Development of Spirulina sea-weed raw extract/polyamidoamine hydrogel system as novel platform in photodynamic therapy: Photostability and photoactivity of chlorophyll a

Rizzi, Vito; Gubitosa, Jennifer; Fini, Paola; Fraix, Aurore; Sortino, Salvatore; Agostiano, Angela; Cosma, Pinalysa

doi:10.1016/j.msec.2020.111593

Cited by 16 publications

(16 citation statements)

References 48 publications

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“…2,46 Design and preparation of chitosan and chitosan/snail slime blended films. Chitosan films were obtained by modifying the procedure of Rizzi et al 4,14,18,19 and Gubitosa et al, 47 as detailed in the following. Low molecular weight chitosan powder was dissolved in 0.8% (v/v) lactic acid aqueous solution to finally obtain a 1% (w/v) chitosan concentration after maintaining it under continuous stirring for 24 h. Glycerol was added as a plasticizing agent at 0.5%.…”

Section: Methodsmentioning

confidence: 99%

Chitosan/snail slime films as multifunctional platforms for potential biomedical and cosmetic applications: physical and chemical characterization

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Chitosan/snail slime films as multifunctional platforms for potential biomedical and cosmetic applications: physical and chemical characterization

et al. 2023

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“…Dilakukan disentrifugasi selama 20 menit dengan kecepatan 3000 rpm dan dipisahkan antara endapan dengan supernatannya (Ranjitha et al, 2020). Supernatan dilakukan pengukuran OD dengan spektrofotometri pada panjang gelombang 450 nm, 663 nm, dan 645 nm (Rizzi et al, 2021). Kandungan klorofil a, b, total dan karotenoid dihitung menggunakan persamaan:…”

Section: Pemanenan Dan Perhitungan Produksi Biomassaunclassified

“…Panjang gelombang yang tepat dan optimal, akan meningkatkan proses metabolisme sel dan pembentukan klorofi. Semakin tinggiya kadar klorofil akan menyebabkan proses fotosintesis berjalan dengan maksimal sehingga laju pertumbuhan sel spirulina meningkat eksponensial (Rizzi et al, 2021). Terdapat korelasi linier positif antara kepadatan sel mikroalga terhadap kadar klorofil yaitu semakin tinggi kepadatan sel atau biomassa maka kadar klorofil semakin tinggi.…”

Section: Kadar Klorofil Spirulina Platensisunclassified

Pertumbuhan, Kadar Pigmen dan Aktivitas Antioksidan Spirulina platensis pada Kultur dengan Perbedaan Warna Pencahayaan Leds

2022

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Antioksidan diperlukan tubuh untuk melindungi kesehatan kulit kita. Salah satu sumber antioksidan adalah mikroalga Spirulina sp. Permasalahan pemanfaatan spirulina adalah diperlukan teknologi kultur yang tepat untuk menghasilkan aktivitas antioksidan tinggi. Tujuan penelitian adalah melakukan optimasi pencahayaan (warna cahaya) dalam kultur spirulina untuk mendapatkan pertumbuhan terbaik, kadar pigmen dan aktivitas antioksidan tertinggi. Penelitian menggunakan metode eksperimen laboratoris dengan 5 perlakuan yaitu pemberian pencahaaan dengan LED warna biru, hijau, merah, putih dan lampu TL (fluoresens). Perlakuan perbedaan warna pencahayaan dalam kultur berpengaruh nyata terhadap pertumbuhan, kadar pigmen dan aktivitas antioksidan. Perlakuan terbaik adalah pemberian pencahayaan LED merah yaitu Spirulina platensis memiliki pertumbuhan mutlak sebesar 1,602 d x 104 sel/ml, laju pertumbuhan 0,108 hari-1, jumlah generasi 2,028, waktu generasi 6,416 hari, yield 9,649 gr/gr, dan produktifitas 24,740 gr/L.hari. Ekstrak spirulina mengandung klorofil a sebesar 10,153 mg/L, klorofil b sebesar 4,744 mg/L, total klorofil 16,053 mg/L, karotenoid 5,893 mg/L. selain itu juga memiliki kandungan pigmen fikobiliprotein yaitu fikosianin 0,055 mg/L, alofikosianin 0,037 mg/L dan fikoeritrin 0,218 mg/L. eksrak spirulina dari hasil kultur dengan pencahayaan LED merah memiliki aktivitas antioksidan dengan nilai IC50 sebesar 105,4 ppm. Aktivitas antioksidan dari ekstrak sepirulina di karenakan adanya kandungan senyawa fitokimia dan pigmen. Antioxidants are needed by the body to protect the health of our skin. One source of antioxidants is the microalgae spirulina sp. The problem of using spirulina is that appropriate culture technology is needed to produce high antioxidant activity. The aim of the research was to optimize lighting (light color) in spirulina culture to get the best growth, highest pigment content and antioxidant activity. The study used a laboratory experimental method with 5 treatments, lighting with blue, green, red, white LEDs and fluorescent lights. The treatment of different lighting colors in culture had a significant effect on growth, pigment levels and antioxidant activity. The best treatment was the provision of red LED lighting, Spirulina platensis which had absolute growth of 1.602 d x 104 cells/ml, growth rate of 0.108 day-1 , number of generations 2,028, generation time of 6.416 days, yield of 9.649 gr/gr, and productivity of 24.740 gr/L.day. Spirulina extract contains chlorophyll a of 10,153 mg/L, chlorophyll b of 4,744 mg/L, total chlorophyll 16,053 mg/L, carotenoids 5,893 mg/L. In addition, it also contains phycobiliprotein pigments, phycocyanin 0.055 mg/L, allophycocyanin 0.037 mg/L and phycoerythrin 0.218 mg/L. Spirulina extract from culture results with red LED lighting has antioxidant activity with an IC50 value of 105.4 ppm. The antioxidant activity of the sepirulina extract is due to the presence of phytochemical compounds and pigments.

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“…setelah inkubasi, sampel masingmasing dilakukan sentrifugasi dengan kecepatan 3000 rpm selama 20 menit (Ranjitha et al, 2020). Supernatan dilakukan spektrofotometri pada panjang gelombang 450 nm, 663 nm, dan 645 nm (Rizzi et al, 2021). Data Optical Density yang didapatkan digunakan untuk menghitung kadar klorofil a, b dan karotenoid.…”

Section: Penentuan Kadar Klorofil Dan Karotenoidunclassified

Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan

2022

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Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein. Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. The DPPH radical scavenging activity of the extract was due to the presence of antioxidant compounds from chlorophyll, carotenoids and phycobiliproteins.

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Development of Spirulina sea-weed raw extract/polyamidoamine hydrogel system as novel platform in photodynamic therapy: Photostability and photoactivity of chlorophyll a

Cited by 16 publications

References 48 publications

Chitosan/snail slime films as multifunctional platforms for potential biomedical and cosmetic applications: physical and chemical characterization

Chitosan/snail slime films as multifunctional platforms for potential biomedical and cosmetic applications: physical and chemical characterization

Pertumbuhan, Kadar Pigmen dan Aktivitas Antioksidan Spirulina platensis pada Kultur dengan Perbedaan Warna Pencahayaan Leds

Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan

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