Development of Sphaerotheca fusca on susceptible, resistant, and temperature-sensitive resistant melon cultivars

Pérez-Garcı́a, Alejandro; Olalla, Lucı́a; Rivera, Eugenia; Pino, Daniel Del; Canovas, I.; Vicente, Antonio de; Torés, J. A.

doi:10.1016/s0953-7562(08)61992-9

Cited by 31 publications

(29 citation statements)

References 20 publications

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“…on sweet pea (Pisum sativum L.) (Poulter et al 2003;Viljanen-Rollinson et al 1998), Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci on melon (Cucumis melo L.) (Cohen and Eyal 1988;Floris and Alvarez 1996;Kuzuya et al 2003;Pérez-Garcia et al 2001), and Erysiphe polygoni DC. on mungbean (Vigna radiata (L.) Wilczek) (Reddy et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Development ofErysiphe pulchra, the causal agent of powdery mildew, on leaf disks of susceptible and resistant flowering dogwood (Cornus florida)

Windham

Trigiano

et al. 2006

Canadian Journal of Plant Pathology

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Understanding the relative contribution of the different resistance components is necessary to develop selection schemes and accelerate resistant-cultivar development. This study was conducted to investigate spore germination, infectionstructure formation, and fungal development of Erysiphe pulchra, the causal agent of powdery mildew, on leaf disks of six cultivars or lines of flowering dogwood (Cornus florida) with different levels of resistance. The cultivars and lines tested were grouped into the following three resistance categories: highly susceptible ('Cherokee Daybreak' and MW 94-60), moderately susceptible ('Cherokee Princess , and resistant ('Cherokee Brave' and 'Karen's Appalachian Blush'). Percentages of spore germination and secondary-appressoria formation were not significantly different among the cultivars and lines. Significantly less percent germinated conidia with branched hyphae were observed on resistant cultivars than on the moderately susceptible cultivar or line, which was less than on the highly susceptible cultivar or line. Infection efficiencies were significantly different among cultivars and lines in the three resistance categories, except that there were no differences between 'Cherokee Princess' and the resistant cultivars. Resistant cultivars supported shorter latent periods than moderately and highly susceptible cultivars or lines, but no differences in latent period were detected between the later two resistance categories. The recently released 'Karen's Appalachian Blush' expressed higher levels of resistance to powdery mildew than did 'Cherokee Brave', as indicated by the longer latent period and reduced relative sporulation of the pathogen. Résumé : Comprendre la contribution relative des différents éléments de la résistance est nécessaire pour établir des plans de sélection et accélérer le développement de cultivars résistants. La présente recherche fut menée pour étudier la germination des spores, la formation des structures d'infection et le développement fongique de l'Erysiphe pulchra, agent responsable de l'oïdium, sur des rondelles de feuilles de six cultivars ou lignées de cornouiller (Cornus florida) en fleurs possédant différents degrés de résistance. Les cultivars et lignées testés étaient regroupés selon trois catégories de résistance : très sensibles ('Cherokee Daybreak' et MW 94-60), moyennement sensibles ('Cherokee Princess' et MW 95-25) et résistants ('Cherokee Brave' et 'Karen's Appalachian Blush'). Les pourcentages de germination des spores et la formation d'appressoria secondaires n'étaient pas significativement différents parmi les cultivars et lignées. Le pourcentage de conidies germées avec hyphes ramifiées observé chez les cultivars résistants fut significativement moins élevé que ceux du cultivar et de la lignée moyennement sensibles, eux-même moins élevés que ceux du cultivar et de la lignée très sensibles. L'efficacité de l'infection fut significativement différente parmi les cultivars et lignées dans les trois catégories de résistance, sauf entre 'Chero...

