Development of supermacroporous monolithic adsorbents for purifying lectins by affinity with sugars

Gonçalves, Gabriel Ramos Ferreira; Gandolfi, Olga Reinert Ramos; Santos, Carilan Moreira Souza; Bonomo, Renata Cristina Ferreira; Veloso, Cristiane Martins; Fontan, Rafael da Costa Ilhéu

doi:10.1016/j.jchromb.2016.09.016

Cited by 33 publications

(46 citation statements)

References 31 publications

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“…Another property analyzed was the expansion degree (ED) of the cryogel, which is useful for converting between the dehydrated mass of the cryogel (when in storage) and its hydrated volume (when in use) . Ten of each cryogel (pAam and pAam‐SO 3 cryogels) were dehydrated and weighed on an analytical balance.…”

Section: Methodsmentioning

confidence: 99%

“…Characterization of these cryogels is of great importance in order to understand fluid flow dynamics in their interior, as well as the process of interactions between active sites with molecules in solution to predict the behavior of the process under analysis. Physical (porosity and pore size), hydrodynamic (permeability, residence time distribution, and axial dispersion), and chemical (grafting degree, number of active sites) aspects have been reported by various authors, correlated with alterations in the processes of obtaining the cryogels, in relation to either the cryogenic routes used or their compositions .…”

Section: Introductionmentioning

confidence: 94%

“…The development and use of polymeric monoliths is growing, with a significant increase in the number of papers on these materials over the last two decades . Polyacrylamide monoliths (pAam cryogel) stand out among the polymers used and are obtained by the polymerization of acrylamide (Aam) with N , N ′‐methylene‐ bis ‐acrylamide (BAam), with the addition of other monomers, such as allyl glycidyl ether (AGE), under moderate freezing conditions using the technique known as “cryogelling.” These monoliths are known as cryogels and their use has been reported by various authors .…”

Section: Introductionmentioning

confidence: 99%

“…Hence, research on modifying cryogel structures represent a promising area of study and is developing rapidly. Chemical or physical modifications can be done to increase the efficiency of the separation processes .…”

Section: Introductionmentioning

confidence: 99%

“…Considering the characteristics of the pAAm cryogels, their use in the form of a fixed bed in the purification of biocompounds from concentrated or nonclarified media presents great potential . Many of the cryogel characteristics, however, are estimated in batches using techniques that involve considerable errors, since they depend on the subjective characteristics of the analyst.…”

Section: Introductionmentioning

confidence: 99%

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Alternatives for characterizing macroporous polyacrylamide monolithic ion exchanger columns

Fontan

Bonomo

Gonçalves

et al. 2017

Polymer Engineering & Sci

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The development of new adsorbents to separate biocompounds and polyacrylamide monolithic cryogels shows great potential for purifying macromolecules from non‐clarified media. In this work, alternatives for functionalization and characterization of polyacrylamide monoliths grafted with 2‐acrylamide‐2‐methyl‐1‐propanosulfonic acid were explored. Dynamic grafting did not affect swelling capacity, or graft density. Pore size was evaluated by optical microscopy, which presented the same values obtained by scanning electronic microscopy. The method of moments was used to determine porosity, apparent axial dispersion coefficient, and the height of the theoretical plates of the columns, presenting results consistent with those in the literature. The permeability constant was determined by varying the viscosity of a Newtonian fluid at constant pressure, with the value being the same as that obtained by varying the hydrostatic pressure over the column. Results demonstrated these techniques were adequate for characterizing polyacrylamide cryogels, opening new perspectives in this field. POLYM. ENG. SCI., 58:1717–1725, 2018. © 2017 Society of Plastics Engineers

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Alternatives for characterizing macroporous polyacrylamide monolithic ion exchanger columns

Fontan

Bonomo

Gonçalves

et al. 2017

Polymer Engineering & Sci

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Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Silva

Nascimento

et al. 2019

J of Applied Polymer Sci

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The use of macroporous monolithic matrices in the purification of biocompounds is constantly growing and developing. In this work, the objective was to optimize the quantity of N-acetyl-D-glucosamine (D-GlcNAc) immobilized on the surface of macroporous polymeric cryogels for capture of lectins from less clarified solutions. Surface response methodology was applied and it was observed that the immobilization temperature of the glutaraldehyde (GLU) and the D-GlcNAc concentration influenced the amount of sugar immobilized. The matrices produced with 1.1% of allyl glycidyl ether were functionalized by GLU. Optimal maximum condition was obtained with mean value of 160.39 AE 26.38 mg of D-GlcNAc immobilized per gram of dry cryogel. Characterization analyses of the matrices showed that the activation process was effective, maintaining the macroporous structure and physical characteristics. The adsorbents produced were tested for capture of lectins from a crude protein solution of barley. At tested conditions, adsorbent capture around 11% of protein in solution but reduce the hemaglutinating capacity in 40%, demonstrating its selectivity. The cryogels functionalized with D-GlcNAc present potential for use in capture compounds by affinity with carbohydrates, such as lectins.

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Performance of pseudo‐specific cryogel in lysozyme purification from chicken egg white

et al. 2022

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The application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with L-phenylalanine (cryogel-Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier-transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mg phenylalanine g cryogel À1 . Cryogel-Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200 C. Differential scanning calorimetry and X-ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 Â 10 À13 m 2 , axial dispersion ranging from 10 À7 to 10 À6 m 2 s À1 , and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g À1 ) was obtained using sodium thiocyanate saline solution (0.025 mol L À1 , pH 5.0). The ability of the cryogel-Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml À1 ), purity (87.92%), purification factor (11.49), and sulphate-polyacrylamide electrophoresis gel (SDS-PAGE) electrophoresis gel.

show abstract

Development of supermacroporous monolithic adsorbents for purifying lectins by affinity with sugars

Cited by 33 publications

References 31 publications

Alternatives for characterizing macroporous polyacrylamide monolithic ion exchanger columns

Alternatives for characterizing macroporous polyacrylamide monolithic ion exchanger columns

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Performance of pseudo‐specific cryogel in lysozyme purification from chicken egg white

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