresh and cryopreserved treatments showed abnormalities in caudal skeleton, while rates of defective yolk‐sacs were higher for cryopreserved sperm, where larvae with defective yolks showed oversized yolk extension. Percentage of larvae with defective heads/eyes were also higher for cryopreserved sperm. Conclusions Results show how frozen‐thawed sperm impairs embryonic/larvae development and identifies frequency and position of abnormalities. Future studies should investigate how sperm DNA damage may have caused these alterations.

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Potential of Fish Gamete Cryopreservation in Conservation Programs in Bangladesh

Sarder

2020

Cryopreservation of Fish Gametes

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Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation

Cited by 20 publications

References 50 publications

Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Population genetics of zig-zag eel (Mastacembelus armatus) uncover gene flow between an isolated island and the mainland China

Outcomes of in vitro fertilization with frozen‐thawed sperm: An analysis of post‐thaw recovery of sperm, embryogenesis, offspring morphology, and skeletogenesis for a cyprinid fish

Potential of Fish Gamete Cryopreservation in Conservation Programs in Bangladesh

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