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Section: Discussionmentioning

confidence: 99%

Development ofErysiphe pulchra, the causal agent of powdery mildew, on leaf disks of susceptible and resistant flowering dogwood (Cornus florida)

Windham

Trigiano

et al. 2006

Canadian Journal of Plant Pathology

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“…The isolate SF48 of Podosphaera fusca race 1 was routinely grown in vitro on cotyledons of zucchini cv. Negro Belleza as described elsewhere (Pérez-García et al 2001). The cucurbit pathogenic bacterium Pseudomonas syringae pv.…”

Section: Microorganisms and Culture Conditionsmentioning

confidence: 99%

“…Rochet were used (Pérez-García et al 2001). Erlenmeyer flasks (1 l) containing 200 ml of nutrient broth (Bacillus) or KB (Pseudomonas) were inoculated with 500 μl fresh cultures of the different bacteria, and incubated overnight in an orbital shaker at 28°C and 200 rpm.…”

Section: Plant Growth Promotion Assaysmentioning

confidence: 99%

“…Root bacterization was performed by dipping the roots of melon seedlings into the corresponding cell suspension for 30 min (Cazorla et al 2006). The bacterized plants were then transferred to plastic pots with the appropriate substrate and incubated in a growth chamber under conditions described elsewhere (Pérez-García et al 2001). Fifteen days after incubation, the fresh weight of the aerial parts of the plants was recorded, and the percent increase of fresh weight was calculated by comparison with untreated controls.…”

Section: Plant Growth Promotion Assaysmentioning

confidence: 99%

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Isolation and selection of plant growth-promoting rhizobacteria as inducers of systemic resistance in melon

et al. 2012

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Backgroud and aims Powdery mildew elicited by Podosphaera fusca is an important threat to cucurbits. In order to find alternatives to the current use of chemicals, we examined the potential use of plant growth-promoting rhizobacteria (PGPR) for controlling the disease by induction of systemic resistance in the host plant. Methods A collection of Bacillus and Pseudomonas strains from different origins was studied, including strains isolated from roots of disease-free melon plants obtained from a greenhouse plagued by powdery mildew. The selection of best candidates was based on the evaluation of different traits commonly associated with PGPR, such as antifungal and siderophore production, swimming and swarming motilities, biofilm formation, auxin production and promotion of root development.Results Three Bacillus strains, B. subtilis UMAF6614 and UMAF6639 and B. cereus UMAF8564, and two Pseudomonas fluorescens strains, UMAF6031 and UMAF6033, were selected after ranking the strains using a nonparametric statistics test. Applied to melon seedlings, the selected strains were able to promote plant growth, increasing fresh weight up to 30%. Furthermore, these strains provided protection against powdery mildew and also against angular leaf spot caused by Pseudomonas syringae pv. lachrymans, with disease reductions of up to 60%. Conclusions These results suggest that the use of ISRpromoting PGPR could be a promising strategy for the integrated control of cucurbit powdery mildew and other cucurbit diseases.

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“…Since a few mildew colonizations appeared on resistant lines when F 3 plants seedlings were used to test the resistance in artificial climate box, while no mildew colonizations appeared on leaves of resistant plant in the field experiment, so the resistance test of F 2 plant was chosen to do in the field. The inoculation condition affecting the outcome of resistance to powdery mildew race 1 was reported previously (Pérez-García et al 2001).…”

Section: Discussionmentioning

confidence: 87%

Powdery mildew resistance gene (Pm-AN) located in a segregation distortion region of melon LGV

et al. 2011

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Development of Sphaerotheca fusca on susceptible, resistant, and temperature-sensitive resistant melon cultivars

Cited by 31 publications

References 20 publications

Development ofErysiphe pulchra, the causal agent of powdery mildew, on leaf disks of susceptible and resistant flowering dogwood (Cornus florida)

Development ofErysiphe pulchra, the causal agent of powdery mildew, on leaf disks of susceptible and resistant flowering dogwood (Cornus florida)

Isolation and selection of plant growth-promoting rhizobacteria as inducers of systemic resistance in melon

Powdery mildew resistance gene (Pm-AN) located in a segregation distortion region of melon LGV

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