Development of tailor-made glycidyl methacrylate-divinyl benzene copolymer for immobilization of d-amino acid oxidase from Aspergillus species strain 020 and its application in the bioconversion of cephalosporin C

Mujawar, Salim K.; Kotha, Arika; Rajan, C. R.; Ponrathnam, S.; Shewale, Jaiprakash G.

doi:10.1016/s0168-1656(99)00124-8

Cited by 15 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glycidyl methacrylate (GMA) was copolymerized with divinylbenzene (DVB) ( Figure 1c) by free radical polymerization in the presence of ␣,␣=-azoisobutyronitrile (AIBN) as thermal initiator [19,20]. The precipitation polymerization was performed in ACN and yielded strongly crosslinked beads featuring epoxide groups on the surface.…”

Section: Particle Preparation and Derivatizationmentioning

confidence: 99%

Material-enhanced laser desorption/ionization (MELDI)—A new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis

Feuerstein

Najam‐ul‐Haq

Rainer

et al. 2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Over the past couple of years, proteomics pattern analysis has emerged as an effective method for the early diagnosis of diseases such as ovarian, breast, or prostate cancer, without identification of single biomarkers. MALDI-TOF MS, for example, offers a simple approach for fast and reliable protein profiling, especially by using carrier materials with various physical and chemical properties, in combination with a MALDI matrix. This approach is referred to as materialenhanced laser desorption/ionization (MELDI). In this paper, we report the development and application of derivatized carrier materials [cellulose, silica, poly(glycidyl methacrylate/divinylbenzene) (GMA/DVB) particles, and diamond powder] for fast and direct MALDI-TOF MS protein profiling. The applicability of MELDI for rapid protein profiling was evaluated with human serum samples. These carriers, having various hydrophobicities, resulted in characteristic mass fingerprints, even if all materials were derivatized with iminodiacetic acid (IDA) to yield an immobilized metal affinity chromatography (IMAC) functionality. Our study demonstrates that analyzing complex biological samples, such as human serum, by employing different MELDI carrier materials yielded type-and size-dependent performance variation. T he quest to diagnose and identify diseases at early stages depends mainly on the accurate determination of the marker entities in the biological fluids. These marker entities are normally very low concentrated [1], and highly sensitive methods are required to bind them effectively from the biosamples and to measure them with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) [2]. MALDI mass spectrometry, in recent times, has proven to be an authentic technique to analyze peptides and proteins [3,4]. These biomolecules out of the complex biofluids like serum are difficult to ionize and thus, bring into time of flight (TOF) because of the complexity of the sample and the suppressing nonvolatile salts [5]. The aforementioned problems are tackled by another technique introduced years ago, namely, surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS), for the detection of several peptides and proteins from complex biological specimens [6 -8]. This affinity-based method utilizes selective adsorption of proteins on various chromatographic surfaces to reduce sample complexity [9,10]. Spectra generated by SELDI-TOF MS usually show limited peak and binding capacity due to the smaller surface area of the plane protein chip [11]. Limitations of SELDI are also associated with the lower mass resolution of the applied MS instrument that

show abstract

Section: Particle Preparation and Derivatizationmentioning

confidence: 99%

Material-enhanced laser desorption/ionization (MELDI)—A new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis

Feuerstein

Najam‐ul‐Haq

Rainer

et al. 2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…It is indicated that the presence of hydrogen peroxide can reduce the catalytic efficiency of DAAOs [21]. To remove hydrogen peroxide, DAAO catalysis has been used together with catalase and horseradish peroxidase [22][23][24], and catalase was co-immobilized with DAAOs on a support [8,9,25,26].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Genetically-Modified d-Amino Acid Oxidase and Catalase on Carbon Nanotubes to Improve the Catalytic Efficiency

Sun

et al. 2016

Catalysts

View full text Add to dashboard Cite

D-amino acid oxidase (DAAO) and catalase (CAT) have been genetically modified by fusing them to an elastin-like polypeptide (ELP). ELP-DAAO and ELP-CAT have been separately immobilized on multi-walled carbon nanotubes (MWNTs). It has been found that the secondary structures of the enzymes have been preserved. ELP-DAAO catalyzed the oxidative deamination of D-alanine, and H 2 O 2 was evolved continuously. When the MWNT-supported enzymes were used together, the generated hydrogen peroxide of ELP-DAAO could be decomposed in situ. The catalytic efficiency of the two immobilized enzymes was more than five times greater than that of free ELP-DAAO when the ratio of immobilized ELP-CAT to immobilized ELP-DAAO was larger than 1:1.

show abstract

“…The addition of nucleophiles to an epoxy ring plays also a significant role in the chemical modification of polymer materials. Many useful materials were obtained previously as a consequence of chemical transformations of epoxy group bearing polymers under the influence of amines, for instance, polymer supported enzymes,4, 5 amino acids,6 proteins,7 and catalysts,8–11 sorbents for metal ions12–18 or surface‐reactive acrylic copolymers for fabrication of microfluidic devices for chemical analysis 19…”

Section: Introductionmentioning

confidence: 99%

Polyamine functionalized glycidyl methacrylate terpolymers as scavengers for metal ions uptake from organic solutions

Bukowska

Bukowski

2011

J of Applied Polymer Sci

View full text Add to dashboard Cite

Resins with amine functionalities were obtained as a result of chemical modification of low crosslinked terpolymers of glycidyl methacrylate with styrene and divinylbenzene or diethylene glycol dimethacrylate. The loading of amine groups, swelling in the selected organic solvents, and thermal properties of the resins were studied. FTIR, DSC, TGA, SEM, and stereomicroscopy methods were also applied for exploring of the resins.The amine functionalized resins were tested as sorbents for uptake of cobalt(II), copper(II), iron(III), and manganese(II) ions from methanol and its mixtures with methylene chloride. The study show that all polyamine functionalized resins uptake quickly Cu(II) ions from methanol solutions. Their ability to remove cobalt(II), iron(III), and manganese(II) ions from solution depended on the mutual compatibility of solvents and resins. It increases clearly when the mixtures of methanol and methylene chloride are applied. V C 2011 Wiley Periodicals, Inc. J Appl Polym Sci 124: [904][905][906][907][908][909][910][911][912][913][914] 2012

show abstract

Development of tailor-made glycidyl methacrylate-divinyl benzene copolymer for immobilization of d-amino acid oxidase from Aspergillus species strain 020 and its application in the bioconversion of cephalosporin C

Cited by 15 publications

References 21 publications

Material-enhanced laser desorption/ionization (MELDI)—A new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis

Material-enhanced laser desorption/ionization (MELDI)—A new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis

Immobilization of Genetically-Modified d-Amino Acid Oxidase and Catalase on Carbon Nanotubes to Improve the Catalytic Efficiency

Polyamine functionalized glycidyl methacrylate terpolymers as scavengers for metal ions uptake from organic solutions

Contact Info

Product

Resources

About